Resistance of Abaca Somaclonal Variant Against Fusarium
The objectives of this study were (i) to evaluate responses against F. oxysporum f.sp. cubense (Foc) infection of abaca variants regenerated using four different methods, (ii) to determine initial root length and plant height effects on survival of inoculated abaca variants, and (iii) to identify Fo...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2007-12-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916303655 |
| _version_ | 1811238140304687104 |
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| author | RULLY DYAH PURWATI SUDARSONO |
| author_facet | RULLY DYAH PURWATI SUDARSONO |
| author_sort | RULLY DYAH PURWATI |
| collection | DOAJ |
| description | The objectives of this study were (i) to evaluate responses against F. oxysporum f.sp. cubense (Foc) infection of abaca variants regenerated using four different methods, (ii) to determine initial root length and plant height effects on survival of inoculated abaca variants, and (iii) to identify Foc resistance abaca variants. In the previous experiment, four abaca variant lines were regenerated from (i) embryogenic calli (TC line), (ii) ethyl methyl sulphonate (EMS) treated embryogenic calli (EMS line), (iii) EMS treated embryogenic calli, followed by in vitro selection on Foc culture filtrate (EMS+CF line), and (iv) EMS treated embryogenic calli, followed by in vitro selection on fusaric acid (EMS+FA line). All abaca variants were grown in a glasshouse and inoculated with Banyuwangi isolate of Foc (Foc Bw). Initial root length (RL) and plant height (PH) of the abaca variants were recorded before inoculation, while scores of plant damage (SPD), and their survival were recorded at 60 days after inoculation (DAI). The results showed that the initial RL and PH did not affect survival of the tested abaca variants. Regardless of their initial RL and PH, susceptible abaca variants died before 60 DAI while resistance ones still survived. Abaca variants regenerated from single clump of embryogenic callus showed an array of responses against Foc Bw infection, indicating the existence of a mix cells population. The Foc Bw resistance abaca variants were successfully identified from four tested abaca variant lines, although with different frequencies. However, more Foc Bw resistance abaca plants were identified from EMS+CF line than the others. Using the developed procedures, 8 resistance abaca plants were identified from abaca cv. Tangongon and 12 from abaca cv. Sangihe-1. |
| first_indexed | 2024-04-12T12:35:18Z |
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| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-04-12T12:35:18Z |
| publishDate | 2007-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-ae26e4d223e64c749890e8b8ba75576f2022-12-22T03:32:54ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192007-12-0114413313910.4308/hjb.14.4.133Resistance of Abaca Somaclonal Variant Against FusariumRULLY DYAH PURWATI0SUDARSONO1Research Institute for Tobacco and Fibre Crops, Jalan Raya Karangploso, P.O. Box 199, Malang 65000, IndonesiaPMB Lab, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaThe objectives of this study were (i) to evaluate responses against F. oxysporum f.sp. cubense (Foc) infection of abaca variants regenerated using four different methods, (ii) to determine initial root length and plant height effects on survival of inoculated abaca variants, and (iii) to identify Foc resistance abaca variants. In the previous experiment, four abaca variant lines were regenerated from (i) embryogenic calli (TC line), (ii) ethyl methyl sulphonate (EMS) treated embryogenic calli (EMS line), (iii) EMS treated embryogenic calli, followed by in vitro selection on Foc culture filtrate (EMS+CF line), and (iv) EMS treated embryogenic calli, followed by in vitro selection on fusaric acid (EMS+FA line). All abaca variants were grown in a glasshouse and inoculated with Banyuwangi isolate of Foc (Foc Bw). Initial root length (RL) and plant height (PH) of the abaca variants were recorded before inoculation, while scores of plant damage (SPD), and their survival were recorded at 60 days after inoculation (DAI). The results showed that the initial RL and PH did not affect survival of the tested abaca variants. Regardless of their initial RL and PH, susceptible abaca variants died before 60 DAI while resistance ones still survived. Abaca variants regenerated from single clump of embryogenic callus showed an array of responses against Foc Bw infection, indicating the existence of a mix cells population. The Foc Bw resistance abaca variants were successfully identified from four tested abaca variant lines, although with different frequencies. However, more Foc Bw resistance abaca plants were identified from EMS+CF line than the others. Using the developed procedures, 8 resistance abaca plants were identified from abaca cv. Tangongon and 12 from abaca cv. Sangihe-1.http://www.sciencedirect.com/science/article/pii/S1978301916303655Fusarium wilts resistancein vitro selectionculture filtratefusaric acidEMS |
| spellingShingle | RULLY DYAH PURWATI SUDARSONO Resistance of Abaca Somaclonal Variant Against Fusarium Hayati Journal of Biosciences Fusarium wilts resistance in vitro selection culture filtrate fusaric acid EMS |
| title | Resistance of Abaca Somaclonal Variant Against Fusarium |
| title_full | Resistance of Abaca Somaclonal Variant Against Fusarium |
| title_fullStr | Resistance of Abaca Somaclonal Variant Against Fusarium |
| title_full_unstemmed | Resistance of Abaca Somaclonal Variant Against Fusarium |
| title_short | Resistance of Abaca Somaclonal Variant Against Fusarium |
| title_sort | resistance of abaca somaclonal variant against fusarium |
| topic | Fusarium wilts resistance in vitro selection culture filtrate fusaric acid EMS |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916303655 |
| work_keys_str_mv | AT rullydyahpurwati resistanceofabacasomaclonalvariantagainstfusarium AT sudarsono resistanceofabacasomaclonalvariantagainstfusarium |