Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease
Abstract Objective To identify differences in the expression of peroxisome proliferator-activated receptor alpha (PPARα) target genes in human peripheral blood mononuclear cells (PBMCs) associated with non-alcoholic fatty liver disease (NAFLD) among Chinese individuals. Methods Thirty healthy subjec...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-11-01
|
| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-018-0901-7 |
| _version_ | 1818084781265518592 |
|---|---|
| author | Tian Tian Li Tian Bi Tan Hai Qing Hou Xiao Yun Zhao |
| author_facet | Tian Tian Li Tian Bi Tan Hai Qing Hou Xiao Yun Zhao |
| author_sort | Tian Tian Li |
| collection | DOAJ |
| description | Abstract Objective To identify differences in the expression of peroxisome proliferator-activated receptor alpha (PPARα) target genes in human peripheral blood mononuclear cells (PBMCs) associated with non-alcoholic fatty liver disease (NAFLD) among Chinese individuals. Methods Thirty healthy subjects were selected as the control group (CN), and 43 patients newly diagnosed with NAFLD were subdivided into two groups, non-obese group (NF, n = 21) and obese group (OF, n = 22). Expression of PPARα and its target genes was determined in PBMCs. The levels of liver cell damage markers, total cholesterol (TC), triglyceride (TG), free fatty acids (FFA), glucose, and insulin were determined in serum. Results Compared to the CN group, the blood pressure and homeostasis model assessment for insulin resistance (HOMA-IR) were increased in the other groups (P < 0.05), while the systolic blood pressure (SBP) and liver cell damage markers were significantly increased in the OF group (P < 0.05). In the OF group, PPARα target gene expression was 2.03–3.31 times higher than that in the CN group, and a negative correlation was found between PPARα target gene expression and abdominal circumference (AC), body mass index (BMI), diastolic blood pressure (DBP). Additionally, solute carrier family 25 (carnitine/acylcarnitine translocase) member 20 (SLC25A20) and acyl-coenzyme A dehydrogenase 2 long chain (ACADVL) were negatively correlated with HOMA-IR; PPARα, acetyl-coenzyme A dehydrogenase 2 (ACAA2), and carnitine palmitoyltransferase 1A (CPT1A) were positively correlated with HOMA-IR. Conclusion There is an up-expression of PPARα target genes in the PBMCs of NAFLD patients, possibly leading to changes in β-oxidation and insulin resistance. |
| first_indexed | 2024-12-10T19:59:20Z |
| format | Article |
| id | doaj.art-ae4743f3d7864df78c7010c20e907af5 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-10T19:59:20Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-ae4743f3d7864df78c7010c20e907af52022-12-22T01:35:35ZengBMCLipids in Health and Disease1476-511X2018-11-011711810.1186/s12944-018-0901-7Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver diseaseTian Tian Li0Tian Bi Tan1Hai Qing Hou2Xiao Yun Zhao3Medical Experimental Center of Qilu Hospital (Qingdao), Shandong UniversityDynacareClinical Lab of Qilu Hospital (Qingdao), Shandong UniversityClinical Lab of Qilu Hospital (Qingdao), Shandong UniversityAbstract Objective To identify differences in the expression of peroxisome proliferator-activated receptor alpha (PPARα) target genes in human peripheral blood mononuclear cells (PBMCs) associated with non-alcoholic fatty liver disease (NAFLD) among Chinese individuals. Methods Thirty healthy subjects were selected as the control group (CN), and 43 patients newly diagnosed with NAFLD were subdivided into two groups, non-obese group (NF, n = 21) and obese group (OF, n = 22). Expression of PPARα and its target genes was determined in PBMCs. The levels of liver cell damage markers, total cholesterol (TC), triglyceride (TG), free fatty acids (FFA), glucose, and insulin were determined in serum. Results Compared to the CN group, the blood pressure and homeostasis model assessment for insulin resistance (HOMA-IR) were increased in the other groups (P < 0.05), while the systolic blood pressure (SBP) and liver cell damage markers were significantly increased in the OF group (P < 0.05). In the OF group, PPARα target gene expression was 2.03–3.31 times higher than that in the CN group, and a negative correlation was found between PPARα target gene expression and abdominal circumference (AC), body mass index (BMI), diastolic blood pressure (DBP). Additionally, solute carrier family 25 (carnitine/acylcarnitine translocase) member 20 (SLC25A20) and acyl-coenzyme A dehydrogenase 2 long chain (ACADVL) were negatively correlated with HOMA-IR; PPARα, acetyl-coenzyme A dehydrogenase 2 (ACAA2), and carnitine palmitoyltransferase 1A (CPT1A) were positively correlated with HOMA-IR. Conclusion There is an up-expression of PPARα target genes in the PBMCs of NAFLD patients, possibly leading to changes in β-oxidation and insulin resistance.http://link.springer.com/article/10.1186/s12944-018-0901-7NAFLDObesityInsulin resistancePBMCPPARα |
| spellingShingle | Tian Tian Li Tian Bi Tan Hai Qing Hou Xiao Yun Zhao Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease Lipids in Health and Disease NAFLD Obesity Insulin resistance PBMC PPARα |
| title | Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease |
| title_full | Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease |
| title_fullStr | Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease |
| title_full_unstemmed | Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease |
| title_short | Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease |
| title_sort | changes in peroxisome proliferator activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non alcoholic fatty liver disease |
| topic | NAFLD Obesity Insulin resistance PBMC PPARα |
| url | http://link.springer.com/article/10.1186/s12944-018-0901-7 |
| work_keys_str_mv | AT tiantianli changesinperoxisomeproliferatoractivatedreceptoralphatargetgeneexpressioninperipheralbloodmononuclearcellsassociatedwithnonalcoholicfattyliverdisease AT tianbitan changesinperoxisomeproliferatoractivatedreceptoralphatargetgeneexpressioninperipheralbloodmononuclearcellsassociatedwithnonalcoholicfattyliverdisease AT haiqinghou changesinperoxisomeproliferatoractivatedreceptoralphatargetgeneexpressioninperipheralbloodmononuclearcellsassociatedwithnonalcoholicfattyliverdisease AT xiaoyunzhao changesinperoxisomeproliferatoractivatedreceptoralphatargetgeneexpressioninperipheralbloodmononuclearcellsassociatedwithnonalcoholicfattyliverdisease |