| Summary: | CLE peptides are well-known hormonal regulators of plant development, but their role in somatic embryogenesis remains undetermined. <i>CLE</i> genes are often regulated by WOX transcription factors and, in their turn, regulate the expression level of <i>WOX</i> genes. In this study, we used in vitro cultivation, as well as qPCR and transcriptomic analysis, to find CLE peptides which could regulate the <i>MtWOX9-1</i> gene, stimulating somatic embryogenesis in <i>Medicago truncatula</i>. Three CLE peptides were found which could probably be such regulators, but none of them was found to influence <i>MtWOX9-1</i> expression in the embryogenic calli. Nevertheless, overexpression of one of <i>CLE</i> genes under study, <i>MtCLE16</i>, decreased somatic embryogenesis intensity. Additionally, overexpression of <i>MtCLE08</i> was found to suppress expression of <i>MtWOX13a</i>, a supposed antagonist of somatic embryo development. Our findings can be helpful for the search for new regeneration regulators which could be used for plant transformation.
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