An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins
The outbreak of the coronavirus disease 2019 (COVID-19) in December 2019 has highlighted the need for a flexible sensing system that can quickly and accurately determine the presence of biomarkers associated with the disease. This sensing system also needs to be easily adaptable to incorporate both...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/5/347 |
| _version_ | 1827669994911039488 |
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| author | Thomas Farrow Siriny Laumier Ian Sandall Harm van Zalinge |
| author_facet | Thomas Farrow Siriny Laumier Ian Sandall Harm van Zalinge |
| author_sort | Thomas Farrow |
| collection | DOAJ |
| description | The outbreak of the coronavirus disease 2019 (COVID-19) in December 2019 has highlighted the need for a flexible sensing system that can quickly and accurately determine the presence of biomarkers associated with the disease. This sensing system also needs to be easily adaptable to incorporate both novel diseases as well as changes in the existing ones. Here we report the feasibility of using a simple, low-cost silicon field-effect transistor functionalised with aptamers and designed to attach to the spike protein of SARS-CoV2. It is shown that a linear response can be obtained in a concentration range of 100 fM to 10 pM. Furthermore, by using a larger range of source-drain potentials compared with other FET based sensors, it is possible to look at a wider range of device parameters to optimise the response. |
| first_indexed | 2024-03-10T03:14:51Z |
| format | Article |
| id | doaj.art-ae46fba965ba4e37a3cf751754542d46 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T03:14:51Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-ae46fba965ba4e37a3cf751754542d462023-11-23T10:16:12ZengMDPI AGBiosensors2079-63742022-05-0112534710.3390/bios12050347An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of ProteinsThomas Farrow0Siriny Laumier1Ian Sandall2Harm van Zalinge3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool L69 3GJ, UKDepartment of Electrical Engineering and Electronics, University of Liverpool, Liverpool L69 3GJ, UKDepartment of Electrical Engineering and Electronics, University of Liverpool, Liverpool L69 3GJ, UKDepartment of Electrical Engineering and Electronics, University of Liverpool, Liverpool L69 3GJ, UKThe outbreak of the coronavirus disease 2019 (COVID-19) in December 2019 has highlighted the need for a flexible sensing system that can quickly and accurately determine the presence of biomarkers associated with the disease. This sensing system also needs to be easily adaptable to incorporate both novel diseases as well as changes in the existing ones. Here we report the feasibility of using a simple, low-cost silicon field-effect transistor functionalised with aptamers and designed to attach to the spike protein of SARS-CoV2. It is shown that a linear response can be obtained in a concentration range of 100 fM to 10 pM. Furthermore, by using a larger range of source-drain potentials compared with other FET based sensors, it is possible to look at a wider range of device parameters to optimise the response.https://www.mdpi.com/2079-6374/12/5/347biosensorsthin film sensorsfield effect transistorsaptamers |
| spellingShingle | Thomas Farrow Siriny Laumier Ian Sandall Harm van Zalinge An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins Biosensors biosensors thin film sensors field effect transistors aptamers |
| title | An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins |
| title_full | An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins |
| title_fullStr | An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins |
| title_full_unstemmed | An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins |
| title_short | An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins |
| title_sort | aptamer functionalised schottky field effect transistor for the detection of proteins |
| topic | biosensors thin film sensors field effect transistors aptamers |
| url | https://www.mdpi.com/2079-6374/12/5/347 |
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