Changes in thickness of each layer of developing chicken cornea after administration of caffeine.
The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Via Medica
2010-08-01
|
| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4221 |
| _version_ | 1830138621880958976 |
|---|---|
| author | Hieronim Bartel Dariusz Tosik Monika Kujawa-Hadryś |
| author_facet | Hieronim Bartel Dariusz Tosik Monika Kujawa-Hadryś |
| author_sort | Hieronim Bartel |
| collection | DOAJ |
| description | The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated - 3.5 mg/egg. In 36th hour of incubation solutions were given with cannula through a hole in an egg shell directly onto amniotic membrane. After closing the hole with paraffin, eggs were put back into incubator. On 10th and 19th day of incubation corneas were taken for morphometric and morphological analysis. In experimental groups reduction of corneal thickness, thickening of corneal epithelium and corneal endothelium as well as Bowman's and Descemet's membranes, decrease of thickness of corneal stroma in comparison with the control group have been observed. Caffeine causes thickness changes of all layers and decreases the total thickness of a developing cornea. |
| first_indexed | 2024-12-17T08:28:39Z |
| format | Article |
| id | doaj.art-ae17a1067c134e888fa6daa1e4df31ee |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-17T08:28:39Z |
| publishDate | 2010-08-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-ae17a1067c134e888fa6daa1e4df31ee2022-12-21T21:56:40ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312010-08-0148227327710.5603/4221Changes in thickness of each layer of developing chicken cornea after administration of caffeine.Hieronim BartelDariusz TosikMonika Kujawa-HadryśThe aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated - 3.5 mg/egg. In 36th hour of incubation solutions were given with cannula through a hole in an egg shell directly onto amniotic membrane. After closing the hole with paraffin, eggs were put back into incubator. On 10th and 19th day of incubation corneas were taken for morphometric and morphological analysis. In experimental groups reduction of corneal thickness, thickening of corneal epithelium and corneal endothelium as well as Bowman's and Descemet's membranes, decrease of thickness of corneal stroma in comparison with the control group have been observed. Caffeine causes thickness changes of all layers and decreases the total thickness of a developing cornea.http://czasopisma.viamedica.pl/fhc/article/view/4221 |
| spellingShingle | Hieronim Bartel Dariusz Tosik Monika Kujawa-Hadryś Changes in thickness of each layer of developing chicken cornea after administration of caffeine. Folia Histochemica et Cytobiologica |
| title | Changes in thickness of each layer of developing chicken cornea after administration of caffeine. |
| title_full | Changes in thickness of each layer of developing chicken cornea after administration of caffeine. |
| title_fullStr | Changes in thickness of each layer of developing chicken cornea after administration of caffeine. |
| title_full_unstemmed | Changes in thickness of each layer of developing chicken cornea after administration of caffeine. |
| title_short | Changes in thickness of each layer of developing chicken cornea after administration of caffeine. |
| title_sort | changes in thickness of each layer of developing chicken cornea after administration of caffeine |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4221 |
| work_keys_str_mv | AT hieronimbartel changesinthicknessofeachlayerofdevelopingchickencorneaafteradministrationofcaffeine AT dariusztosik changesinthicknessofeachlayerofdevelopingchickencorneaafteradministrationofcaffeine AT monikakujawahadrys changesinthicknessofeachlayerofdevelopingchickencorneaafteradministrationofcaffeine |