Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals
This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve...
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MDPI AG
2020-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/3/281 |
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| author | Mingfei Pan Tianyu Ma Jingying Yang Shijie Li Shengmiao Liu Shuo Wang |
| author_facet | Mingfei Pan Tianyu Ma Jingying Yang Shijie Li Shengmiao Liu Shuo Wang |
| author_sort | Mingfei Pan |
| collection | DOAJ |
| description | This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 μg L<sup>−1</sup> and 60 μg kg<sup>−1</sup>, and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 μg L<sup>−1</sup> and 40 μg kg<sup>−1</sup>, respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B<sub>1</sub>, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B<sub>1</sub>. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples. |
| first_indexed | 2024-04-13T11:35:30Z |
| format | Article |
| id | doaj.art-ae370cbc2f7a42b5bbc05af1ef335a35 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-13T11:35:30Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ae370cbc2f7a42b5bbc05af1ef335a352022-12-22T02:48:27ZengMDPI AGFoods2304-81582020-03-019328110.3390/foods9030281foods9030281Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in CerealsMingfei Pan0Tianyu Ma1Jingying Yang2Shijie Li3Shengmiao Liu4Shuo Wang5State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaThis paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 μg L<sup>−1</sup> and 60 μg kg<sup>−1</sup>, and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 μg L<sup>−1</sup> and 40 μg kg<sup>−1</sup>, respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B<sub>1</sub>, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B<sub>1</sub>. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples.https://www.mdpi.com/2304-8158/9/3/281zearalenoneimmunochromatographic assaycolloidal au sphereau nanorodscereals |
| spellingShingle | Mingfei Pan Tianyu Ma Jingying Yang Shijie Li Shengmiao Liu Shuo Wang Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals Foods zearalenone immunochromatographic assay colloidal au sphere au nanorods cereals |
| title | Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals |
| title_full | Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals |
| title_fullStr | Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals |
| title_full_unstemmed | Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals |
| title_short | Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals |
| title_sort | development of lateral flow immunochromatographic assays using colloidal au sphere and nanorods as signal marker for the determination of zearalenone in cereals |
| topic | zearalenone immunochromatographic assay colloidal au sphere au nanorods cereals |
| url | https://www.mdpi.com/2304-8158/9/3/281 |
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