Baicalin regulates the dopamine system to control the core symptoms of ADHD
Abstract We aimed to test the therapeutic effects of baicalin on attention deficit hyperactivity disorder (ADHD) in an animal model and to explain the potential mechanism. We investigated the therapeutic effects and mechanisms of baicalin in a spontaneously hypertensive rat (SHR) model of ADHD depen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13041-019-0428-5 |
| _version_ | 1818998969809764352 |
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| author | Rongyi Zhou Jiaojiao Wang Xinmin Han Bingxiang Ma Haixia Yuan Yuchen Song |
| author_facet | Rongyi Zhou Jiaojiao Wang Xinmin Han Bingxiang Ma Haixia Yuan Yuchen Song |
| author_sort | Rongyi Zhou |
| collection | DOAJ |
| description | Abstract We aimed to test the therapeutic effects of baicalin on attention deficit hyperactivity disorder (ADHD) in an animal model and to explain the potential mechanism. We investigated the therapeutic effects and mechanisms of baicalin in a spontaneously hypertensive rat (SHR) model of ADHD depending on the dopamine (DA) deficit theory. In this study, fifty SHRs were randomly divided into five groups: methylphenidate (MPH), baicalin (50 mg/kg, 100 mg/kg, or 150 mg/kg), and saline-treated. Ten Wistar Kyoto (WKY) rats were used as controls. All rats were orally administered the treatment for four weeks. Motor activity, spatial learning and memory ability were assessed with the open-field and Morris water-maze tests. The mRNA and protein levels of tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), synaptosomal-associated protein of molecular mass 25kD (SNAP25) and synataxin 1a in synaptosomes were detected with real-time polymerase chain reaction (PCR) and Western blot. In addition, DA levels were measured in the prefrontal cortex and striatum. The results indicated that both MPH and baicalin at doses of 150 mg/kg and 100 mg/kg significantly decreased the hyperactivity and improved the spatial learning memory deficit in the SHRs and increased the synaptosomal mRNA and protein levels of TH, SNAP25, VMAT2 and synataxin 1a compared with saline treatment. MPH significantly increased DA levels in both the prefrontal cortex (PFC) and striatum, while baicalin significantly increased DA levels only in the striatum. The results of the present study showed that baicalin treatment was effective for controlling the core symptoms of ADHD. Baicalin increased DA levels only in the striatum, which suggested that baicalin may target the striatum. The increased DA levels may partially be attributed to the increased mRNA and protein expression of TH, SNAP25, VMAT2, and syntaxin 1a. Therefore, these results suggested that the pharmacological effects of baicalin were associated with the synthesis, vesicular localization, and release of DA and might be effective in treating ADHD. However, further studies are required to better understand the molecular mechanisms underlying these findings. |
| first_indexed | 2024-12-20T22:09:59Z |
| format | Article |
| id | doaj.art-ae1097157ed44457b20a0add899d8183 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-20T22:09:59Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-ae1097157ed44457b20a0add899d81832022-12-21T19:25:11ZengBMCMolecular Brain1756-66062019-02-0112111410.1186/s13041-019-0428-5Baicalin regulates the dopamine system to control the core symptoms of ADHDRongyi Zhou0Jiaojiao Wang1Xinmin Han2Bingxiang Ma3Haixia Yuan4Yuchen Song5The First Affiliated Hospital of Henan University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineThe First Affiliated Hospital of Henan University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineAbstract We aimed to test the therapeutic effects of baicalin on attention deficit hyperactivity disorder (ADHD) in an animal model and to explain the potential mechanism. We investigated the therapeutic effects and mechanisms of baicalin in a spontaneously hypertensive rat (SHR) model of ADHD depending on the dopamine (DA) deficit theory. In this study, fifty SHRs were randomly divided into five groups: methylphenidate (MPH), baicalin (50 mg/kg, 100 mg/kg, or 150 mg/kg), and saline-treated. Ten Wistar Kyoto (WKY) rats were used as controls. All rats were orally administered the treatment for four weeks. Motor activity, spatial learning and memory ability were assessed with the open-field and Morris water-maze tests. The mRNA and protein levels of tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), synaptosomal-associated protein of molecular mass 25kD (SNAP25) and synataxin 1a in synaptosomes were detected with real-time polymerase chain reaction (PCR) and Western blot. In addition, DA levels were measured in the prefrontal cortex and striatum. The results indicated that both MPH and baicalin at doses of 150 mg/kg and 100 mg/kg significantly decreased the hyperactivity and improved the spatial learning memory deficit in the SHRs and increased the synaptosomal mRNA and protein levels of TH, SNAP25, VMAT2 and synataxin 1a compared with saline treatment. MPH significantly increased DA levels in both the prefrontal cortex (PFC) and striatum, while baicalin significantly increased DA levels only in the striatum. The results of the present study showed that baicalin treatment was effective for controlling the core symptoms of ADHD. Baicalin increased DA levels only in the striatum, which suggested that baicalin may target the striatum. The increased DA levels may partially be attributed to the increased mRNA and protein expression of TH, SNAP25, VMAT2, and syntaxin 1a. Therefore, these results suggested that the pharmacological effects of baicalin were associated with the synthesis, vesicular localization, and release of DA and might be effective in treating ADHD. However, further studies are required to better understand the molecular mechanisms underlying these findings.http://link.springer.com/article/10.1186/s13041-019-0428-5BaicalinADHDDopamineSpontaneously hypertensive ratsBehavioral tests |
| spellingShingle | Rongyi Zhou Jiaojiao Wang Xinmin Han Bingxiang Ma Haixia Yuan Yuchen Song Baicalin regulates the dopamine system to control the core symptoms of ADHD Molecular Brain Baicalin ADHD Dopamine Spontaneously hypertensive rats Behavioral tests |
| title | Baicalin regulates the dopamine system to control the core symptoms of ADHD |
| title_full | Baicalin regulates the dopamine system to control the core symptoms of ADHD |
| title_fullStr | Baicalin regulates the dopamine system to control the core symptoms of ADHD |
| title_full_unstemmed | Baicalin regulates the dopamine system to control the core symptoms of ADHD |
| title_short | Baicalin regulates the dopamine system to control the core symptoms of ADHD |
| title_sort | baicalin regulates the dopamine system to control the core symptoms of adhd |
| topic | Baicalin ADHD Dopamine Spontaneously hypertensive rats Behavioral tests |
| url | http://link.springer.com/article/10.1186/s13041-019-0428-5 |
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