Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex
We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2017-02-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/6/2/210 |
| _version_ | 1818733760919633920 |
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| author | Apostolos-Manuel Koussoroplis Anke Schwarzenberger Alexander Wacker |
| author_facet | Apostolos-Manuel Koussoroplis Anke Schwarzenberger Alexander Wacker |
| author_sort | Apostolos-Manuel Koussoroplis |
| collection | DOAJ |
| description | We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of Synechococcus. When screening the Daphnia genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of D. pulex is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments. |
| first_indexed | 2024-12-17T23:54:36Z |
| format | Article |
| id | doaj.art-ae2539a34e57417292457f5e13e002b4 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T23:54:36Z |
| publishDate | 2017-02-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-ae2539a34e57417292457f5e13e002b42022-12-21T21:28:06ZengThe Company of BiologistsBiology Open2046-63902017-02-016221021610.1242/bio.022046022046Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulexApostolos-Manuel Koussoroplis0Anke Schwarzenberger1Alexander Wacker2 Theoretical Aquatic Ecology an Ecophysiology Group, Institute for Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany Theoretical Aquatic Ecology an Ecophysiology Group, Institute for Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany Theoretical Aquatic Ecology an Ecophysiology Group, Institute for Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of Synechococcus. When screening the Daphnia genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of D. pulex is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments.http://bio.biologists.org/content/6/2/210CyanobacteriaDigestive enzyme activityNutritional qualityLipases |
| spellingShingle | Apostolos-Manuel Koussoroplis Anke Schwarzenberger Alexander Wacker Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex Biology Open Cyanobacteria Digestive enzyme activity Nutritional quality Lipases |
| title | Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex |
| title_full | Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex |
| title_fullStr | Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex |
| title_full_unstemmed | Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex |
| title_short | Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex |
| title_sort | diet quality determines lipase gene expression and lipase esterase activity in daphnia pulex |
| topic | Cyanobacteria Digestive enzyme activity Nutritional quality Lipases |
| url | http://bio.biologists.org/content/6/2/210 |
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