Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina
OBJECTIVES: High incidence and case fatality of unstable angina (UA) is, to a large extent, a consequence of the lack of highly sensitive and specific non-invasive markers. Circulating microRNAs (miRNAs) have been widely recommended as potential biomarkers for numerous diseases. In the present study...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier España
2020-07-01
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| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322020000100245&tlng=en |
| _version_ | 1811318146866348032 |
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| author | Sudong Liu Xuemin Guo Wei Zhong Ruiqiang Weng Jing Liu Xiaodong Gu Zhixiong Zhong |
| author_facet | Sudong Liu Xuemin Guo Wei Zhong Ruiqiang Weng Jing Liu Xiaodong Gu Zhixiong Zhong |
| author_sort | Sudong Liu |
| collection | DOAJ |
| description | OBJECTIVES: High incidence and case fatality of unstable angina (UA) is, to a large extent, a consequence of the lack of highly sensitive and specific non-invasive markers. Circulating microRNAs (miRNAs) have been widely recommended as potential biomarkers for numerous diseases. In the present study, we characterized distinctive miRNA expression profiles in patients with stable angina (SA), UA, and normal coronary arteries (NCA), and identified promising candidates for UA diagnosis. METHODS: Serum was collected from patients with SA, UA, and NCA who visited the Department of Cardiovascular Diseases of the Meizhou People’s Hospital. Small RNA sequencing was carried out on an Illumina HiSeq 2500 platform. miRNA expression in different groups of patients was profiled and then confirmed based on that in an independent set of patients. Functions of differentially expressed miRNAs were predicted using gene ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis. RESULTS: Our results indicated that circulating miRNA expression profiles differed between SA, UA, and NCA patients. A total of 36 and 161 miRNAs were dysregulated in SA and UA patients, respectively. miRNA expression was validated by reverse transcription quantitative polymerase chain reaction. CONCLUSION: The results suggest that circulating miRNAs are potential biomarkers of UA. |
| first_indexed | 2024-04-13T12:20:49Z |
| format | Article |
| id | doaj.art-ae29f356409d484b898ec2d4bb9458ed |
| institution | Directory Open Access Journal |
| issn | 1980-5322 |
| language | English |
| last_indexed | 2024-04-13T12:20:49Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-ae29f356409d484b898ec2d4bb9458ed2022-12-22T02:47:14ZengElsevier EspañaClinics1980-53222020-07-017510.6061/clinics/2020/e1546Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable AnginaSudong LiuXuemin GuoWei ZhongRuiqiang WengJing LiuXiaodong GuZhixiong Zhonghttps://orcid.org/0000-0002-3200-3105OBJECTIVES: High incidence and case fatality of unstable angina (UA) is, to a large extent, a consequence of the lack of highly sensitive and specific non-invasive markers. Circulating microRNAs (miRNAs) have been widely recommended as potential biomarkers for numerous diseases. In the present study, we characterized distinctive miRNA expression profiles in patients with stable angina (SA), UA, and normal coronary arteries (NCA), and identified promising candidates for UA diagnosis. METHODS: Serum was collected from patients with SA, UA, and NCA who visited the Department of Cardiovascular Diseases of the Meizhou People’s Hospital. Small RNA sequencing was carried out on an Illumina HiSeq 2500 platform. miRNA expression in different groups of patients was profiled and then confirmed based on that in an independent set of patients. Functions of differentially expressed miRNAs were predicted using gene ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis. RESULTS: Our results indicated that circulating miRNA expression profiles differed between SA, UA, and NCA patients. A total of 36 and 161 miRNAs were dysregulated in SA and UA patients, respectively. miRNA expression was validated by reverse transcription quantitative polymerase chain reaction. CONCLUSION: The results suggest that circulating miRNAs are potential biomarkers of UA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322020000100245&tlng=enCoronary Artery DiseaseMicroRNA (miRNA)Stable AnginaUnstable AnginaRNA sequencing (RNA-seq) |
| spellingShingle | Sudong Liu Xuemin Guo Wei Zhong Ruiqiang Weng Jing Liu Xiaodong Gu Zhixiong Zhong Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina Clinics Coronary Artery Disease MicroRNA (miRNA) Stable Angina Unstable Angina RNA sequencing (RNA-seq) |
| title | Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina |
| title_full | Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina |
| title_fullStr | Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina |
| title_full_unstemmed | Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina |
| title_short | Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina |
| title_sort | circulating microrna expression profiles in patients with stable and unstable angina |
| topic | Coronary Artery Disease MicroRNA (miRNA) Stable Angina Unstable Angina RNA sequencing (RNA-seq) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322020000100245&tlng=en |
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