Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study
Introduction: Oropharyngeal candidiasis is an opportunistic mucosal infection caused by Candida albicans. It usually responds to topical treatments such as clotrimazole troches, topical fluconazole, chlorhexidine mouthwash and nystatin suspension. Piper betel leaf oil and Origanum vulgare essential...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Journal of Indian Association of Public Health Dentistry |
| Subjects: | |
| Online Access: | http://www.jiaphd.org/article.asp?issn=2319-5932;year=2017;volume=15;issue=1;spage=89;epage=93;aulast=Makkar |
| _version_ | 1818195236558471168 |
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| author | Nisha Makkar Sapna B Prasanna Hitashi Singla |
| author_facet | Nisha Makkar Sapna B Prasanna Hitashi Singla |
| author_sort | Nisha Makkar |
| collection | DOAJ |
| description | Introduction: Oropharyngeal candidiasis is an opportunistic mucosal infection caused by Candida albicans. It usually responds to topical treatments such as clotrimazole troches, topical fluconazole, chlorhexidine mouthwash and nystatin suspension. Piper betel leaf oil and Origanum vulgare essential oil have shown some topical antifungal activity. Aim: To determine and compare the antifungal efficacy of piper betel leaf oil, O. vulgare essential oil and fluconazole suspension against C. albicans. Materials and Methods: The zone of inhibition was measured by the cup–plate diffusion method using 100 μl volume of piper betel leaf oil, O. vulgare essential oil and fluconazole suspension, which were pipetted into the wells of the inoculated Sabouraud’s dextrose agar plates. The zone of inhibition was measured in millimetres using Vernier calliper. Minimum inhibitory concentration (MIC) was determined by the broth macro-dilution test by pouring 1 ml of the respective concentrations of the test material to the individual test tubes along with 10 μl of the diluted test organism inoculum. Finally, MIC was calculated using a reflective viewer. Results: The zone of inhibition for O. vulgare essential oil (>40 mm) was more than fluconazole suspension (>35 mm). MIC of O. vulgare essential oil, piper betel leaf oil and fluconazole suspension was 1.6%, 0.4% and 0.8%, respectively. Conclusion: O. vulgare essential oil was found to be a more effective antifungal agent than piper betel leaf oil and fluconazole suspension. |
| first_indexed | 2024-12-12T01:14:59Z |
| format | Article |
| id | doaj.art-ae46becdc4074de694a45e868b367bfa |
| institution | Directory Open Access Journal |
| issn | 2319-5932 2350-0484 |
| language | English |
| last_indexed | 2024-12-12T01:14:59Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Association of Public Health Dentistry |
| spelling | doaj.art-ae46becdc4074de694a45e868b367bfa2022-12-22T00:43:22ZengWolters Kluwer Medknow PublicationsJournal of Indian Association of Public Health Dentistry2319-59322350-04842017-01-01151899310.4103/jiaphd.jiaphd_134_16Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro StudyNisha MakkarSapna B PrasannaHitashi SinglaIntroduction: Oropharyngeal candidiasis is an opportunistic mucosal infection caused by Candida albicans. It usually responds to topical treatments such as clotrimazole troches, topical fluconazole, chlorhexidine mouthwash and nystatin suspension. Piper betel leaf oil and Origanum vulgare essential oil have shown some topical antifungal activity. Aim: To determine and compare the antifungal efficacy of piper betel leaf oil, O. vulgare essential oil and fluconazole suspension against C. albicans. Materials and Methods: The zone of inhibition was measured by the cup–plate diffusion method using 100 μl volume of piper betel leaf oil, O. vulgare essential oil and fluconazole suspension, which were pipetted into the wells of the inoculated Sabouraud’s dextrose agar plates. The zone of inhibition was measured in millimetres using Vernier calliper. Minimum inhibitory concentration (MIC) was determined by the broth macro-dilution test by pouring 1 ml of the respective concentrations of the test material to the individual test tubes along with 10 μl of the diluted test organism inoculum. Finally, MIC was calculated using a reflective viewer. Results: The zone of inhibition for O. vulgare essential oil (>40 mm) was more than fluconazole suspension (>35 mm). MIC of O. vulgare essential oil, piper betel leaf oil and fluconazole suspension was 1.6%, 0.4% and 0.8%, respectively. Conclusion: O. vulgare essential oil was found to be a more effective antifungal agent than piper betel leaf oil and fluconazole suspension.http://www.jiaphd.org/article.asp?issn=2319-5932;year=2017;volume=15;issue=1;spage=89;epage=93;aulast=MakkarCandida albicansfluconazolein vitro studyoriganum vulgare essential oilpiper betel leaf oil |
| spellingShingle | Nisha Makkar Sapna B Prasanna Hitashi Singla Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study Journal of Indian Association of Public Health Dentistry Candida albicans fluconazole in vitro study origanum vulgare essential oil piper betel leaf oil |
| title | Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study |
| title_full | Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study |
| title_fullStr | Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study |
| title_full_unstemmed | Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study |
| title_short | Comparative Evaluation of Antifungal Activity of Piper Betel Leaf Oil, Origanum vulgare Essential Oil and Fluconazole Suspension on Candida albicans − An In Vitro Study |
| title_sort | comparative evaluation of antifungal activity of piper betel leaf oil origanum vulgare essential oil and fluconazole suspension on candida albicans an in vitro study |
| topic | Candida albicans fluconazole in vitro study origanum vulgare essential oil piper betel leaf oil |
| url | http://www.jiaphd.org/article.asp?issn=2319-5932;year=2017;volume=15;issue=1;spage=89;epage=93;aulast=Makkar |
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