Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation
Objectives: Cyclin-dependent kinases (CDKs) including Cdk9 have been associated with cardiac differentiation. The increasing evidence has proposed that Cdk9 overexpression can regulate the epigenome. However, the current research is the first report of the Cdk9 affection on the regional and global D...
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2022-01-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
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| Online Access: | http://www.cjmb.org/pdf.php?id=514 |
| _version_ | 1819277280797523968 |
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| author | Leila Abkhooie Mostafa Moradi Sarabi Houman Kahroba Hossein Ghanbarian Soheila Montazer Saheb Vahideh Tarhriz Mohammad Saeid Hejazi |
| author_facet | Leila Abkhooie Mostafa Moradi Sarabi Houman Kahroba Hossein Ghanbarian Soheila Montazer Saheb Vahideh Tarhriz Mohammad Saeid Hejazi |
| author_sort | Leila Abkhooie |
| collection | DOAJ |
| description | Objectives: Cyclin-dependent kinases (CDKs) including Cdk9 have been associated with cardiac differentiation. The increasing evidence has proposed that Cdk9 overexpression can regulate the epigenome. However, the current research is the first report of the Cdk9 affection on the regional and global DNA methylation during differentiation. Materials and Methods: This study examined the effects of Cdk9 overexpression on the regional methylation patterns of cardiac miRNAs (miR-1, -133, -206) and myogenic regulatory factors (i.e., MyoD and Myogenin) and promoter DNA methylation in mouse myoblast C2C12 cells during differentiation by the methylation-specific polymerase chain reaction (MSP-PCR) method. Moreover, the mRNA expression levels of DNMT1, DNMT3A, DNMT3B, and global 5-methyl cytosine (5-mC) levels in mouse myoblast C2C12 cells were quantified during differentiation by RT-qPCR and ELISA methods, respectively. Results: The results demonstrated that Cdk9 overexpression results in DNA methylation changes in mouse myoblast C2C12 cells. It was found that the average expression levels of DNMTs in line with global DNA methylation significantly increased in Cdk9 transfected cells upon Cdk9 overexpression (P<0.05). In addition, the results showed that the regional promoter methylation of miR-1 and miR-133 genes increased in transfected cells during differentiation. An interesting possibility raised by our study is that further active global DNA methylation observed in Cdk9-transfected C2C12 cells can be clarified through the increased DNMT expression by Cdk9 in these cells. Conclusions: In general, our study provides a comprehensive mechanism that Cdk9 can promote epigenetic changes and modulate global and regional DNA methylation profiling of myoblast cells during differentiation. |
| first_indexed | 2024-12-23T23:53:37Z |
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| institution | Directory Open Access Journal |
| issn | 2148-9696 |
| language | English |
| last_indexed | 2024-12-23T23:53:37Z |
| publishDate | 2022-01-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-ae4b58d160e94ca2aea420bf4cfa04ba2022-12-21T17:25:19ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962022-01-0191243210.34172/cjmb.2022.05cjmb-8Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During DifferentiationLeila Abkhooie0Mostafa Moradi Sarabi1Houman Kahroba2Hossein Ghanbarian3Soheila Montazer Saheb4Vahideh Tarhriz5Mohammad Saeid Hejazi6Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Clinical Laboratory Sciences, School of Allied Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.Objectives: Cyclin-dependent kinases (CDKs) including Cdk9 have been associated with cardiac differentiation. The increasing evidence has proposed that Cdk9 overexpression can regulate the epigenome. However, the current research is the first report of the Cdk9 affection on the regional and global DNA methylation during differentiation. Materials and Methods: This study examined the effects of Cdk9 overexpression on the regional methylation patterns of cardiac miRNAs (miR-1, -133, -206) and myogenic regulatory factors (i.e., MyoD and Myogenin) and promoter DNA methylation in mouse myoblast C2C12 cells during differentiation by the methylation-specific polymerase chain reaction (MSP-PCR) method. Moreover, the mRNA expression levels of DNMT1, DNMT3A, DNMT3B, and global 5-methyl cytosine (5-mC) levels in mouse myoblast C2C12 cells were quantified during differentiation by RT-qPCR and ELISA methods, respectively. Results: The results demonstrated that Cdk9 overexpression results in DNA methylation changes in mouse myoblast C2C12 cells. It was found that the average expression levels of DNMTs in line with global DNA methylation significantly increased in Cdk9 transfected cells upon Cdk9 overexpression (P<0.05). In addition, the results showed that the regional promoter methylation of miR-1 and miR-133 genes increased in transfected cells during differentiation. An interesting possibility raised by our study is that further active global DNA methylation observed in Cdk9-transfected C2C12 cells can be clarified through the increased DNMT expression by Cdk9 in these cells. Conclusions: In general, our study provides a comprehensive mechanism that Cdk9 can promote epigenetic changes and modulate global and regional DNA methylation profiling of myoblast cells during differentiation.http://www.cjmb.org/pdf.php?id=514micrornacdk9dna methylationgene expressionmyoblast cell differentiation |
| spellingShingle | Leila Abkhooie Mostafa Moradi Sarabi Houman Kahroba Hossein Ghanbarian Soheila Montazer Saheb Vahideh Tarhriz Mohammad Saeid Hejazi Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation Crescent Journal of Medical and Biological Sciences microrna cdk9 dna methylation gene expression myoblast cell differentiation |
| title | Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation |
| title_full | Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation |
| title_fullStr | Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation |
| title_full_unstemmed | Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation |
| title_short | Cyclin-dependent Kinase 9 Induces Regional and Global Genomic DNA Methylation Via Influencing DNMT Gene Expression in Mouse Myoblast C2C12 Cells During Differentiation |
| title_sort | cyclin dependent kinase 9 induces regional and global genomic dna methylation via influencing dnmt gene expression in mouse myoblast c2c12 cells during differentiation |
| topic | microrna cdk9 dna methylation gene expression myoblast cell differentiation |
| url | http://www.cjmb.org/pdf.php?id=514 |
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