Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome
This study investigated the therapeutic potential of adlay on polycystic ovary syndrome (PCOS) and its possible underlying mechanism. The active anti-androgenic components in adlay were further analyzed. Rat ovarian theca cells were treated with human chorionic gonadotropin to stimulate androstenedi...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614003090 |
| _version_ | 1818412102829735936 |
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| author | Chi-Hao Wu Mei-Jou Chen Tzong-Ming Shieh Kai-Lee Wang Ya-Ting Wu Shih-Min Hsia Wenchang Chiang |
| author_facet | Chi-Hao Wu Mei-Jou Chen Tzong-Ming Shieh Kai-Lee Wang Ya-Ting Wu Shih-Min Hsia Wenchang Chiang |
| author_sort | Chi-Hao Wu |
| collection | DOAJ |
| description | This study investigated the therapeutic potential of adlay on polycystic ovary syndrome (PCOS) and its possible underlying mechanism. The active anti-androgenic components in adlay were further analyzed. Rat ovarian theca cells were treated with human chorionic gonadotropin to stimulate androstenedione (AD) secretion. The ethyl acetate subfraction obtained from the ethanolic extract of adlay hull (AHE-EA) exerted superior efficacy against AD production. Four major constituents in AHE-EA, including 5,7-dihydroxychromone, liquiritigenin, isoliquiritigenin, and homoeriodictyol, were identified by HPLC–MS and exhibited strong inhibition against high AD levels. In a DHEA-induced PCOS rat model, administration of AHE-EA significantly decreased serum AD levels, improved hyperglycemia and insulin resistance, and attenuated oxidative stress and inflammatory responses in the ovaries. Histopathological morphology of ovarian tissues confirmed that AHE-EA could restore the estrus cycles and normal ovarian histology. Mechanistic characterization demonstrated that AHE-EA inhibited DHEA-induced hyperandrogenemia through modulate steroidogenic acute regulatory protein-related steroidogenesis in theca cells. |
| first_indexed | 2024-12-14T10:41:59Z |
| format | Article |
| id | doaj.art-ae18ae1b337d417dace7c7d92c3ee975 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T10:41:59Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ae18ae1b337d417dace7c7d92c3ee9752022-12-21T23:05:40ZengElsevierJournal of Functional Foods1756-46462014-11-0111393406Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndromeChi-Hao Wu0Mei-Jou Chen1Tzong-Ming Shieh2Kai-Lee Wang3Ya-Ting Wu4Shih-Min Hsia5Wenchang Chiang6School of Nutrition and Health Sciences, Taipei Medical University, Taipei, TaiwanDepartment of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, TaiwanDepartment of Dental Hygiene, College of Health Care, China Medical University, Taichung, TaiwanCenter for Reproductive Medicine & Sciences, Taipei Medical University, Taipei, TaiwanInstitute of Food Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan; Corresponding author. School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan. Tel.: +886-2-27361661-6558; fax: +886-2-2737-3112.Institute of Food Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei, TaiwanThis study investigated the therapeutic potential of adlay on polycystic ovary syndrome (PCOS) and its possible underlying mechanism. The active anti-androgenic components in adlay were further analyzed. Rat ovarian theca cells were treated with human chorionic gonadotropin to stimulate androstenedione (AD) secretion. The ethyl acetate subfraction obtained from the ethanolic extract of adlay hull (AHE-EA) exerted superior efficacy against AD production. Four major constituents in AHE-EA, including 5,7-dihydroxychromone, liquiritigenin, isoliquiritigenin, and homoeriodictyol, were identified by HPLC–MS and exhibited strong inhibition against high AD levels. In a DHEA-induced PCOS rat model, administration of AHE-EA significantly decreased serum AD levels, improved hyperglycemia and insulin resistance, and attenuated oxidative stress and inflammatory responses in the ovaries. Histopathological morphology of ovarian tissues confirmed that AHE-EA could restore the estrus cycles and normal ovarian histology. Mechanistic characterization demonstrated that AHE-EA inhibited DHEA-induced hyperandrogenemia through modulate steroidogenic acute regulatory protein-related steroidogenesis in theca cells.http://www.sciencedirect.com/science/article/pii/S1756464614003090AdlayHyperandrogenemiaInsulin resistancePolycystic ovary syndromeDehydroepiandrosterone |
| spellingShingle | Chi-Hao Wu Mei-Jou Chen Tzong-Ming Shieh Kai-Lee Wang Ya-Ting Wu Shih-Min Hsia Wenchang Chiang Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome Journal of Functional Foods Adlay Hyperandrogenemia Insulin resistance Polycystic ovary syndrome Dehydroepiandrosterone |
| title | Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome |
| title_full | Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome |
| title_fullStr | Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome |
| title_full_unstemmed | Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome |
| title_short | Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome |
| title_sort | potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin treated theca cells and a rodent model of polycystic ovary syndrome |
| topic | Adlay Hyperandrogenemia Insulin resistance Polycystic ovary syndrome Dehydroepiandrosterone |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614003090 |
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