Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue
The sexual maturation occurred by the changes of steroid hormones was known to sex-dependently and/or agedependently regulate the lipid metabolism in various animal species. Our current study demonstrates that lipid and its functional fatty acids can be changed depending on the status of sexual matu...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2015-06-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=1468&vmd=Full |
| _version_ | 1818561108687978496 |
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| author | Chang Seok Park Sang-Rae Cho Young Sik Park |
| author_facet | Chang Seok Park Sang-Rae Cho Young Sik Park |
| author_sort | Chang Seok Park |
| collection | DOAJ |
| description | The sexual maturation occurred by the changes of steroid hormones was known to sex-dependently and/or agedependently regulate the lipid metabolism in various animal species. Our current study demonstrates that lipid and its functional fatty acids can be changed depending on the status of sexual maturation. Of the functional fatty acids, γ- linolenic acid (GLA; 18:3n-6) is an important factor for maintaining human health. The purpose of our study was to investigate the level of GLA in mice with different stages of sexual maturation. To this end, the longissimus muscle (LM) of immature (3-week-old) and mature (7-week-old) female mice was analysed for the fatty acid composition by gas chromatography. Furthermore, both gene and protein level of Δ6 desaturase (FADS2) which is involved in GLA metabolism by real time PCR and Western blotting, respectively. Mature females showed greater (p<0.05) serum 17β -estradiol (E2) level and LM GLA contents than immature group. The mRNA and protein levels of FADS2, which converts precursor linoleic acid into GLA, were higher (p<0.05) in mature female mice than in immature mice. In conclusion, these results show that sexual maturation of female mice induces GLA and FADS2 contents in LM. |
| first_indexed | 2024-12-14T00:46:44Z |
| format | Article |
| id | doaj.art-ae4f0230b017432eb8fe0be8be796a75 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-14T00:46:44Z |
| publishDate | 2015-06-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-ae4f0230b017432eb8fe0be8be796a752022-12-21T23:24:05ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632015-06-01302838910.12750/JET.2015.30.2.83Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle TissueChang Seok Park0Sang-Rae Cho1Young Sik Park2Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 232-950, KoreaHanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 232-950, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 742-711, KoreaThe sexual maturation occurred by the changes of steroid hormones was known to sex-dependently and/or agedependently regulate the lipid metabolism in various animal species. Our current study demonstrates that lipid and its functional fatty acids can be changed depending on the status of sexual maturation. Of the functional fatty acids, γ- linolenic acid (GLA; 18:3n-6) is an important factor for maintaining human health. The purpose of our study was to investigate the level of GLA in mice with different stages of sexual maturation. To this end, the longissimus muscle (LM) of immature (3-week-old) and mature (7-week-old) female mice was analysed for the fatty acid composition by gas chromatography. Furthermore, both gene and protein level of Δ6 desaturase (FADS2) which is involved in GLA metabolism by real time PCR and Western blotting, respectively. Mature females showed greater (p<0.05) serum 17β -estradiol (E2) level and LM GLA contents than immature group. The mRNA and protein levels of FADS2, which converts precursor linoleic acid into GLA, were higher (p<0.05) in mature female mice than in immature mice. In conclusion, these results show that sexual maturation of female mice induces GLA and FADS2 contents in LM.http://www.e-jarb.org/journal/view.html?uid=1468&vmd=Fullsexual maturationsteroid hormonefads2gla |
| spellingShingle | Chang Seok Park Sang-Rae Cho Young Sik Park Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue Journal of Animal Reproduction and Biotechnology sexual maturation steroid hormone fads2 gla |
| title | Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue |
| title_full | Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue |
| title_fullStr | Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue |
| title_full_unstemmed | Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue |
| title_short | Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue |
| title_sort | sexually matured female mice showed higher γ linolenic acid gla in the muscle tissue |
| topic | sexual maturation steroid hormone fads2 gla |
| url | http://www.e-jarb.org/journal/view.html?uid=1468&vmd=Full |
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