Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture
This study examined the potential benefits of male specific-pathogen-free (SPF) White Leghorn embryos in cellular agriculture for sustainable and ethical poultry meat production—addressing traditional farming challenges, including disease outbreaks of <i>Salmonella</i> and Avian influenz...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/11/1887 |
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| author | Won-Seok Ju Kangmin Seo Bo-Ram Lee Mi-Ryung Park Min-Gook Lee Sung-June Byun Hyeon Yang Youngim Kim Sun-A Ock |
| author_facet | Won-Seok Ju Kangmin Seo Bo-Ram Lee Mi-Ryung Park Min-Gook Lee Sung-June Byun Hyeon Yang Youngim Kim Sun-A Ock |
| author_sort | Won-Seok Ju |
| collection | DOAJ |
| description | This study examined the potential benefits of male specific-pathogen-free (SPF) White Leghorn embryos in cellular agriculture for sustainable and ethical poultry meat production—addressing traditional farming challenges, including disease outbreaks of <i>Salmonella</i> and Avian influenza. We isolated myogenic precursor cells (MPCs) from the thigh muscles (<i>Musculus femoris</i>) of 12.5-day-old embryos from 10 SPF White Leghorns that tested negative for <i>Salmonella</i>. We randomly selected MPCs from three males and three females, isolated them using a modified pre-plating (pp) method, and compared their in vitro development. After 1 h (pp1) and 2 h (pp2) of incubation, they were transferred to a new dish to remove fast-adhering cells and cultured (pp3). Isolated MPCs had a 69% positive reaction to Pax7. During proliferation, no differences were observed in <i>PAX7, MYF5</i>, or <i>MYOD</i> expression between the male and female MPCs. However, after five days of differentiation, the expression of late myogenic factors—<i>MYOG</i> and <i>MYF6—</i>significantly increased in all MPCs. Notably, <i>MYOG</i> expression was 1.9 times higher in female than in male MPCs. This impacted <i>MYMK</i>’s expression pattern. Despite this, the myotube fusion index did not differ between the sexes. Muscle cells from male SPF-laying chicken embryos are promising for developing clean animal-cell-derived protein sources via resource recycling. |
| first_indexed | 2024-03-11T03:12:45Z |
| format | Article |
| id | doaj.art-ae4f4665223443e78b9d65e042036c76 |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T03:12:45Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ae4f4665223443e78b9d65e042036c762023-11-18T07:31:01ZengMDPI AGAnimals2076-26152023-06-011311188710.3390/ani13111887Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro AgricultureWon-Seok Ju0Kangmin Seo1Bo-Ram Lee2Mi-Ryung Park3Min-Gook Lee4Sung-June Byun5Hyeon Yang6Youngim Kim7Sun-A Ock8Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaAnimal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500 Kongjwipatjwi-ro, Wanju-gun 565-851, JB, Republic of KoreaThis study examined the potential benefits of male specific-pathogen-free (SPF) White Leghorn embryos in cellular agriculture for sustainable and ethical poultry meat production—addressing traditional farming challenges, including disease outbreaks of <i>Salmonella</i> and Avian influenza. We isolated myogenic precursor cells (MPCs) from the thigh muscles (<i>Musculus femoris</i>) of 12.5-day-old embryos from 10 SPF White Leghorns that tested negative for <i>Salmonella</i>. We randomly selected MPCs from three males and three females, isolated them using a modified pre-plating (pp) method, and compared their in vitro development. After 1 h (pp1) and 2 h (pp2) of incubation, they were transferred to a new dish to remove fast-adhering cells and cultured (pp3). Isolated MPCs had a 69% positive reaction to Pax7. During proliferation, no differences were observed in <i>PAX7, MYF5</i>, or <i>MYOD</i> expression between the male and female MPCs. However, after five days of differentiation, the expression of late myogenic factors—<i>MYOG</i> and <i>MYF6—</i>significantly increased in all MPCs. Notably, <i>MYOG</i> expression was 1.9 times higher in female than in male MPCs. This impacted <i>MYMK</i>’s expression pattern. Despite this, the myotube fusion index did not differ between the sexes. Muscle cells from male SPF-laying chicken embryos are promising for developing clean animal-cell-derived protein sources via resource recycling.https://www.mdpi.com/2076-2615/13/11/1887specific-pathogen-free (SPF) chicken embryocultured meatmyogenic precursor cellssexpoultry<i>Salmonella</i> |
| spellingShingle | Won-Seok Ju Kangmin Seo Bo-Ram Lee Mi-Ryung Park Min-Gook Lee Sung-June Byun Hyeon Yang Youngim Kim Sun-A Ock Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture Animals specific-pathogen-free (SPF) chicken embryo cultured meat myogenic precursor cells sex poultry <i>Salmonella</i> |
| title | Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture |
| title_full | Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture |
| title_fullStr | Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture |
| title_full_unstemmed | Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture |
| title_short | Potential Application of Muscle Precursor Cells from Male Specific-Pathogen-Free (SPF) Chicken Embryos in In Vitro Agriculture |
| title_sort | potential application of muscle precursor cells from male specific pathogen free spf chicken embryos in in vitro agriculture |
| topic | specific-pathogen-free (SPF) chicken embryo cultured meat myogenic precursor cells sex poultry <i>Salmonella</i> |
| url | https://www.mdpi.com/2076-2615/13/11/1887 |
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