Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis
AtDeg2 is a chloroplast protein with dual protease/chaperone activity. Since data on how the individual activities of AtDeg2 affect growth and development of Arabidopsis thaliana plants is missing, two transgenic lines were prepared that express mutated AtDeg2 versions that have either only protease...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2018-06-01
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| Series: | Acta Societatis Botanicorum Poloniae |
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| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/8193 |
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| author | Małgorzata Adamiec Przemysław Jagodzik Tomasz P. Wyka Agnieszka Ludwików Filip Mituła Lucyna Misztal Robert Luciński Grzegorz Jackowski |
| author_facet | Małgorzata Adamiec Przemysław Jagodzik Tomasz P. Wyka Agnieszka Ludwików Filip Mituła Lucyna Misztal Robert Luciński Grzegorz Jackowski |
| author_sort | Małgorzata Adamiec |
| collection | DOAJ |
| description | AtDeg2 is a chloroplast protein with dual protease/chaperone activity. Since data on how the individual activities of AtDeg2 affect growth and development of Arabidopsis thaliana plants is missing, two transgenic lines were prepared that express mutated AtDeg2 versions that have either only protease or chaperone activity and a comprehensive ontogenesis stage-based study was performed comprising wild type (WT) plants and insertional mutants that do not express AtDeg2, as well as the two transgenic lines. The repression of both AtDeg2 activities in deg2-3 mutants altered just a few phenotypic traits including the time when cotyledons were fully opened, the time when 10% flowers were open as well as the number of inflorescence branches and seed length in plants which have completed their generative development. It was demonstrated that complete opening of cotyledons as well as the number of inflorescence branches and seed length in plants which have completed their generative development required involvement of both AtDeg2 activities, whereas the time when 10% of flowers were open was controlled by AtDeg2 protease activity. These results show for the first time that the chaperone activity of AtDeg2 is needed for some elements of generative development of A. thaliana plants to proceed normally. So far, the chaperone activity of AtDeg2 was confirmed based on in vitro assays only. |
| first_indexed | 2024-12-12T12:52:06Z |
| format | Article |
| id | doaj.art-ae285f146308412893e5f818f0f329ae |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T12:52:06Z |
| publishDate | 2018-06-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-ae285f146308412893e5f818f0f329ae2022-12-22T00:23:58ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802018-06-0187210.5586/asbp.35846889Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in ArabidopsisMałgorzata Adamiec0Przemysław Jagodzik1Tomasz P. Wyka2Agnieszka Ludwików3Filip Mituła4Lucyna Misztal5Robert Luciński6Grzegorz Jackowski7Department of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of General Botany, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of Biotechnology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of Biotechnology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańDepartment of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Umultowska 89, 61-614 PoznańAtDeg2 is a chloroplast protein with dual protease/chaperone activity. Since data on how the individual activities of AtDeg2 affect growth and development of Arabidopsis thaliana plants is missing, two transgenic lines were prepared that express mutated AtDeg2 versions that have either only protease or chaperone activity and a comprehensive ontogenesis stage-based study was performed comprising wild type (WT) plants and insertional mutants that do not express AtDeg2, as well as the two transgenic lines. The repression of both AtDeg2 activities in deg2-3 mutants altered just a few phenotypic traits including the time when cotyledons were fully opened, the time when 10% flowers were open as well as the number of inflorescence branches and seed length in plants which have completed their generative development. It was demonstrated that complete opening of cotyledons as well as the number of inflorescence branches and seed length in plants which have completed their generative development required involvement of both AtDeg2 activities, whereas the time when 10% of flowers were open was controlled by AtDeg2 protease activity. These results show for the first time that the chaperone activity of AtDeg2 is needed for some elements of generative development of A. thaliana plants to proceed normally. So far, the chaperone activity of AtDeg2 was confirmed based on in vitro assays only.https://pbsociety.org.pl/journals/index.php/asbp/article/view/8193AtDeg2 chloroplast proteinchaperoneleafmutantontogenesisphotosynthetic rateproteasetransformant |
| spellingShingle | Małgorzata Adamiec Przemysław Jagodzik Tomasz P. Wyka Agnieszka Ludwików Filip Mituła Lucyna Misztal Robert Luciński Grzegorz Jackowski Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis Acta Societatis Botanicorum Poloniae AtDeg2 chloroplast protein chaperone leaf mutant ontogenesis photosynthetic rate protease transformant |
| title | Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis |
| title_full | Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis |
| title_fullStr | Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis |
| title_full_unstemmed | Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis |
| title_short | Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis |
| title_sort | chloroplast protease chaperone atdeg2 influences cotyledons opening and reproductive development in arabidopsis |
| topic | AtDeg2 chloroplast protein chaperone leaf mutant ontogenesis photosynthetic rate protease transformant |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/8193 |
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