A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses
Rice blast caused by Magnaporthe oryzae is one of the most important diseases of rice. Elicitors secreted by M. oryzae play important roles in the interaction with rice to facilitate fungal infection and disease development. In recent years, several elicitor proteins have been identified in M. oryza...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1018616/full |
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| author | Yanfang Nie Yanfang Nie Guanjun Li Jieling Li Xiaoshu Zhou Yanzhi Zhang Qingchuan Shi Xiaofan Zhou Huaping Li Xiao-Lin Chen Yunfeng Li |
| author_facet | Yanfang Nie Yanfang Nie Guanjun Li Jieling Li Xiaoshu Zhou Yanzhi Zhang Qingchuan Shi Xiaofan Zhou Huaping Li Xiao-Lin Chen Yunfeng Li |
| author_sort | Yanfang Nie |
| collection | DOAJ |
| description | Rice blast caused by Magnaporthe oryzae is one of the most important diseases of rice. Elicitors secreted by M. oryzae play important roles in the interaction with rice to facilitate fungal infection and disease development. In recent years, several elicitor proteins have been identified in M. oryzae, and their functions and importance are increasingly appreciated. In this study, we purified a novel elicitor-activity protein from M. oryzae, which was further identified as a vanadium chloroperoxidase (MoVcpo) by MAIDL TOF/TOF MS. The purified MoVcpo induced reactive oxygen species (ROS) accumulation in host cells, up-regulated the expression of multiple defense-related genes, thus significantly enhancing rice resistance against M. oryzae. These results suggested that MoVcpo functions as a pathogen-associated molecular pattern (PAMP) to trigger rice immunity. Furthermore, MoVcpo was highly expressed in the early stage of M. oryzae infection. Deletion of MoVcpo affected spore formation, conidia germination, cell wall integrity, and sensitivity to osmotic stress, but not fungal growth. Interestingly, compared with the wild-type, inoculation with MoVcpo deletion mutant on rice led to markedly induced ROS accumulation, increased expression of defense-related genes, but also lower disease severity, suggesting that MoVcpo acts as both an elicitor activating plant immune responses and a virulence factor facilitating fungal infection. These findings reveal a novel role for vanadium chloroperoxidase in fungal pathogenesis and deepen our understanding of M. oryzae-rice interactions. |
| first_indexed | 2024-04-11T09:34:45Z |
| format | Article |
| id | doaj.art-ae4c543947634c20b05bac2b46ea1c0d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T09:34:45Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ae4c543947634c20b05bac2b46ea1c0d2022-12-22T04:31:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10186161018616A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responsesYanfang Nie0Yanfang Nie1Guanjun Li2Jieling Li3Xiaoshu Zhou4Yanzhi Zhang5Qingchuan Shi6Xiaofan Zhou7Huaping Li8Xiao-Lin Chen9Yunfeng Li10College of Materials and Energy, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaState Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, ChinaRice blast caused by Magnaporthe oryzae is one of the most important diseases of rice. Elicitors secreted by M. oryzae play important roles in the interaction with rice to facilitate fungal infection and disease development. In recent years, several elicitor proteins have been identified in M. oryzae, and their functions and importance are increasingly appreciated. In this study, we purified a novel elicitor-activity protein from M. oryzae, which was further identified as a vanadium chloroperoxidase (MoVcpo) by MAIDL TOF/TOF MS. The purified MoVcpo induced reactive oxygen species (ROS) accumulation in host cells, up-regulated the expression of multiple defense-related genes, thus significantly enhancing rice resistance against M. oryzae. These results suggested that MoVcpo functions as a pathogen-associated molecular pattern (PAMP) to trigger rice immunity. Furthermore, MoVcpo was highly expressed in the early stage of M. oryzae infection. Deletion of MoVcpo affected spore formation, conidia germination, cell wall integrity, and sensitivity to osmotic stress, but not fungal growth. Interestingly, compared with the wild-type, inoculation with MoVcpo deletion mutant on rice led to markedly induced ROS accumulation, increased expression of defense-related genes, but also lower disease severity, suggesting that MoVcpo acts as both an elicitor activating plant immune responses and a virulence factor facilitating fungal infection. These findings reveal a novel role for vanadium chloroperoxidase in fungal pathogenesis and deepen our understanding of M. oryzae-rice interactions.https://www.frontiersin.org/articles/10.3389/fpls.2022.1018616/fullMagnaporthe oryzaeelicitorvanadium chloroperoxidasericereactive oxygen species |
| spellingShingle | Yanfang Nie Yanfang Nie Guanjun Li Jieling Li Xiaoshu Zhou Yanzhi Zhang Qingchuan Shi Xiaofan Zhou Huaping Li Xiao-Lin Chen Yunfeng Li A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses Frontiers in Plant Science Magnaporthe oryzae elicitor vanadium chloroperoxidase rice reactive oxygen species |
| title | A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses |
| title_full | A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses |
| title_fullStr | A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses |
| title_full_unstemmed | A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses |
| title_short | A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses |
| title_sort | novel elicitor movcpo is necessary for the virulence of magnaporthe oryzae and triggers rice defense responses |
| topic | Magnaporthe oryzae elicitor vanadium chloroperoxidase rice reactive oxygen species |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1018616/full |
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