Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoida...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-11-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/11/6818 |
| _version_ | 1811262927322218496 |
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| author | Panchanathan Manivasagan Junghwan Oh |
| author_facet | Panchanathan Manivasagan Junghwan Oh |
| author_sort | Panchanathan Manivasagan |
| collection | DOAJ |
| description | Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine. |
| first_indexed | 2024-04-12T19:35:29Z |
| format | Article |
| id | doaj.art-ae10025bbce14c9aa300e5c6082e9501 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:35:29Z |
| publishDate | 2015-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-ae10025bbce14c9aa300e5c6082e95012022-12-22T03:19:14ZengMDPI AGMarine Drugs1660-33972015-11-0113116818683710.3390/md13116818md13116818Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa CellsPanchanathan Manivasagan0Junghwan Oh1Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, KoreaMarine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, KoreaMarine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.http://www.mdpi.com/1660-3397/13/11/6818fucoidanfucoidanaseStreptomycesgold nanoparticlesHeLa cells |
| spellingShingle | Panchanathan Manivasagan Junghwan Oh Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells Marine Drugs fucoidan fucoidanase Streptomyces gold nanoparticles HeLa cells |
| title | Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells |
| title_full | Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells |
| title_fullStr | Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells |
| title_full_unstemmed | Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells |
| title_short | Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells |
| title_sort | production of a novel fucoidanase for the green synthesis of gold nanoparticles by streptomyces sp and its cytotoxic effect on hela cells |
| topic | fucoidan fucoidanase Streptomyces gold nanoparticles HeLa cells |
| url | http://www.mdpi.com/1660-3397/13/11/6818 |
| work_keys_str_mv | AT panchanathanmanivasagan productionofanovelfucoidanaseforthegreensynthesisofgoldnanoparticlesbystreptomycesspanditscytotoxiceffectonhelacells AT junghwanoh productionofanovelfucoidanaseforthegreensynthesisofgoldnanoparticlesbystreptomycesspanditscytotoxiceffectonhelacells |