A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity
Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray represen...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2001-10-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996101904231 |
| _version_ | 1819119860948402176 |
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| author | Tanya Barrett Tao Xie Yulan Piao Ora Dillon-Carter George J. Kargul Meng K. Lim Francis J. Chrest Robert Wersto Daniel L. Rowley Magdalena Juhaszova Li Zhou Marquis P. Vawter Kevin G. Becker Christopher Cheadle William H. Wood, III Una D. McCann William J. Freed Minoru S. Ko George A. Ricaurte David M. Donovan |
| author_facet | Tanya Barrett Tao Xie Yulan Piao Ora Dillon-Carter George J. Kargul Meng K. Lim Francis J. Chrest Robert Wersto Daniel L. Rowley Magdalena Juhaszova Li Zhou Marquis P. Vawter Kevin G. Becker Christopher Cheadle William H. Wood, III Una D. McCann William J. Freed Minoru S. Ko George A. Ricaurte David M. Donovan |
| author_sort | Tanya Barrett |
| collection | DOAJ |
| description | Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter–lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity. |
| first_indexed | 2024-12-22T06:11:29Z |
| format | Article |
| id | doaj.art-ae35049b820b4ddbb3e38f9ab69ced28 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-22T06:11:29Z |
| publishDate | 2001-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-ae35049b820b4ddbb3e38f9ab69ced282022-12-21T18:36:12ZengElsevierNeurobiology of Disease1095-953X2001-10-0185822833A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine NeurotoxicityTanya Barrett0Tao Xie1Yulan Piao2Ora Dillon-Carter3George J. Kargul4Meng K. Lim5Francis J. Chrest6Robert Wersto7Daniel L. Rowley8Magdalena Juhaszova9Li Zhou10Marquis P. Vawter11Kevin G. Becker12Christopher Cheadle13William H. Wood, III14Una D. McCann15William J. Freed16Minoru S. Ko17George A. Ricaurte18David M. Donovan19Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive; Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter–lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.http://www.sciencedirect.com/science/article/pii/S0969996101904231dopaminecDNA librarycDNA microarraymethamphetaminedevelopmentgene expression profile |
| spellingShingle | Tanya Barrett Tao Xie Yulan Piao Ora Dillon-Carter George J. Kargul Meng K. Lim Francis J. Chrest Robert Wersto Daniel L. Rowley Magdalena Juhaszova Li Zhou Marquis P. Vawter Kevin G. Becker Christopher Cheadle William H. Wood, III Una D. McCann William J. Freed Minoru S. Ko George A. Ricaurte David M. Donovan A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity Neurobiology of Disease dopamine cDNA library cDNA microarray methamphetamine development gene expression profile |
| title | A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity |
| title_full | A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity |
| title_fullStr | A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity |
| title_full_unstemmed | A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity |
| title_short | A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity |
| title_sort | murine dopamine neuron specific cdna library and microarray increased coxi expression during methamphetamine neurotoxicity |
| topic | dopamine cDNA library cDNA microarray methamphetamine development gene expression profile |
| url | http://www.sciencedirect.com/science/article/pii/S0969996101904231 |
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