Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene
In <i>Arabidopsis thaliana</i>, the breaking of seed dormancy in wild type (Col-0) by ethylene at 100 μL L<sup>−1</sup> required at least 30 h application. A mutant of the proteolytic N-degron pathway, lacking the E3 ligase <i>PROTEOLYSIS 6</i> (<i>PRT6</...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9352 |
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| author | Xu Wang Marlène Davanture Michel Zivy Christophe Bailly Eiji Nambara Françoise Corbineau |
| author_facet | Xu Wang Marlène Davanture Michel Zivy Christophe Bailly Eiji Nambara Françoise Corbineau |
| author_sort | Xu Wang |
| collection | DOAJ |
| description | In <i>Arabidopsis thaliana</i>, the breaking of seed dormancy in wild type (Col-0) by ethylene at 100 μL L<sup>−1</sup> required at least 30 h application. A mutant of the proteolytic N-degron pathway, lacking the E3 ligase <i>PROTEOLYSIS 6</i> (<i>PRT6</i>), was investigated for its role in ethylene-triggered changes in proteomes during seed germination. Label-free quantitative proteomics was carried out on dormant wild type Col-0 and <i>prt6</i> seeds treated with (+) or without (−) ethylene. After 16 h, 1737 proteins were identified, but none was significantly different in protein levels in response to ethylene. After longer ethylene treatment (30 h), 2552 proteins were identified, and 619 Differentially Expressed Proteins (DEPs) had significant differences in protein abundances between ethylene treatments and genotypes. In Col, 587 DEPs were enriched for those involved in signal perception and transduction, reserve mobilization and new material generation, which potentially contributed to seed germination. DEPs up-regulated by ethylene in Col included S-adenosylmethionine synthase 1, methionine adenosyltransferase 3 and ACC oxidase involved in ethylene synthesis and of Pyrabactin Resistance1 acting as an ABA receptor, while DEPs down-regulated by ethylene in Col included aldehyde oxidase 4 involved in ABA synthesis. In contrast, in <i>prt6</i> seeds, ethylene did not result in strong proteomic changes with only 30 DEPs. Taken together, the present work demonstrates that the proteolytic N-degron pathway is essential for ethylene-mediated reprogramming of seed proteomes during germination. |
| first_indexed | 2024-03-09T09:55:38Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:55:38Z |
| publishDate | 2022-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ae487eea821544b3a391a902dba2ad512023-12-01T23:48:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316935210.3390/ijms23169352Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to EthyleneXu Wang0Marlène Davanture1Michel Zivy2Christophe Bailly3Eiji Nambara4Françoise Corbineau5Biologie des Semences, UMR 7622, IBPS, Sorbonne Université, 4 Place Jussieu, 75005 Paris, FranceGQE-Le Moulon, PAPPSO, INRAE, CNRS, AgroParisTech, Université Paris-Saclay, 91190 Gif-sur-Yvette, FranceGQE-Le Moulon, PAPPSO, INRAE, CNRS, AgroParisTech, Université Paris-Saclay, 91190 Gif-sur-Yvette, FranceBiologie des Semences, UMR 7622, IBPS, Sorbonne Université, 4 Place Jussieu, 75005 Paris, FranceDepartment of Cell & Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 3B2, CanadaBiologie des Semences, UMR 7622, IBPS, Sorbonne Université, 4 Place Jussieu, 75005 Paris, FranceIn <i>Arabidopsis thaliana</i>, the breaking of seed dormancy in wild type (Col-0) by ethylene at 100 μL L<sup>−1</sup> required at least 30 h application. A mutant of the proteolytic N-degron pathway, lacking the E3 ligase <i>PROTEOLYSIS 6</i> (<i>PRT6</i>), was investigated for its role in ethylene-triggered changes in proteomes during seed germination. Label-free quantitative proteomics was carried out on dormant wild type Col-0 and <i>prt6</i> seeds treated with (+) or without (−) ethylene. After 16 h, 1737 proteins were identified, but none was significantly different in protein levels in response to ethylene. After longer ethylene treatment (30 h), 2552 proteins were identified, and 619 Differentially Expressed Proteins (DEPs) had significant differences in protein abundances between ethylene treatments and genotypes. In Col, 587 DEPs were enriched for those involved in signal perception and transduction, reserve mobilization and new material generation, which potentially contributed to seed germination. DEPs up-regulated by ethylene in Col included S-adenosylmethionine synthase 1, methionine adenosyltransferase 3 and ACC oxidase involved in ethylene synthesis and of Pyrabactin Resistance1 acting as an ABA receptor, while DEPs down-regulated by ethylene in Col included aldehyde oxidase 4 involved in ABA synthesis. In contrast, in <i>prt6</i> seeds, ethylene did not result in strong proteomic changes with only 30 DEPs. Taken together, the present work demonstrates that the proteolytic N-degron pathway is essential for ethylene-mediated reprogramming of seed proteomes during germination.https://www.mdpi.com/1422-0067/23/16/9352<i>Arabidopsis thaliana</i>seed dormancyN-end rule pathwayethylenequantitative proteomicslabel-free |
| spellingShingle | Xu Wang Marlène Davanture Michel Zivy Christophe Bailly Eiji Nambara Françoise Corbineau Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> seed dormancy N-end rule pathway ethylene quantitative proteomics label-free |
| title | Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene |
| title_full | Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene |
| title_fullStr | Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene |
| title_full_unstemmed | Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene |
| title_short | Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in <i>Arabidopsis thaliana</i> Seed Responsiveness to Ethylene |
| title_sort | label free quantitative proteomics reveal the involvement of prt6 in i arabidopsis thaliana i seed responsiveness to ethylene |
| topic | <i>Arabidopsis thaliana</i> seed dormancy N-end rule pathway ethylene quantitative proteomics label-free |
| url | https://www.mdpi.com/1422-0067/23/16/9352 |
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