Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
Background: Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results: In this study the influence of high temperature on the cellular and m...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-05-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345815000445 |
| _version_ | 1819090658000896000 |
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| author | Min Zhang Jun Shi Li Jiang |
| author_facet | Min Zhang Jun Shi Li Jiang |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | Background: Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production.
Results: In this study the influence of high temperature on the cellular and mitochondrial membrane integrity of Saccharomyces cerevisiae as well as reactive oxygen species (ROS) formation was investigated to understand the mechanisms of the high temperature fermentation process. However, increasing the temperature to 42°C resulted in a clear decrease in the cytoplasmic and mitochondrial membrane potential and an increase in intracellular ROS formation. It was also determined that the different thermostability between YZ1 and YF31 strains had a clear correlation with the yeast's intracellular trehalose content of the cell. Finally, random amplified polymorphic DNA (RAPD) was used to explore the genome differences between the YZ1 and YF31 strains.
Conclusions: Thus, the stability of the mitochondrial membrane and subsequently, the clearance ROS ability could be important factors for the viability of S. cerevisiae at high temperatures. |
| first_indexed | 2024-12-21T22:27:19Z |
| format | Article |
| id | doaj.art-ae50d64d0f8c4771812e7f80b6de81db |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-21T22:27:19Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-ae50d64d0f8c4771812e7f80b6de81db2022-12-21T18:48:10ZengElsevierElectronic Journal of Biotechnology0717-34582015-05-0118320220910.1016/j.ejbt.2015.03.008Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiaeMin Zhang0Jun Shi1Li Jiang2Division of Cardiology, TongRen Hospital, Shanghai JiaoTong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, ChinaKey Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, ChinaDivision of Cardiology, TongRen Hospital, Shanghai JiaoTong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, ChinaBackground: Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results: In this study the influence of high temperature on the cellular and mitochondrial membrane integrity of Saccharomyces cerevisiae as well as reactive oxygen species (ROS) formation was investigated to understand the mechanisms of the high temperature fermentation process. However, increasing the temperature to 42°C resulted in a clear decrease in the cytoplasmic and mitochondrial membrane potential and an increase in intracellular ROS formation. It was also determined that the different thermostability between YZ1 and YF31 strains had a clear correlation with the yeast's intracellular trehalose content of the cell. Finally, random amplified polymorphic DNA (RAPD) was used to explore the genome differences between the YZ1 and YF31 strains. Conclusions: Thus, the stability of the mitochondrial membrane and subsequently, the clearance ROS ability could be important factors for the viability of S. cerevisiae at high temperatures.http://www.sciencedirect.com/science/article/pii/S0717345815000445BioethanolRandom amplified polymorphic DNA (RAPD)Yeast |
| spellingShingle | Min Zhang Jun Shi Li Jiang Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae Electronic Journal of Biotechnology Bioethanol Random amplified polymorphic DNA (RAPD) Yeast |
| title | Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae |
| title_full | Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae |
| title_fullStr | Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae |
| title_full_unstemmed | Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae |
| title_short | Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae |
| title_sort | modulation of mitochondrial membrane integrity and ros formation by high temperature in saccharomyces cerevisiae |
| topic | Bioethanol Random amplified polymorphic DNA (RAPD) Yeast |
| url | http://www.sciencedirect.com/science/article/pii/S0717345815000445 |
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