Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation
Dental caries is a diet–biofilm-dependent disease. <i>Streptococcus mutans</i> contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipotei...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/9/2232 |
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| author | Midian C. Castillo Pedraza Erick Dante de Oliveira Fratucelli Sabrina Marcela Ribeiro Elkin Jahir Florez Salamanca Jaqueline da Silva Colin Marlise I. Klein |
| author_facet | Midian C. Castillo Pedraza Erick Dante de Oliveira Fratucelli Sabrina Marcela Ribeiro Elkin Jahir Florez Salamanca Jaqueline da Silva Colin Marlise I. Klein |
| author_sort | Midian C. Castillo Pedraza |
| collection | DOAJ |
| description | Dental caries is a diet–biofilm-dependent disease. <i>Streptococcus mutans</i> contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on <i>S. mutans</i> biofilms. In vitro <i>S. mutans</i> UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 µg/mL), myricetin (500 µg/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of <i>S. mutans</i> biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin. |
| first_indexed | 2024-03-10T19:56:42Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:56:42Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ae320c79ec08439f888cf29acf86af932023-11-19T23:54:27ZengMDPI AGMolecules1420-30492020-05-01259223210.3390/molecules25092232Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm AccumulationMidian C. Castillo Pedraza0Erick Dante de Oliveira Fratucelli1Sabrina Marcela Ribeiro2Elkin Jahir Florez Salamanca3Jaqueline da Silva Colin4Marlise I. Klein5Department of Dental Materials and Prosthodontics, School of Dentistry, Sao Paulo State University (UNESP), Rua Humaita, 1680, Araraquara 14801-903, Sao Paulo, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, Sao Paulo State University (UNESP), Rua Humaita, 1680, Araraquara 14801-903, Sao Paulo, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, Sao Paulo State University (UNESP), Rua Humaita, 1680, Araraquara 14801-903, Sao Paulo, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, Sao Paulo State University (UNESP), Rua Humaita, 1680, Araraquara 14801-903, Sao Paulo, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, Sao Paulo State University (UNESP), Rua Humaita, 1680, Araraquara 14801-903, Sao Paulo, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, Sao Paulo State University (UNESP), Rua Humaita, 1680, Araraquara 14801-903, Sao Paulo, BrazilDental caries is a diet–biofilm-dependent disease. <i>Streptococcus mutans</i> contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on <i>S. mutans</i> biofilms. In vitro <i>S. mutans</i> UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 µg/mL), myricetin (500 µg/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of <i>S. mutans</i> biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin.https://www.mdpi.com/1420-3049/25/9/2232biofilm<i>Streptococcus mutans</i>compound 1771myricetinexopolysaccharideslipoteichoic acids |
| spellingShingle | Midian C. Castillo Pedraza Erick Dante de Oliveira Fratucelli Sabrina Marcela Ribeiro Elkin Jahir Florez Salamanca Jaqueline da Silva Colin Marlise I. Klein Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation Molecules biofilm <i>Streptococcus mutans</i> compound 1771 myricetin exopolysaccharides lipoteichoic acids |
| title | Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation |
| title_full | Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation |
| title_fullStr | Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation |
| title_full_unstemmed | Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation |
| title_short | Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents <i>Streptococcus mutans</i> Biofilm Accumulation |
| title_sort | modulation of lipoteichoic acids and exopolysaccharides prevents i streptococcus mutans i biofilm accumulation |
| topic | biofilm <i>Streptococcus mutans</i> compound 1771 myricetin exopolysaccharides lipoteichoic acids |
| url | https://www.mdpi.com/1420-3049/25/9/2232 |
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