Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae)
<i>Eurytrema coelomaticum</i>, a pancreatic fluke, is recognized as a causative agent of substantial economic losses in ruminants. This infection, commonly referred to as eurytrematosis, is a significant concern due to its detrimental impact on livestock production. However, there is a p...
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MDPI AG
2023-12-01
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| Series: | Genes |
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| author | Fuqiang Huang Xin Li Bijin Ye Yule Zhou Zhisheng Dang Wenqiang Tang Long Wang Haoji Zhang Wenting Chui Jun Kui |
| author_facet | Fuqiang Huang Xin Li Bijin Ye Yule Zhou Zhisheng Dang Wenqiang Tang Long Wang Haoji Zhang Wenting Chui Jun Kui |
| author_sort | Fuqiang Huang |
| collection | DOAJ |
| description | <i>Eurytrema coelomaticum</i>, a pancreatic fluke, is recognized as a causative agent of substantial economic losses in ruminants. This infection, commonly referred to as eurytrematosis, is a significant concern due to its detrimental impact on livestock production. However, there is a paucity of knowledge regarding the mitochondrial genome of <i>E. coelomaticum</i>. In this study, we performed the initial sequencing of the complete mitochondrial genome of <i>E. coelomaticum</i>. Our findings unveiled that the mitochondrial genome of <i>E. coelomaticum</i> spans a length of 15,831 bp and consists of 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and two noncoding regions. The A+T content constituted 62.49% of the genome. Moreover, all 12 protein-coding genes of <i>E. coelomaticum</i> exhibit the same arrangement as those of <i>E. pancreaticum</i> and other published species belonging to the family Dicrocoeliidae. The presence of a short string of additional amino acids (approximately 20~23 aa) at the N-terminal of the cox1 protein in both <i>E. coelomaticum</i> and <i>E. pancreaticum</i> mitochondrial genomes has contributed to the elongation of the <i>cox1</i> gene in genus <i>Eurytrema</i>, surpassing that of all previously sequenced Dicrocoeliidae. The phylogenetic analysis displayed a close relationship between <i>E. coelomaticum</i> and <i>E. pancreaticum</i>, along with a genus-level association between <i>Eurytrema</i> and <i>Lyperosomum</i>. These findings underscore the importance of mitochondrial genomic data for comparative studies of Dicrocoeliidae and even Digenea, offering valuable DNA markers for future investigations in the systematic, epidemiological, and population genetic studies of this parasite and other digenean trematodes. |
| first_indexed | 2024-03-08T20:44:04Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-08T20:44:04Z |
| publishDate | 2023-12-01 |
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| series | Genes |
| spelling | doaj.art-ae19eba0ba2c4dfcab7bede410c405802023-12-22T14:11:16ZengMDPI AGGenes2073-44252023-12-011412219910.3390/genes14122199Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae)Fuqiang Huang0Xin Li1Bijin Ye2Yule Zhou3Zhisheng Dang4Wenqiang Tang5Long Wang6Haoji Zhang7Wenting Chui8Jun Kui9School of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaNational Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People’s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaState Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528225, ChinaAnimal Disease Prevention and Control Center of Qinghai Province, Xining 810003, ChinaHuangzhong District Animal Husbandry and Veterinary Station, Xining 811600, China<i>Eurytrema coelomaticum</i>, a pancreatic fluke, is recognized as a causative agent of substantial economic losses in ruminants. This infection, commonly referred to as eurytrematosis, is a significant concern due to its detrimental impact on livestock production. However, there is a paucity of knowledge regarding the mitochondrial genome of <i>E. coelomaticum</i>. In this study, we performed the initial sequencing of the complete mitochondrial genome of <i>E. coelomaticum</i>. Our findings unveiled that the mitochondrial genome of <i>E. coelomaticum</i> spans a length of 15,831 bp and consists of 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and two noncoding regions. The A+T content constituted 62.49% of the genome. Moreover, all 12 protein-coding genes of <i>E. coelomaticum</i> exhibit the same arrangement as those of <i>E. pancreaticum</i> and other published species belonging to the family Dicrocoeliidae. The presence of a short string of additional amino acids (approximately 20~23 aa) at the N-terminal of the cox1 protein in both <i>E. coelomaticum</i> and <i>E. pancreaticum</i> mitochondrial genomes has contributed to the elongation of the <i>cox1</i> gene in genus <i>Eurytrema</i>, surpassing that of all previously sequenced Dicrocoeliidae. The phylogenetic analysis displayed a close relationship between <i>E. coelomaticum</i> and <i>E. pancreaticum</i>, along with a genus-level association between <i>Eurytrema</i> and <i>Lyperosomum</i>. These findings underscore the importance of mitochondrial genomic data for comparative studies of Dicrocoeliidae and even Digenea, offering valuable DNA markers for future investigations in the systematic, epidemiological, and population genetic studies of this parasite and other digenean trematodes.https://www.mdpi.com/2073-4425/14/12/2199<i>Eurytrema coelomaticum</i>mitochondrial DNAphylogenetic analysisgenome |
| spellingShingle | Fuqiang Huang Xin Li Bijin Ye Yule Zhou Zhisheng Dang Wenqiang Tang Long Wang Haoji Zhang Wenting Chui Jun Kui Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae) Genes <i>Eurytrema coelomaticum</i> mitochondrial DNA phylogenetic analysis genome |
| title | Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae) |
| title_full | Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae) |
| title_fullStr | Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae) |
| title_full_unstemmed | Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae) |
| title_short | Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of <i>Eurytrema coelomaticum</i> (Trematoda: Dicrocoeliidae) |
| title_sort | characterization of the complete mitochondrial genome and phylogenetic analyses of i eurytrema coelomaticum i trematoda dicrocoeliidae |
| topic | <i>Eurytrema coelomaticum</i> mitochondrial DNA phylogenetic analysis genome |
| url | https://www.mdpi.com/2073-4425/14/12/2199 |
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