Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i>
Protein kinases and phosphatases catalyze the phosphorylation and dephosphorylation of their protein substrates, respectively, and these are important mechanisms in cellular signal transduction. The rice blast fungus <i>Magnaporthe oryzae</i> possesses 6 protein phosphatases of type 2C c...
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/1 |
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| author | Jules Biregeya Wilfred M. Anjago Shu Pan Ruina Zhang Zifeng Yang Meilian Chen Abah Felix Huxiao Xu Yaqi Lin Oswald Nkurikiyimfura Yakubu Saddeeq Abubakar Zonghua Wang Wei Tang |
| author_facet | Jules Biregeya Wilfred M. Anjago Shu Pan Ruina Zhang Zifeng Yang Meilian Chen Abah Felix Huxiao Xu Yaqi Lin Oswald Nkurikiyimfura Yakubu Saddeeq Abubakar Zonghua Wang Wei Tang |
| author_sort | Jules Biregeya |
| collection | DOAJ |
| description | Protein kinases and phosphatases catalyze the phosphorylation and dephosphorylation of their protein substrates, respectively, and these are important mechanisms in cellular signal transduction. The rice blast fungus <i>Magnaporthe oryzae</i> possesses 6 protein phosphatases of type 2C class, including MoPtc1, 2, 5, 6, 7 and 8. However, only very little is known about the roles of these phosphatases in filamentous fungi. Here in, we deployed genetics and molecular biology techniques to identify, characterize and establish the roles of MoPtc5 and MoPtc7 in <i>M. oryzae</i> development and pathogenicity. We found that during pathogen-host interaction, <i>MoPTC7</i> is differentially expressed. Double deletion of <i>MoPTC7</i> and <i>MoPTC5</i> suppressed the fungal vegetative growth, altered its cell wall integrity and reduced its virulence. The two genes were found indispensable for stress tolerance in the phytopathogen. We also demonstrated that disruption of any of the two genes highly affected appressorium turgor generation and Mps1 and Osm1 phosphorylation levels. Lastly, we demonstrated that both MoPtc5 and MoPtc7 are localized to mitochondria of different cellular compartments in the blast fungus. Taken together, our study revealed synergistic coordination of <i>M. oryzae</i> development and pathogenesis by the type 2C protein phosphatases. |
| first_indexed | 2024-04-10T21:13:25Z |
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| id | doaj.art-ae50fab5957c41ab973b1e4999e0b134 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-04-10T21:13:25Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-ae50fab5957c41ab973b1e4999e0b1342023-01-20T15:07:54ZengMDPI AGJournal of Fungi2309-608X2022-12-0191110.3390/jof9010001Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i>Jules Biregeya0Wilfred M. Anjago1Shu Pan2Ruina Zhang3Zifeng Yang4Meilian Chen5Abah Felix6Huxiao Xu7Yaqi Lin8Oswald Nkurikiyimfura9Yakubu Saddeeq Abubakar10Zonghua Wang11Wei Tang12State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFuzhou Institute of Oceanography, Minjiang University, Fuzhou 350108, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaProtein kinases and phosphatases catalyze the phosphorylation and dephosphorylation of their protein substrates, respectively, and these are important mechanisms in cellular signal transduction. The rice blast fungus <i>Magnaporthe oryzae</i> possesses 6 protein phosphatases of type 2C class, including MoPtc1, 2, 5, 6, 7 and 8. However, only very little is known about the roles of these phosphatases in filamentous fungi. Here in, we deployed genetics and molecular biology techniques to identify, characterize and establish the roles of MoPtc5 and MoPtc7 in <i>M. oryzae</i> development and pathogenicity. We found that during pathogen-host interaction, <i>MoPTC7</i> is differentially expressed. Double deletion of <i>MoPTC7</i> and <i>MoPTC5</i> suppressed the fungal vegetative growth, altered its cell wall integrity and reduced its virulence. The two genes were found indispensable for stress tolerance in the phytopathogen. We also demonstrated that disruption of any of the two genes highly affected appressorium turgor generation and Mps1 and Osm1 phosphorylation levels. Lastly, we demonstrated that both MoPtc5 and MoPtc7 are localized to mitochondria of different cellular compartments in the blast fungus. Taken together, our study revealed synergistic coordination of <i>M. oryzae</i> development and pathogenesis by the type 2C protein phosphatases.https://www.mdpi.com/2309-608X/9/1/1blast fungusprotein phosphatasesphosphorylationpathogenicity |
| spellingShingle | Jules Biregeya Wilfred M. Anjago Shu Pan Ruina Zhang Zifeng Yang Meilian Chen Abah Felix Huxiao Xu Yaqi Lin Oswald Nkurikiyimfura Yakubu Saddeeq Abubakar Zonghua Wang Wei Tang Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i> Journal of Fungi blast fungus protein phosphatases phosphorylation pathogenicity |
| title | Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i> |
| title_full | Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i> |
| title_fullStr | Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i> |
| title_full_unstemmed | Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i> |
| title_short | Type 2C Protein Phosphatases <i>MoPtc5</i> and <i>MoPtc7</i> Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of <i>Magnaporthe oryzae</i> |
| title_sort | type 2c protein phosphatases i moptc5 i and i moptc7 i are crucial for multiple stress tolerance conidiogenesis and pathogenesis of i magnaporthe oryzae i |
| topic | blast fungus protein phosphatases phosphorylation pathogenicity |
| url | https://www.mdpi.com/2309-608X/9/1/1 |
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