Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.)
IntroductionPutative mutants were generated through gamma irradiation in the polyembryonic mango genotype Nekkare. The putative mutant progenies along with control seedlings and mother plants were evaluated by comparing the compositions and relative proportions of their major volatile compounds.Meth...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1168947/full |
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| author | Nusrat Perveen M. R. Dinesh M. Sankaran K. S. Shivashankara K. V. Ravishankar R. Venugopal Hidayatullah Mir |
| author_facet | Nusrat Perveen M. R. Dinesh M. Sankaran K. S. Shivashankara K. V. Ravishankar R. Venugopal Hidayatullah Mir |
| author_sort | Nusrat Perveen |
| collection | DOAJ |
| description | IntroductionPutative mutants were generated through gamma irradiation in the polyembryonic mango genotype Nekkare. The putative mutant progenies along with control seedlings and mother plants were evaluated by comparing the compositions and relative proportions of their major volatile compounds.MethodsVolatile profiling was done using headspace-solid phase micro-extraction (HS SPME) method coupled with gas chromatography–mass spectrometry (GC-MS MS). Furthermore, characterisation of putative mutants and control seedlings was carried out using simple sequence repeat (SSR) markers to ascertain the genetic diversity present in the samples under study.ResultsMonoterpenes were the most abundant volatile compound in all the studied samples (ranging from 34.76% to 91.41%) out of which I-Phellandrene and cis-Ocimene formed the major fraction in mother plants (20.45%–21.86% and 16.17%–21.27%, respectively) and control seedlings (23.32%–24.95% and 18.95%–20.81%, respectively), while beta-Phellandrene was dominant in the selected putative mutant samples (2.34%–29.53%). Among sesquiterpenes, trans-Caryophyllene was detected only in the putative mutant samples (0.10%–30.18%). Grouping together of mother plants and control seedlings was seen in the cluster analysis, while the putative mutants grouped apart from them suggesting genetic diversity. Genetic distance between the mother plants and control seedlings ranged from 0.97 to 2.73, while between putative mutants, control seedlings, and mother plants, it ranged from 6.54 to 9.82. SSR-based characterisation of putative mutant seedlings showed that mutation caused variability in the treated population. This was evident from the high allelic richness ranging from 4 to 12 with a mean of 7 and a higher mean Shannon’s Information Index (1.50) of the putative mutant population.DiscussionThe study demonstrates that volatile profiling and molecular characterisation using SSR markers could be used as a tool to detect variation in a mutated population. In addition, volatile profiling can be used to validate putative mutants in polyembryonic mango genotypes where the seedlings of nucellar origin are similar to mother plants. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T11:27:30Z |
| publishDate | 2023-09-01 |
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| spelling | doaj.art-ae1c7af3018f447ca9c4eec513c784da2023-09-01T07:13:10ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.11689471168947Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.)Nusrat Perveen0M. R. Dinesh1M. Sankaran2K. S. Shivashankara3K. V. Ravishankar4R. Venugopal5Hidayatullah Mir6Division of Fruit Crops, Indian Council of Agricultural Research (ICAR)-Indian Institute of Horticultural Research, Bengaluru, IndiaDivision of Fruit Crops, Indian Council of Agricultural Research (ICAR)-Indian Institute of Horticultural Research, Bengaluru, IndiaDivision of Fruit Crops, Indian Council of Agricultural Research (ICAR)-Indian Institute of Horticultural Research, Bengaluru, IndiaDivision of Basic Sciences, Indian Council of Agricultural Research (ICAR)-Indian Institute of Horticultural Research, Bengaluru, IndiaDivision of Basic Sciences, Indian Council of Agricultural Research (ICAR)-Indian Institute of Horticultural Research, Bengaluru, IndiaDivision of Social Sciences and Training, Indian Council of Agricultural Research (ICAR)-Indian Institute of Horticultural Research, Bengaluru, IndiaDepartment of Horticulture (Fruit and Fruit Technology), Bihar Agricultural University, Bhagalpur, IndiaIntroductionPutative mutants were generated through gamma irradiation in the polyembryonic mango genotype Nekkare. The putative mutant progenies along with control seedlings and mother plants were evaluated by comparing the compositions and relative proportions of their major volatile compounds.MethodsVolatile profiling was done using headspace-solid phase micro-extraction (HS SPME) method coupled with gas chromatography–mass spectrometry (GC-MS MS). Furthermore, characterisation of putative mutants and control seedlings was carried out using simple sequence repeat (SSR) markers to ascertain the genetic diversity present in the samples under study.ResultsMonoterpenes were the most abundant volatile compound in all the studied samples (ranging from 34.76% to 91.41%) out of which I-Phellandrene and cis-Ocimene formed the major fraction in mother plants (20.45%–21.86% and 16.17%–21.27%, respectively) and control seedlings (23.32%–24.95% and 18.95%–20.81%, respectively), while beta-Phellandrene was dominant in the selected putative mutant samples (2.34%–29.53%). Among sesquiterpenes, trans-Caryophyllene was detected only in the putative mutant samples (0.10%–30.18%). Grouping together of mother plants and control seedlings was seen in the cluster analysis, while the putative mutants grouped apart from them suggesting genetic diversity. Genetic distance between the mother plants and control seedlings ranged from 0.97 to 2.73, while between putative mutants, control seedlings, and mother plants, it ranged from 6.54 to 9.82. SSR-based characterisation of putative mutant seedlings showed that mutation caused variability in the treated population. This was evident from the high allelic richness ranging from 4 to 12 with a mean of 7 and a higher mean Shannon’s Information Index (1.50) of the putative mutant population.DiscussionThe study demonstrates that volatile profiling and molecular characterisation using SSR markers could be used as a tool to detect variation in a mutated population. In addition, volatile profiling can be used to validate putative mutants in polyembryonic mango genotypes where the seedlings of nucellar origin are similar to mother plants.https://www.frontiersin.org/articles/10.3389/fpls.2023.1168947/fullmangopolyembryonymutationnucellar seedlingsSSR markersvolatile profiling |
| spellingShingle | Nusrat Perveen M. R. Dinesh M. Sankaran K. S. Shivashankara K. V. Ravishankar R. Venugopal Hidayatullah Mir Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.) Frontiers in Plant Science mango polyembryony mutation nucellar seedlings SSR markers volatile profiling |
| title | Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.) |
| title_full | Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.) |
| title_fullStr | Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.) |
| title_full_unstemmed | Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.) |
| title_short | Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.) |
| title_sort | volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango mangifera indica l |
| topic | mango polyembryony mutation nucellar seedlings SSR markers volatile profiling |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1168947/full |
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