Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge
BackgroundInformation on efficacy of a novel bivalent vaccine containing porcine circovirus type 2d (PCV2d) and Mycoplasma hyopneumoniae.ObjectiveTo evaluate bivalent vaccine for efficacy under experimental conditions.AnimalsClinically healthy 35 weaned piglets at 18 days of age were used.MethodsA 2...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1176091/full |
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| author | Sehyeong Ham Jeongmin Suh Taehwan Oh Chonghan Kim Byoung-Joo Seo Chanhee Chae |
| author_facet | Sehyeong Ham Jeongmin Suh Taehwan Oh Chonghan Kim Byoung-Joo Seo Chanhee Chae |
| author_sort | Sehyeong Ham |
| collection | DOAJ |
| description | BackgroundInformation on efficacy of a novel bivalent vaccine containing porcine circovirus type 2d (PCV2d) and Mycoplasma hyopneumoniae.ObjectiveTo evaluate bivalent vaccine for efficacy under experimental conditions.AnimalsClinically healthy 35 weaned piglets at 18 days of age were used.MethodsA 2.0 mL dose of bivalent vaccine was administered intramuscularly to pigs at 21 days of age in accordance with the manufacturer’s instructions. The pigs were challenged at 42 days of age either intranasally with PCV2d, or intratracheally with M. hyopneumoniae, or with both.ResultsVaccinated-challenged pigs improved the growth performance compared to pigs that were unvaccinated and then, challenged. Vaccinated-challenged pigs elicited a significant amount of protective immunity for PCV2d-specific neutralizing antibodies and interferon-γ secreting cells (IFN-γ-SC) as well as for M. hyopneumoniae-specific IFN-γ-SC compared to unvaccinated/challenged pigs. Induction of systemic cellular and humoral immune responses from bivalent vaccination reduced the viral and mycoplasmal loads in the blood and larynx. Vaccination and challenge simultaneously reduced both lung and lymphoid lesion severity when compared to unvaccinated-challenged pigs.DiscussionThe results of this study demonstrated that the evaluated bivalent PCV2d and M. hyopneumoniae vaccine was efficacious in protecting pigs from the most predominant PCV2d genotype in the field today, as evaluated with a dual PCV2d and M. hyopneumoniae challenge under experimental conditions. |
| first_indexed | 2024-03-12T21:48:30Z |
| format | Article |
| id | doaj.art-ae36fb51cd67435da84e93cfacd7b5c5 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-12T21:48:30Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ae36fb51cd67435da84e93cfacd7b5c52023-07-26T07:15:37ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-07-011010.3389/fvets.2023.11760911176091Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challengeSehyeong Ham0Jeongmin Suh1Taehwan Oh2Chonghan Kim3Byoung-Joo Seo4Chanhee Chae5Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaWOOGENE B&G CO., LTD., Seoul, Republic of KoreaWOOGENE B&G CO., LTD., Seoul, Republic of KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaBackgroundInformation on efficacy of a novel bivalent vaccine containing porcine circovirus type 2d (PCV2d) and Mycoplasma hyopneumoniae.ObjectiveTo evaluate bivalent vaccine for efficacy under experimental conditions.AnimalsClinically healthy 35 weaned piglets at 18 days of age were used.MethodsA 2.0 mL dose of bivalent vaccine was administered intramuscularly to pigs at 21 days of age in accordance with the manufacturer’s instructions. The pigs were challenged at 42 days of age either intranasally with PCV2d, or intratracheally with M. hyopneumoniae, or with both.ResultsVaccinated-challenged pigs improved the growth performance compared to pigs that were unvaccinated and then, challenged. Vaccinated-challenged pigs elicited a significant amount of protective immunity for PCV2d-specific neutralizing antibodies and interferon-γ secreting cells (IFN-γ-SC) as well as for M. hyopneumoniae-specific IFN-γ-SC compared to unvaccinated/challenged pigs. Induction of systemic cellular and humoral immune responses from bivalent vaccination reduced the viral and mycoplasmal loads in the blood and larynx. Vaccination and challenge simultaneously reduced both lung and lymphoid lesion severity when compared to unvaccinated-challenged pigs.DiscussionThe results of this study demonstrated that the evaluated bivalent PCV2d and M. hyopneumoniae vaccine was efficacious in protecting pigs from the most predominant PCV2d genotype in the field today, as evaluated with a dual PCV2d and M. hyopneumoniae challenge under experimental conditions.https://www.frontiersin.org/articles/10.3389/fvets.2023.1176091/fullbivalent vaccineenzootic pneumoniaMycoplasma hyopneumoniaeporcine circovirus type 2dporcine respiratory disease complex |
| spellingShingle | Sehyeong Ham Jeongmin Suh Taehwan Oh Chonghan Kim Byoung-Joo Seo Chanhee Chae Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge Frontiers in Veterinary Science bivalent vaccine enzootic pneumonia Mycoplasma hyopneumoniae porcine circovirus type 2d porcine respiratory disease complex |
| title | Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge |
| title_full | Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge |
| title_fullStr | Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge |
| title_full_unstemmed | Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge |
| title_short | Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge |
| title_sort | efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and mycoplasma hyopneumoniae against a dual pcv2d and mycoplasma hyopneumoniae challenge |
| topic | bivalent vaccine enzootic pneumonia Mycoplasma hyopneumoniae porcine circovirus type 2d porcine respiratory disease complex |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1176091/full |
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