Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs
Escherichia coli is an important component of the normal bacterial community in humans and animals. However, in recent years, the pathogenicity of some virulent strains to the outside of the intestine has been confirmed in clinical medicine. The purpose of this study was to analyze the effect of cya...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3056 |
| _version_ | 1797356219981103104 |
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| author | Yingjin CHAI Xiaoxiao GU Yingni SUN Jie LI Xiaolan WANG Xin HUANG Xia ZHOU Mengli HAN Fagang ZHONG Xingxing ZHANG Tongzhong WU |
| author_facet | Yingjin CHAI Xiaoxiao GU Yingni SUN Jie LI Xiaolan WANG Xin HUANG Xia ZHOU Mengli HAN Fagang ZHONG Xingxing ZHANG Tongzhong WU |
| author_sort | Yingjin CHAI |
| collection | DOAJ |
| description | Escherichia coli is an important component of the normal bacterial community in
humans and animals. However, in recent years, the pathogenicity of some virulent
strains to the outside of the intestine has been confirmed in clinical medicine. The
purpose of this study was to analyze the effect of cyaA gene knockout on the biological
characteristics and pathogenicity of extraintestinal pathogenic E. coli. In this study,
ExPEC strain (XJ10) was isolated from the lung tissue of sheep with respiratory tract
infection, and the mutation strain and complementary strain of cyaA gene were
constructed. Real-time PCR analysis showed that expression levels of most genes related
to carbon source utilization and adhesion invasion were down-regulated in the cyaA
mutant compared to the wild type; Quantification of the number of colonies showed
that cyaA mutation results in the number of adhesion and invasive to TC-1 cells colonies
decreased compared with wild type; Finally, as the cyaA gene mutated, the value of the
LD50 increased as compared to wild type, based on the LD50 calculation. Taken together,
mutations in the cyaA gene reduce growth rates, resulting in down-regulation of the
transcription levels of genes associated with carbon source utilization and adhesion
invasion, and a marked reduction in virulence. |
| first_indexed | 2024-03-08T14:23:27Z |
| format | Article |
| id | doaj.art-ae2790cd504b4aeba0a8c25fd5609f1e |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-08T14:23:27Z |
| publishDate | 2023-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-ae2790cd504b4aeba0a8c25fd5609f1e2024-01-13T17:59:33ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-12-01301637110.9775/kvfd.2023.304183056Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungsYingjin CHAI0Xiaoxiao GU1Yingni SUN2Jie LI3Xiaolan WANG4Xin HUANG5Xia ZHOU6Mengli HAN7Fagang ZHONG8Xingxing ZHANG9Tongzhong WU10State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, CHINADepartment of Architecture, Ningbo Nottingham University, Ningbo 315199, Zhejiang, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, CHINAState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, CHINAState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang, CHINAState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang, CHINAState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang, CHINAState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang, CHINAEscherichia coli is an important component of the normal bacterial community in humans and animals. However, in recent years, the pathogenicity of some virulent strains to the outside of the intestine has been confirmed in clinical medicine. The purpose of this study was to analyze the effect of cyaA gene knockout on the biological characteristics and pathogenicity of extraintestinal pathogenic E. coli. In this study, ExPEC strain (XJ10) was isolated from the lung tissue of sheep with respiratory tract infection, and the mutation strain and complementary strain of cyaA gene were constructed. Real-time PCR analysis showed that expression levels of most genes related to carbon source utilization and adhesion invasion were down-regulated in the cyaA mutant compared to the wild type; Quantification of the number of colonies showed that cyaA mutation results in the number of adhesion and invasive to TC-1 cells colonies decreased compared with wild type; Finally, as the cyaA gene mutated, the value of the LD50 increased as compared to wild type, based on the LD50 calculation. Taken together, mutations in the cyaA gene reduce growth rates, resulting in down-regulation of the transcription levels of genes associated with carbon source utilization and adhesion invasion, and a marked reduction in virulence.https://vetdergikafkas.org/pdf.php?id=3056adhesion and invasioncyaamedian lethal dosexj10 |
| spellingShingle | Yingjin CHAI Xiaoxiao GU Yingni SUN Jie LI Xiaolan WANG Xin HUANG Xia ZHOU Mengli HAN Fagang ZHONG Xingxing ZHANG Tongzhong WU Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs Kafkas Universitesi Veteriner Fakültesi Dergisi adhesion and invasion cyaa median lethal dose xj10 |
| title | Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs |
| title_full | Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs |
| title_fullStr | Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs |
| title_full_unstemmed | Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs |
| title_short | Adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs |
| title_sort | adenylate cyclase affects the virulence of extraintestinal pathogenic escherichia coli derived from sheep lungs |
| topic | adhesion and invasion cyaa median lethal dose xj10 |
| url | https://vetdergikafkas.org/pdf.php?id=3056 |
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