Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate

Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate

Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport. By localizing the electric field over small portions of the cell memb...

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Bibliographic Details
Main Authors:	Tina Vindiš, Anja Blažič, Diaa Khayyat, Tjaša Potočnik, Shaurya Sachdev, Lea Rems
Format:	Article
Language:	English
Published:	MDPI AG 2022-09-01
Series:	Pharmaceutics
Subjects:	localized electroporation gene electrotransfer plasmid transfection cell culture insert numerical modeling
Online Access:	https://www.mdpi.com/1999-4923/14/9/1959

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