Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae)
Ticks are specialized ectoparasites that feed on blood, causing physical harm to the host and facilitating pathogen transmission. The genus Haemaphysalis contains vectors for numerous infectious agents. These agents cause various diseases in humans and animals. Mitochondrial genome sequences serve a...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X24000049 |
| _version_ | 1797320980368982016 |
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| author | Wenqiang Tang Xin Li Bijin Ye Bin Shi Haoji Zhang Zhisheng Dang Yuexiang Sun Lamu Danqu Chenyang Xia Danzeng Quzhen Xialing Zhao Wenting Chui Fuqiang Huang |
| author_facet | Wenqiang Tang Xin Li Bijin Ye Bin Shi Haoji Zhang Zhisheng Dang Yuexiang Sun Lamu Danqu Chenyang Xia Danzeng Quzhen Xialing Zhao Wenting Chui Fuqiang Huang |
| author_sort | Wenqiang Tang |
| collection | DOAJ |
| description | Ticks are specialized ectoparasites that feed on blood, causing physical harm to the host and facilitating pathogen transmission. The genus Haemaphysalis contains vectors for numerous infectious agents. These agents cause various diseases in humans and animals. Mitochondrial genome sequences serve as reliable molecular markers, forming a crucial basis for evolutionary analyses, studying species origins, and exploring molecular phylogeny. We extracted mitochondrial genome from the enriched mitochondria of Haemaphysalis tibetensis and obtained a 14,714-bp sequence. The mitochondrial genome consists of 13 protein-coding genes (PCGs), two ribosomal RNA, 22 transfer RNAs (tRNAs), and two control regions. The nucleotide composition of H. tibetensis mitochondrial genome was 38.38 % for A, 9.61 % for G, 39.32 % for T, and 12.69 % for C. The A + T content of H. tibetensis mitochondrial genome was 77.7 %, significantly higher than the G + C content. The repeat units of H. tibetensis exhibited two identical repeat units of 33 bp in length, positioned downstream of nad1 and rrnL genes. Furthermore, phylogenetic analyses based on the 13 PCGs indicated that Haemaphysalis tibetensis (subgenus Allophysalis) formed a monophyletic clade with Haemaphysalis nepalensis (subgenus Herpetobia) and Haemaphysalis danieli (subgenus Allophysalis). Although the species Haemaphysalis inermis, Haemaphysalis kitaokai, Haemaphysalis kolonini, and Haemaphysalis colasbelcouri belong to the subgenus Alloceraea, which were morphologically primitive hemaphysalines just like H. tibetensis, these four tick species cannot form a single clade with H. tibetensis. In this study, the whole mitochondrial genome sequence of H. tibetensis from Tibet was obtained, which enriched the mitochondrial genome data of ticks and provided genetic markers to study the population heredity and molecular evolution of the genus Haemaphysalis. |
| first_indexed | 2024-03-08T04:50:55Z |
| format | Article |
| id | doaj.art-ae20dd6e153949288f9763d5b34658f6 |
| institution | Directory Open Access Journal |
| issn | 1877-9603 |
| language | English |
| last_indexed | 2024-03-08T04:50:55Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj.art-ae20dd6e153949288f9763d5b34658f62024-02-08T05:07:31ZengElsevierTicks and Tick-Borne Diseases1877-96032024-03-01152102311Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae)Wenqiang Tang0Xin Li1Bijin Ye2Bin Shi3Haoji Zhang4Zhisheng Dang5Yuexiang Sun6Lamu Danqu7Chenyang Xia8Danzeng Quzhen9Xialing Zhao10Wenting Chui11Fuqiang Huang12Institute of Animal Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Tibet Lhasa 850009, China; State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Lhasa 850002, ChinaSchool of Life Science and Engineering, Foshan University, Guangdong Foshan 528225, ChinaSchool of Life Science and Engineering, Foshan University, Guangdong Foshan 528225, ChinaInstitute of Animal Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Tibet Lhasa 850009, China; State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Lhasa 850002, ChinaSchool of Life Science and Engineering, Foshan University, Guangdong Foshan 528225, ChinaNational Institute of Parasitic Diseases at China CDC/Chinese Center for Tropical Diseases Research, WHO Collaborating Centre for Tropical Diseases, NHC Key Laboratory for Parasite and Vector Biology, Shanghai 200025, ChinaSchool of Life Science and Engineering, Foshan University, Guangdong Foshan 528225, ChinaInstitute of Animal Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Tibet Lhasa 850009, China; State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Lhasa 850002, ChinaInstitute of Animal Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Tibet Lhasa 850009, China; State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Lhasa 850002, ChinaInstitute of Animal Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Tibet Lhasa 850009, China; State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Lhasa 850002, ChinaInstitute of Animal Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Tibet Lhasa 850009, ChinaAnimal Disease Prevention and Control Center of Qinghai Province, ChinaSchool of Life Science and Engineering, Foshan University, Guangdong Foshan 528225, China; Corresponding author.Ticks are specialized ectoparasites that feed on blood, causing physical harm to the host and facilitating pathogen transmission. The genus Haemaphysalis contains vectors for numerous infectious agents. These agents cause various diseases in humans and animals. Mitochondrial genome sequences serve as reliable molecular markers, forming a crucial basis for evolutionary analyses, studying species origins, and exploring molecular phylogeny. We extracted mitochondrial genome from the enriched mitochondria of Haemaphysalis tibetensis and obtained a 14,714-bp sequence. The mitochondrial genome consists of 13 protein-coding genes (PCGs), two ribosomal RNA, 22 transfer RNAs (tRNAs), and two control regions. The nucleotide composition of H. tibetensis mitochondrial genome was 38.38 % for A, 9.61 % for G, 39.32 % for T, and 12.69 % for C. The A + T content of H. tibetensis mitochondrial genome was 77.7 %, significantly higher than the G + C content. The repeat units of H. tibetensis exhibited two identical repeat units of 33 bp in length, positioned downstream of nad1 and rrnL genes. Furthermore, phylogenetic analyses based on the 13 PCGs indicated that Haemaphysalis tibetensis (subgenus Allophysalis) formed a monophyletic clade with Haemaphysalis nepalensis (subgenus Herpetobia) and Haemaphysalis danieli (subgenus Allophysalis). Although the species Haemaphysalis inermis, Haemaphysalis kitaokai, Haemaphysalis kolonini, and Haemaphysalis colasbelcouri belong to the subgenus Alloceraea, which were morphologically primitive hemaphysalines just like H. tibetensis, these four tick species cannot form a single clade with H. tibetensis. In this study, the whole mitochondrial genome sequence of H. tibetensis from Tibet was obtained, which enriched the mitochondrial genome data of ticks and provided genetic markers to study the population heredity and molecular evolution of the genus Haemaphysalis.http://www.sciencedirect.com/science/article/pii/S1877959X24000049Haemaphysalis tibetensisRepeat unitsMitochondrial genomePhylogenetic treeHard ticks |
| spellingShingle | Wenqiang Tang Xin Li Bijin Ye Bin Shi Haoji Zhang Zhisheng Dang Yuexiang Sun Lamu Danqu Chenyang Xia Danzeng Quzhen Xialing Zhao Wenting Chui Fuqiang Huang Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae) Ticks and Tick-Borne Diseases Haemaphysalis tibetensis Repeat units Mitochondrial genome Phylogenetic tree Hard ticks |
| title | Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae) |
| title_full | Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae) |
| title_fullStr | Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae) |
| title_full_unstemmed | Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae) |
| title_short | Characterization of the complete mitochondrial genome and phylogenetic analyses of Haemaphysalis tibetensis Hoogstraal, 1965 (Acari: Ixodidae) |
| title_sort | characterization of the complete mitochondrial genome and phylogenetic analyses of haemaphysalis tibetensis hoogstraal 1965 acari ixodidae |
| topic | Haemaphysalis tibetensis Repeat units Mitochondrial genome Phylogenetic tree Hard ticks |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X24000049 |
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