Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis
The effects of oleic acid addition methods on the metabolic flux distribution of ganoderic acids R, S and T’s biosynthesis from <i>Ganoderma lucidum</i> were investigated. The results showed that adding filter-sterilized oleic acid in the process of submerged fermentation and static cult...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/6/615 |
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| author | Meng-Qiu Yan Xiao-Wei Su Yan-Fang Liu Chuan-Hong Tang Qing-Jiu Tang Shuai Zhou Yi Tan Li-Ping Liu Jing-Song Zhang Jie Feng |
| author_facet | Meng-Qiu Yan Xiao-Wei Su Yan-Fang Liu Chuan-Hong Tang Qing-Jiu Tang Shuai Zhou Yi Tan Li-Ping Liu Jing-Song Zhang Jie Feng |
| author_sort | Meng-Qiu Yan |
| collection | DOAJ |
| description | The effects of oleic acid addition methods on the metabolic flux distribution of ganoderic acids R, S and T’s biosynthesis from <i>Ganoderma lucidum</i> were investigated. The results showed that adding filter-sterilized oleic acid in the process of submerged fermentation and static culture is of benefit to the synthesis of ganoderic acids R, S and T. The metabolic fluxes were increased by 97.48%, 78.42% and 43.39%, respectively. The content of ganoderic acids R, S and T were 3.11 times, 5.19 times and 1.44 times higher, respectively, than they were in the control group, which was without additional oleic acid. Ganoderic acids R, S and T’s synthesis pathways (GAP), tricarboxylic acid cycles (TCA), pentose phosphate pathways (PP) and glycolysis pathways (EMP) were all enhanced in the process. Therefore, additional oleic acid can strengthen the overall metabolic flux distribution of <i>G. lucidum</i> in a submerged fermentation-static culture and it can reduce the accumulation of the by-product mycosterol. This study has laid an important foundation for improving the production of triterpenes in the submerged fermentation of <i>G. lucidum</i>. |
| first_indexed | 2024-03-09T23:22:44Z |
| format | Article |
| id | doaj.art-ae2de9fe2cae4cad8bfb6b0dc78f7f64 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T23:22:44Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-ae2de9fe2cae4cad8bfb6b0dc78f7f642023-11-23T17:25:02ZengMDPI AGJournal of Fungi2309-608X2022-06-018661510.3390/jof8060615Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s BiosynthesisMeng-Qiu Yan0Xiao-Wei Su1Yan-Fang Liu2Chuan-Hong Tang3Qing-Jiu Tang4Shuai Zhou5Yi Tan6Li-Ping Liu7Jing-Song Zhang8Jie Feng9Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, ChinaThe effects of oleic acid addition methods on the metabolic flux distribution of ganoderic acids R, S and T’s biosynthesis from <i>Ganoderma lucidum</i> were investigated. The results showed that adding filter-sterilized oleic acid in the process of submerged fermentation and static culture is of benefit to the synthesis of ganoderic acids R, S and T. The metabolic fluxes were increased by 97.48%, 78.42% and 43.39%, respectively. The content of ganoderic acids R, S and T were 3.11 times, 5.19 times and 1.44 times higher, respectively, than they were in the control group, which was without additional oleic acid. Ganoderic acids R, S and T’s synthesis pathways (GAP), tricarboxylic acid cycles (TCA), pentose phosphate pathways (PP) and glycolysis pathways (EMP) were all enhanced in the process. Therefore, additional oleic acid can strengthen the overall metabolic flux distribution of <i>G. lucidum</i> in a submerged fermentation-static culture and it can reduce the accumulation of the by-product mycosterol. This study has laid an important foundation for improving the production of triterpenes in the submerged fermentation of <i>G. lucidum</i>.https://www.mdpi.com/2309-608X/8/6/615<i>Ganoderma lucidum</i>submerged fermentation-static cultureoleic acidmetabolic flux analysisganoderic acid |
| spellingShingle | Meng-Qiu Yan Xiao-Wei Su Yan-Fang Liu Chuan-Hong Tang Qing-Jiu Tang Shuai Zhou Yi Tan Li-Ping Liu Jing-Song Zhang Jie Feng Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis Journal of Fungi <i>Ganoderma lucidum</i> submerged fermentation-static culture oleic acid metabolic flux analysis ganoderic acid |
| title | Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis |
| title_full | Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis |
| title_fullStr | Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis |
| title_full_unstemmed | Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis |
| title_short | Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis |
| title_sort | effects of oleic acid addition methods on the metabolic flux distribution of ganoderic acids r s and t s biosynthesis |
| topic | <i>Ganoderma lucidum</i> submerged fermentation-static culture oleic acid metabolic flux analysis ganoderic acid |
| url | https://www.mdpi.com/2309-608X/8/6/615 |
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