From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport
Abstract Background Bud sports occur spontaneously in plants when new growth exhibits a distinct phenotype from the rest of the parent plant. The Witch’s Broom bud sport occurs occasionally in various grapevine (Vitis vinifera) varieties and displays a suite of developmental defects, including dwarf...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-04992-y |
| _version_ | 1797199561972776960 |
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| author | Eleanore J. Ritter Peter Cousins Michelle Quigley Aidan Kile Sunil K. Kenchanmane Raju Daniel H. Chitwood Chad Niederhuth |
| author_facet | Eleanore J. Ritter Peter Cousins Michelle Quigley Aidan Kile Sunil K. Kenchanmane Raju Daniel H. Chitwood Chad Niederhuth |
| author_sort | Eleanore J. Ritter |
| collection | DOAJ |
| description | Abstract Background Bud sports occur spontaneously in plants when new growth exhibits a distinct phenotype from the rest of the parent plant. The Witch’s Broom bud sport occurs occasionally in various grapevine (Vitis vinifera) varieties and displays a suite of developmental defects, including dwarf features and reduced fertility. While it is highly detrimental for grapevine growers, it also serves as a useful tool for studying grapevine development. We used the Witch’s Broom bud sport in grapevine to understand the developmental trajectories of the bud sports, as well as the potential genetic basis. We analyzed the phenotypes of two independent cases of the Witch’s Broom bud sport, in the Dakapo and Merlot varieties of grapevine, alongside wild type counterparts. To do so, we quantified various shoot traits, performed 3D X-ray Computed Tomography on dormant buds, and landmarked leaves from the samples. We also performed Illumina and Oxford Nanopore sequencing on the samples and called genetic variants using these sequencing datasets. Results The Dakapo and Merlot cases of Witch’s Broom displayed severe developmental defects, with no fruit/clusters formed and dwarf vegetative features. However, the Dakapo and Merlot cases of Witch’s Broom studied were also phenotypically different from one another, with distinct differences in bud and leaf development. We identified 968–974 unique genetic mutations in our two Witch’s Broom cases that are potential causal variants of the bud sports. Examining gene function and validating these genetic candidates through PCR and Sanger-sequencing revealed one strong candidate mutation in Merlot Witch’s Broom impacting the gene GSVIVG01008260001. Conclusions The Witch’s Broom bud sports in both varieties studied had dwarf phenotypes, but the two instances studied were also vastly different from one another and likely have distinct genetic bases. Future work on Witch’s Broom bud sports in grapevine could provide more insight into development and the genetic pathways involved in grapevine. |
| first_indexed | 2024-04-24T07:17:43Z |
| format | Article |
| id | doaj.art-ae3694388daa40fda8011981386a0618 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-24T07:17:43Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-ae3694388daa40fda8011981386a06182024-04-21T11:13:47ZengBMCBMC Plant Biology1471-22292024-04-0124111610.1186/s12870-024-04992-yFrom buds to shoots: insights into grapevine development from the Witch’s Broom bud sportEleanore J. Ritter0Peter Cousins1Michelle Quigley2Aidan Kile3Sunil K. Kenchanmane Raju4Daniel H. Chitwood5Chad Niederhuth6Department of Plant Biology, Michigan State UniversityE. & J. Gallo WineryDepartment of Horticulture, Michigan State UniversityDepartment of Plant Biology, Michigan State UniversityDepartment of Plant Biology, Michigan State UniversityDepartment of Horticulture, Michigan State UniversityDepartment of Plant Biology, Michigan State UniversityAbstract Background Bud sports occur spontaneously in plants when new growth exhibits a distinct phenotype from the rest of the parent plant. The Witch’s Broom bud sport occurs occasionally in various grapevine (Vitis vinifera) varieties and displays a suite of developmental defects, including dwarf features and reduced fertility. While it is highly detrimental for grapevine growers, it also serves as a useful tool for studying grapevine development. We used the Witch’s Broom bud sport in grapevine to understand the developmental trajectories of the bud sports, as well as the potential genetic basis. We analyzed the phenotypes of two independent cases of the Witch’s Broom bud sport, in the Dakapo and Merlot varieties of grapevine, alongside wild type counterparts. To do so, we quantified various shoot traits, performed 3D X-ray Computed Tomography on dormant buds, and landmarked leaves from the samples. We also performed Illumina and Oxford Nanopore sequencing on the samples and called genetic variants using these sequencing datasets. Results The Dakapo and Merlot cases of Witch’s Broom displayed severe developmental defects, with no fruit/clusters formed and dwarf vegetative features. However, the Dakapo and Merlot cases of Witch’s Broom studied were also phenotypically different from one another, with distinct differences in bud and leaf development. We identified 968–974 unique genetic mutations in our two Witch’s Broom cases that are potential causal variants of the bud sports. Examining gene function and validating these genetic candidates through PCR and Sanger-sequencing revealed one strong candidate mutation in Merlot Witch’s Broom impacting the gene GSVIVG01008260001. Conclusions The Witch’s Broom bud sports in both varieties studied had dwarf phenotypes, but the two instances studied were also vastly different from one another and likely have distinct genetic bases. Future work on Witch’s Broom bud sports in grapevine could provide more insight into development and the genetic pathways involved in grapevine.https://doi.org/10.1186/s12870-024-04992-yDevelopmentGrapevineVitis viniferaBud sportSomatic mutationsClonal propagation |
| spellingShingle | Eleanore J. Ritter Peter Cousins Michelle Quigley Aidan Kile Sunil K. Kenchanmane Raju Daniel H. Chitwood Chad Niederhuth From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport BMC Plant Biology Development Grapevine Vitis vinifera Bud sport Somatic mutations Clonal propagation |
| title | From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport |
| title_full | From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport |
| title_fullStr | From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport |
| title_full_unstemmed | From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport |
| title_short | From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport |
| title_sort | from buds to shoots insights into grapevine development from the witch s broom bud sport |
| topic | Development Grapevine Vitis vinifera Bud sport Somatic mutations Clonal propagation |
| url | https://doi.org/10.1186/s12870-024-04992-y |
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