Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity
Tellurite [Te(IV)] is a high-toxicity metalloid. In this study, a fungus with high Te(IV) resistance was isolated. Strain AB1 could efficiently reduce highly toxic Te(IV) to less toxic Te(0). The reduced products formed rod-shaped biogenetic Te(0) nanoparticles (Bio-TeNPs) intracellularly. Further T...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1020179/full |
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| author | Bo Ao Fei He Jing Lv Junming Tu Zheng Tan Honglin Jiang Xiaoshan Shi Jingjing Li Jingjing Li Jianjun Hou Yuanliang Hu Xian Xia |
| author_facet | Bo Ao Fei He Jing Lv Junming Tu Zheng Tan Honglin Jiang Xiaoshan Shi Jingjing Li Jingjing Li Jianjun Hou Yuanliang Hu Xian Xia |
| author_sort | Bo Ao |
| collection | DOAJ |
| description | Tellurite [Te(IV)] is a high-toxicity metalloid. In this study, a fungus with high Te(IV) resistance was isolated. Strain AB1 could efficiently reduce highly toxic Te(IV) to less toxic Te(0). The reduced products formed rod-shaped biogenetic Te(0) nanoparticles (Bio-TeNPs) intracellularly. Further TEM-element mapping, FTIR, and XPS analysis showed that the extracted Bio-TeNPs ranged from 100 to 500 nm and consisted of Te(0), proteins, lipids, aromatic compounds, and carbohydrates. Moreover, Bio-TeNPs exhibited excellent antibacterial ability against Shigella dysenteriae, Escherichia coli, Enterobacter sakazakii, and Salmonella typhimurium according to inhibition zone tests. Further growth and live/dead staining experiments showed that E. coli and S. typhimurium were significantly inhibited by Bio-TeNPs, and cells were broken or shriveled after treatment with Bio-TeNPs based on SEM observation. Additionally, the antioxidant and cytotoxicity tests showed that the Bio-TeNPs exhibited excellent antioxidant capacity with no cytotoxicity. All these results suggested that strain AB1 showed great potential in bioremediation and Bio-TeNPs were excellent antibacterial nanomaterials with no cytotoxicity. |
| first_indexed | 2024-04-12T14:00:04Z |
| format | Article |
| id | doaj.art-ae2fe77eeddf4f1fa4ca878d580ddcbc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T14:00:04Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ae2fe77eeddf4f1fa4ca878d580ddcbc2022-12-22T03:30:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10201791020179Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activityBo Ao0Fei He1Jing Lv2Junming Tu3Zheng Tan4Honglin Jiang5Xiaoshan Shi6Jingjing Li7Jingjing Li8Jianjun Hou9Yuanliang Hu10Xian Xia11Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Provincial Center for Disease Control and Prevention, Wuhan, ChinaHubei Provincial Center for Disease Control and Prevention, Wuhan, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Provincial Center for Disease Control and Prevention, Wuhan, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaDepartment of Virology, University of Helsinki, Helsinki, FinlandHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaTellurite [Te(IV)] is a high-toxicity metalloid. In this study, a fungus with high Te(IV) resistance was isolated. Strain AB1 could efficiently reduce highly toxic Te(IV) to less toxic Te(0). The reduced products formed rod-shaped biogenetic Te(0) nanoparticles (Bio-TeNPs) intracellularly. Further TEM-element mapping, FTIR, and XPS analysis showed that the extracted Bio-TeNPs ranged from 100 to 500 nm and consisted of Te(0), proteins, lipids, aromatic compounds, and carbohydrates. Moreover, Bio-TeNPs exhibited excellent antibacterial ability against Shigella dysenteriae, Escherichia coli, Enterobacter sakazakii, and Salmonella typhimurium according to inhibition zone tests. Further growth and live/dead staining experiments showed that E. coli and S. typhimurium were significantly inhibited by Bio-TeNPs, and cells were broken or shriveled after treatment with Bio-TeNPs based on SEM observation. Additionally, the antioxidant and cytotoxicity tests showed that the Bio-TeNPs exhibited excellent antioxidant capacity with no cytotoxicity. All these results suggested that strain AB1 showed great potential in bioremediation and Bio-TeNPs were excellent antibacterial nanomaterials with no cytotoxicity.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1020179/fullMortierellaTe(IV) reductionBio-TeNPsantibacterialcytotoxicity |
| spellingShingle | Bo Ao Fei He Jing Lv Junming Tu Zheng Tan Honglin Jiang Xiaoshan Shi Jingjing Li Jingjing Li Jianjun Hou Yuanliang Hu Xian Xia Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity Frontiers in Microbiology Mortierella Te(IV) reduction Bio-TeNPs antibacterial cytotoxicity |
| title | Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity |
| title_full | Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity |
| title_fullStr | Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity |
| title_full_unstemmed | Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity |
| title_short | Green synthesis of biogenetic Te(0) nanoparticles by high tellurite tolerance fungus Mortierella sp. AB1 with antibacterial activity |
| title_sort | green synthesis of biogenetic te 0 nanoparticles by high tellurite tolerance fungus mortierella sp ab1 with antibacterial activity |
| topic | Mortierella Te(IV) reduction Bio-TeNPs antibacterial cytotoxicity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1020179/full |
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