AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET

Adulteration in patchouli oil trade is an ancient practice, where additional substances like turpentine oil are added to increase volume and odor. Therefore, this research aimed to determine the composition of patchouli oil, considering variations in growing regions and potential adulteration in th...

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Main Authors: Laela Hayu Nurani, Any Guntarti, Rida Dwi Milanie, Ibnu Gholib Gandjar, Ichwan Ridwan Rais, Dian Prasasti, Citra Ariani Edityaningrum
Format: Article
Language:Indonesian
Published: Universitas Muhammadiyah Magelang 2024-04-01
Series:Jurnal Farmasi Sains dan Praktis
Subjects:
Online Access:https://journal.unimma.ac.id/index.php/pharmacy/article/view/10523
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author Laela Hayu Nurani
Any Guntarti
Rida Dwi Milanie
Ibnu Gholib Gandjar
Ichwan Ridwan Rais
Dian Prasasti
Citra Ariani Edityaningrum
author_facet Laela Hayu Nurani
Any Guntarti
Rida Dwi Milanie
Ibnu Gholib Gandjar
Ichwan Ridwan Rais
Dian Prasasti
Citra Ariani Edityaningrum
author_sort Laela Hayu Nurani
collection DOAJ
description Adulteration in patchouli oil trade is an ancient practice, where additional substances like turpentine oil are added to increase volume and odor. Therefore, this research aimed to determine the composition of patchouli oil, considering variations in growing regions and potential adulteration in the products available in the market. The analysis was carried out using the Gas Chromatography-Mass Spectrormetry (GC-MS) method with a Principal Component Analysis (PCA) chemometric combination for authentication. The sample obtained from Bantul, Kulon Progo, and Purworejo was isolated through steam and water distillation. Subsequently, it was tested in accordance with SNI standard 06-2385-2006 and analyzed using GC-MS. The results were compared to the products on the market and processed using multivariate chemometric, namely PCA with Minitab 19 software. GC-MS analysis showed the presence of various compounds contained in the oil, including alcohol, alpha-guaiene, beta-humulene, seychellene, trans-caryophyllene, neoalloocimene, and beta-patchoulene. The combination of GC-MS and PCA chemometrics was able to distinguish patchouli oil from others and the products available on the market. PCA chemometric analysis showed that the patchouli oil from various growing regions had the same chemical components as essential oil. Furthermore, PCA chemometric analysis of market products also showed similar results but varied significantly from turpentine oil. This showed that the patchouli oil product available on the market did not contain turpentine oil.
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spelling doaj.art-ae2ecf4f745b488a9ffe8bde357b64332024-04-19T05:38:15ZindUniversitas Muhammadiyah MagelangJurnal Farmasi Sains dan Praktis2549-90682579-45582024-04-0110110.31603/pharmacy.v10i1.10523AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKETLaela Hayu Nurani0Any Guntarti1Rida Dwi Milanie2Ibnu Gholib Gandjar3Ichwan Ridwan Rais4Dian Prasasti5Citra Ariani Edityaningrum6Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, IndonesiaFaculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, IndonesiaFaculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, IndonesiaFaculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, IndonesiaFaculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, IndonesiaFaculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, IndonesiaFaculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, Indonesia Adulteration in patchouli oil trade is an ancient practice, where additional substances like turpentine oil are added to increase volume and odor. Therefore, this research aimed to determine the composition of patchouli oil, considering variations in growing regions and potential adulteration in the products available in the market. The analysis was carried out using the Gas Chromatography-Mass Spectrormetry (GC-MS) method with a Principal Component Analysis (PCA) chemometric combination for authentication. The sample obtained from Bantul, Kulon Progo, and Purworejo was isolated through steam and water distillation. Subsequently, it was tested in accordance with SNI standard 06-2385-2006 and analyzed using GC-MS. The results were compared to the products on the market and processed using multivariate chemometric, namely PCA with Minitab 19 software. GC-MS analysis showed the presence of various compounds contained in the oil, including alcohol, alpha-guaiene, beta-humulene, seychellene, trans-caryophyllene, neoalloocimene, and beta-patchoulene. The combination of GC-MS and PCA chemometrics was able to distinguish patchouli oil from others and the products available on the market. PCA chemometric analysis showed that the patchouli oil from various growing regions had the same chemical components as essential oil. Furthermore, PCA chemometric analysis of market products also showed similar results but varied significantly from turpentine oil. This showed that the patchouli oil product available on the market did not contain turpentine oil. https://journal.unimma.ac.id/index.php/pharmacy/article/view/10523AuthenticationEssential oilGC-MSPatchouli oilPCA chemometrics
spellingShingle Laela Hayu Nurani
Any Guntarti
Rida Dwi Milanie
Ibnu Gholib Gandjar
Ichwan Ridwan Rais
Dian Prasasti
Citra Ariani Edityaningrum
AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET
Jurnal Farmasi Sains dan Praktis
Authentication
Essential oil
GC-MS
Patchouli oil
PCA chemometrics
title AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET
title_full AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET
title_fullStr AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET
title_full_unstemmed AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET
title_short AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET
title_sort authentication of patchouli oil from various growing regions using gc ms method with chemometric combination on the products in the market
topic Authentication
Essential oil
GC-MS
Patchouli oil
PCA chemometrics
url https://journal.unimma.ac.id/index.php/pharmacy/article/view/10523
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AT ridadwimilanie authenticationofpatchoulioilfromvariousgrowingregionsusinggcmsmethodwithchemometriccombinationontheproductsinthemarket
AT ibnugholibgandjar authenticationofpatchoulioilfromvariousgrowingregionsusinggcmsmethodwithchemometriccombinationontheproductsinthemarket
AT ichwanridwanrais authenticationofpatchoulioilfromvariousgrowingregionsusinggcmsmethodwithchemometriccombinationontheproductsinthemarket
AT dianprasasti authenticationofpatchoulioilfromvariousgrowingregionsusinggcmsmethodwithchemometriccombinationontheproductsinthemarket
AT citraarianiedityaningrum authenticationofpatchoulioilfromvariousgrowingregionsusinggcmsmethodwithchemometriccombinationontheproductsinthemarket