Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients
The data presented in this article are related to the research article entitled âBehcet Disease serum is immunoreactive to neurofilament medium which share common epitopes to bacterial HSP-65, a putative triggerâ (Lule et a. 2017) [1]. The immunoreactivity to self-antigens is well characterized for...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340917304584 |
| _version_ | 1818512891428470784 |
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| author | Ayse Ilksen Colpak Banu Balci-Peynircioglu Alp Can Yasemin Gursoy-Ozdemir Sevda Lule Umut Kalyoncu Turgay Dalkara |
| author_facet | Ayse Ilksen Colpak Banu Balci-Peynircioglu Alp Can Yasemin Gursoy-Ozdemir Sevda Lule Umut Kalyoncu Turgay Dalkara |
| author_sort | Ayse Ilksen Colpak |
| collection | DOAJ |
| description | The data presented in this article are related to the research article entitled âBehcet Disease serum is immunoreactive to neurofilament medium which share common epitopes to bacterial HSP-65, a putative triggerâ (Lule et a. 2017) [1]. The immunoreactivity to self-antigens is well characterized for systemic lupus erythematosus (SLE) and multiple sclerosis (MS) (Magro Checa et al., 2013) [2]. Indirect immunofluorescence labeling of the mouse tissue sections with patient sera has recently been popular to discover novel epitopes and gain mechanistic insight to diseases with dysregulated immunity (Lennon et al., 2004) [3]. The present article demonstrates widespread labeling of cell nuclei with SLE patient sera and sporadic filamentous labeling along the axons with MS patient sera on mouse brain sections. The filamentous immunolabeling was sometimes associated with cytoplasmic staining of cells, which sent processes along the axon bundles, suggesting that they were oligodendrocytes. Since the mouse brain tissue has little autofluorescence and limited connective tissue causing non-specific immunolabeling, it appears superior to peripheral tissues for searching serum immunoreactivity. Keywords: Indirect immunofluorescence, Multiple sclerosis, Systemic lupus erythematosus, Autoimmunity |
| first_indexed | 2024-12-10T23:53:20Z |
| format | Article |
| id | doaj.art-ae47d3ed25504879a64923e83ed8b55b |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-10T23:53:20Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-ae47d3ed25504879a64923e83ed8b55b2022-12-22T01:28:41ZengElsevierData in Brief2352-34092017-12-0115170173Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patientsAyse Ilksen Colpak0Banu Balci-Peynircioglu1Alp Can2Yasemin Gursoy-Ozdemir3Sevda Lule4Umut Kalyoncu5Turgay Dalkara6Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, TurkeyInstitute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Current affiliation: Department of Medical Biology, Faculty of Medicine, Hacettepe University, Ankara, TurkeyDepartment of Histology and Embryology, School of Medicine, Ankara University, Ankara, TurkeyInstitute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Current affiliation: Department of Neurology, School of Medicine (KUSOM), Koç University, Ä°stanbul, TurkeyInstitute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, TurkeyDepartment of Internal Medicine, Division of Rheumatology, Faculty of Medicine, Hacettepe University, Ankara, TurkeyDepartment of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Institute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Corresponding author at: Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.The data presented in this article are related to the research article entitled âBehcet Disease serum is immunoreactive to neurofilament medium which share common epitopes to bacterial HSP-65, a putative triggerâ (Lule et a. 2017) [1]. The immunoreactivity to self-antigens is well characterized for systemic lupus erythematosus (SLE) and multiple sclerosis (MS) (Magro Checa et al., 2013) [2]. Indirect immunofluorescence labeling of the mouse tissue sections with patient sera has recently been popular to discover novel epitopes and gain mechanistic insight to diseases with dysregulated immunity (Lennon et al., 2004) [3]. The present article demonstrates widespread labeling of cell nuclei with SLE patient sera and sporadic filamentous labeling along the axons with MS patient sera on mouse brain sections. The filamentous immunolabeling was sometimes associated with cytoplasmic staining of cells, which sent processes along the axon bundles, suggesting that they were oligodendrocytes. Since the mouse brain tissue has little autofluorescence and limited connective tissue causing non-specific immunolabeling, it appears superior to peripheral tissues for searching serum immunoreactivity. Keywords: Indirect immunofluorescence, Multiple sclerosis, Systemic lupus erythematosus, Autoimmunityhttp://www.sciencedirect.com/science/article/pii/S2352340917304584 |
| spellingShingle | Ayse Ilksen Colpak Banu Balci-Peynircioglu Alp Can Yasemin Gursoy-Ozdemir Sevda Lule Umut Kalyoncu Turgay Dalkara Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients Data in Brief |
| title | Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients |
| title_full | Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients |
| title_fullStr | Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients |
| title_full_unstemmed | Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients |
| title_short | Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients |
| title_sort | data of indirect immunofluorescence labeling of the mouse brain sections with sera from sle and ms patients |
| url | http://www.sciencedirect.com/science/article/pii/S2352340917304584 |
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