Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury
This study was designed to compare the roles of botulinum neurotoxin A (BoNT/A) and extracorporeal shock wave therapy (ESWT) in promoting the functional recovery and regeneration of injured peripheral nerves. A total of 45 six-week-old rats with sciatic nerve injury were randomly divided into two ex...
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MDPI AG
2021-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/12/879 |
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| author | Minsu Seo Dongin Lim Shengshu Kim Taeyeon Kim Bum Sun Kwon Kiyeun Nam |
| author_facet | Minsu Seo Dongin Lim Shengshu Kim Taeyeon Kim Bum Sun Kwon Kiyeun Nam |
| author_sort | Minsu Seo |
| collection | DOAJ |
| description | This study was designed to compare the roles of botulinum neurotoxin A (BoNT/A) and extracorporeal shock wave therapy (ESWT) in promoting the functional recovery and regeneration of injured peripheral nerves. A total of 45 six-week-old rats with sciatic nerve injury were randomly divided into two experimental groups and one control group. The experimental groups received a single session of intranerve BoNT/A or ESWT immediately after a nerve-crushing injury. The control group was not exposed to any treatment. Differentiation of Schwann cells and axonal sprouting were observed through immunofluorescence staining, ELISA, real-time PCR, and Western blot at 3, 6, and 10 weeks post-nerve injury. For clinical assessment, serial sciatic functional index analysis and electrophysiological studies were performed. A higher expression of GFAP and S100β was detected in injured nerves treated with BoNT/A or ESWT. The levels of GAP43, ATF3, and NF200 associated with axonal regeneration in the experimental groups were also significantly higher than in the control group. The motor functional improvement occurred after 7 weeks of clinical observation following BoNT/A and ESWT. Compared with the control group, the amplitude of the compound muscle action potential in the experimental groups was significantly higher from 6 to 10 weeks. Collectively, these findings indicate that BoNT/A and ESWT similarly induced the activation of Schwann cells with the axonal regeneration of and functional improvement in the injured nerve. |
| first_indexed | 2024-03-10T03:58:18Z |
| format | Article |
| id | doaj.art-ae17bd22a60b442d9c2c7a3d9bddc09f |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T03:58:18Z |
| publishDate | 2021-12-01 |
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| series | Toxins |
| spelling | doaj.art-ae17bd22a60b442d9c2c7a3d9bddc09f2023-11-23T10:50:51ZengMDPI AGToxins2072-66512021-12-01131287910.3390/toxins13120879Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve InjuryMinsu Seo0Dongin Lim1Shengshu Kim2Taeyeon Kim3Bum Sun Kwon4Kiyeun Nam5Department of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, KoreaThis study was designed to compare the roles of botulinum neurotoxin A (BoNT/A) and extracorporeal shock wave therapy (ESWT) in promoting the functional recovery and regeneration of injured peripheral nerves. A total of 45 six-week-old rats with sciatic nerve injury were randomly divided into two experimental groups and one control group. The experimental groups received a single session of intranerve BoNT/A or ESWT immediately after a nerve-crushing injury. The control group was not exposed to any treatment. Differentiation of Schwann cells and axonal sprouting were observed through immunofluorescence staining, ELISA, real-time PCR, and Western blot at 3, 6, and 10 weeks post-nerve injury. For clinical assessment, serial sciatic functional index analysis and electrophysiological studies were performed. A higher expression of GFAP and S100β was detected in injured nerves treated with BoNT/A or ESWT. The levels of GAP43, ATF3, and NF200 associated with axonal regeneration in the experimental groups were also significantly higher than in the control group. The motor functional improvement occurred after 7 weeks of clinical observation following BoNT/A and ESWT. Compared with the control group, the amplitude of the compound muscle action potential in the experimental groups was significantly higher from 6 to 10 weeks. Collectively, these findings indicate that BoNT/A and ESWT similarly induced the activation of Schwann cells with the axonal regeneration of and functional improvement in the injured nerve.https://www.mdpi.com/2072-6651/13/12/879botulinum toxinshock waveperipheral nerve injuryneural regenerationfunctional recovery |
| spellingShingle | Minsu Seo Dongin Lim Shengshu Kim Taeyeon Kim Bum Sun Kwon Kiyeun Nam Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury Toxins botulinum toxin shock wave peripheral nerve injury neural regeneration functional recovery |
| title | Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_full | Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_fullStr | Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_full_unstemmed | Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_short | Effect of Botulinum Toxin Injection and Extracorporeal Shock Wave Therapy on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_sort | effect of botulinum toxin injection and extracorporeal shock wave therapy on nerve regeneration in rats with experimentally induced sciatic nerve injury |
| topic | botulinum toxin shock wave peripheral nerve injury neural regeneration functional recovery |
| url | https://www.mdpi.com/2072-6651/13/12/879 |
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