The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet
Sesamin is a major lignan in sesame seed. We confirmed that ingestion of sesamin and α-tocopherol synergistically reduced the concentration of blood cholesterol in rats given a high-cholesterol diet. To elucidate the molecular mechanism behind this effect, we analyzed the gene-expression profiles in...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319307704 |
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| author | Tomohiro Rogi Namino Tomimori Yoshiko Ono Yoshinobu Kiso |
| author_facet | Tomohiro Rogi Namino Tomimori Yoshiko Ono Yoshinobu Kiso |
| author_sort | Tomohiro Rogi |
| collection | DOAJ |
| description | Sesamin is a major lignan in sesame seed. We confirmed that ingestion of sesamin and α-tocopherol synergistically reduced the concentration of blood cholesterol in rats given a high-cholesterol diet. To elucidate the molecular mechanism behind this effect, we analyzed the gene-expression profiles in rat liver after co-ingestion of sesamin and α-tocopherol. Six-week-old male Sprague-Dawley rats were fed a 1% cholesterol diet (HC) or HC containing 0.2% sesamin, 1% α-tocopherol or sesamin + α-tocopherol for 10 days. Blood samples were collected on days 1, 3, 7, and 10 and livers were excised on day 10. The gene expressions of ATP-binding cassette, sub-family G (WHITE), members 5 (ABCG5) and 8 (ABCG8) were significantly increased, while the gene expression of apolipoprotein (Apo) A4 was significantly decreased. ABCG5 and ABCG8 form a functional heterodimer that acts as a cholesterol efflux transporter, which contributes to the excretion of cholesterol from the liver. ApoA4 controls the secretion of ApoB, which is a component of low-density-lipoprotein cholesterol. These studies indicate that the cholesterol-lowering mechanism underlying the effects of co-ingestion of sesamin and α-tocopherol might be attributable to increased biliary excretion of cholesterol and reduced ApoB secretion into the blood-stream. Keywords:: sesamin, hypocholesterolemic activity, α-tocopherol, ATP-binding cassette transporter, apolipoprotein |
| first_indexed | 2024-04-12T19:11:29Z |
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| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-12T19:11:29Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier |
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| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-ae4f240198ad4daea26a33ee4427685b2022-12-22T03:19:51ZengElsevierJournal of Pharmacological Sciences1347-86132011-01-011153408416The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol DietTomohiro Rogi0Namino Tomimori1Yoshiko Ono2Yoshinobu Kiso3Institute for Health Care Science, Suntory Wellness Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan; Corresponding author. Tomohiro_Rogi@suntory.co.jpInstitute for Health Care Science, Suntory Wellness Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, JapanInstitute for Health Care Science, Suntory Wellness Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, JapanInstitute for Health Care Science, Suntory Wellness Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, JapanSesamin is a major lignan in sesame seed. We confirmed that ingestion of sesamin and α-tocopherol synergistically reduced the concentration of blood cholesterol in rats given a high-cholesterol diet. To elucidate the molecular mechanism behind this effect, we analyzed the gene-expression profiles in rat liver after co-ingestion of sesamin and α-tocopherol. Six-week-old male Sprague-Dawley rats were fed a 1% cholesterol diet (HC) or HC containing 0.2% sesamin, 1% α-tocopherol or sesamin + α-tocopherol for 10 days. Blood samples were collected on days 1, 3, 7, and 10 and livers were excised on day 10. The gene expressions of ATP-binding cassette, sub-family G (WHITE), members 5 (ABCG5) and 8 (ABCG8) were significantly increased, while the gene expression of apolipoprotein (Apo) A4 was significantly decreased. ABCG5 and ABCG8 form a functional heterodimer that acts as a cholesterol efflux transporter, which contributes to the excretion of cholesterol from the liver. ApoA4 controls the secretion of ApoB, which is a component of low-density-lipoprotein cholesterol. These studies indicate that the cholesterol-lowering mechanism underlying the effects of co-ingestion of sesamin and α-tocopherol might be attributable to increased biliary excretion of cholesterol and reduced ApoB secretion into the blood-stream. Keywords:: sesamin, hypocholesterolemic activity, α-tocopherol, ATP-binding cassette transporter, apolipoproteinhttp://www.sciencedirect.com/science/article/pii/S1347861319307704 |
| spellingShingle | Tomohiro Rogi Namino Tomimori Yoshiko Ono Yoshinobu Kiso The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet Journal of Pharmacological Sciences |
| title | The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet |
| title_full | The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet |
| title_fullStr | The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet |
| title_full_unstemmed | The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet |
| title_short | The Mechanism Underlying the Synergetic Hypocholesterolemic Effect of Sesamin and α-Tocopherol in Rats Fed a High-Cholesterol Diet |
| title_sort | mechanism underlying the synergetic hypocholesterolemic effect of sesamin and α tocopherol in rats fed a high cholesterol diet |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319307704 |
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