Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to <i>Blumeria graminis</i> f.sp. <i>tritici</i>
Powdery mildew disease caused by <i>Blumeria graminis</i> f.sp. <i>tritici</i> (<i>Bgt</i>) leads to severe economic losses in bread wheat (<i>Triticum aestivum</i> L.). To date, only a few epigenetic modulators have been revealed to regulate wheat pow...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/7/2640 |
| Summary: | Powdery mildew disease caused by <i>Blumeria graminis</i> f.sp. <i>tritici</i> (<i>Bgt</i>) leads to severe economic losses in bread wheat (<i>Triticum aestivum</i> L.). To date, only a few epigenetic modulators have been revealed to regulate wheat powdery mildew resistance. In this study, the histone deacetylase 2 (HD2) type histone deacetylase TaHDT701 was identified as a negative regulator of wheat defense responses to <i>Bgt</i>. Using multiple approaches, we demonstrated that TaHDT701 associates with the RPD3 type histone deacetylase TaHDA6 and the WD40-repeat protein TaHOS15 to constitute a histone deacetylase complex, in which TaHDT701 could stabilize the TaHDA6-TaHOS15 association. Furthermore, knockdown of <i>TaHDT701</i>, <i>TaHDA6</i>, and <i>TaHOS15</i> resulted in enhanced wheat powdery mildew resistance, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex negatively regulates wheat defense responses to <i>Bgt</i>. Moreover, chromatin immunoprecipitation assays revealed that TaHDT701 could function in concert with TaHOS15 to recruit TaHDA6 to the promoters of defense-related genes such as <i>TaPR1</i>, <i>TaPR2</i>, <i>TaPR5</i>, and <i>TaWRKY45.</i> In addition, silencing of <i>TaHDT701</i>, <i>TaHDA6</i>, and <i>TaHOS15</i> resulted in the up-regulation of <i>TaPR1</i>, <i>TaPR2</i>, <i>TaPR5</i>, and <i>TaWRKY45</i> accompanied with increased histone acetylation and methylation, as well as reduced nucleosome occupancy, at their promoters, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex suppresses wheat powdery mildew resistance by modulating chromatin state at defense-related genes. |
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| ISSN: | 1661-6596 1422-0067 |