Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis
The lymphatic system is crucial for the regeneration of many tissues due to its fundamental role in immune cell trafficking, protein transport, and tissue homeostasis maintenance. Strategies stimulating lymphangiogenesis can provide new therapeutic approaches for tissue repair and regeneration (e.g....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/2/225 |
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| author | Hanyu Xie Sha Sha Lingbo Lu Geng Wu Hongbing Jiang Aldo R. Boccaccini Kai Zheng Rongyao Xu |
| author_facet | Hanyu Xie Sha Sha Lingbo Lu Geng Wu Hongbing Jiang Aldo R. Boccaccini Kai Zheng Rongyao Xu |
| author_sort | Hanyu Xie |
| collection | DOAJ |
| description | The lymphatic system is crucial for the regeneration of many tissues due to its fundamental role in immune cell trafficking, protein transport, and tissue homeostasis maintenance. Strategies stimulating lymphangiogenesis can provide new therapeutic approaches for tissue repair and regeneration (e.g., chronic wound healing). Here, we explored the effects of cerium-containing mesoporous bioactive glass nanoparticles (Ce-MBGNs) on lymphangiogenesis. The results showed that the extracts of Ce-MBGNs (1, 5, or 10 wt/v%) were non-cytotoxic toward lymphatic endothelial cells (LECs), while they enhanced the proliferation of LECs. Moreover, as evidenced by the scratch wound healing and Transwell migration assays, conditioned media containing the extract of Ce-MBGNs (1 wt/v%) could enhance the migration of LECs in comparison to the blank control and the media containing vascular endothelial growth factor-C (VEGF-C, 50 ng/mL). Additionally, a tube-formation assay using LECs showed that the extract of Ce-MBGNs (1 wt/v%) promoted lymphatic vascular network formation. Western blot results suggested that Ce-MBGNs could induce lymphangiogenesis probably through the HIF-1α/VEGFR-3 pathway. Our study for the first time showed the effects of Ce-MBGNs on stimulating lymphangiogenesis in vitro, highlighting the potential of Ce-MBGNs for wound healing. |
| first_indexed | 2024-03-09T21:15:12Z |
| format | Article |
| id | doaj.art-ae519505859d4a448814018e68d41d39 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:15:12Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-ae519505859d4a448814018e68d41d392023-11-23T21:35:44ZengMDPI AGPharmaceutics1999-49232022-01-0114222510.3390/pharmaceutics14020225Cerium-Containing Bioactive Glasses Promote In Vitro LymphangiogenesisHanyu Xie0Sha Sha1Lingbo Lu2Geng Wu3Hongbing Jiang4Aldo R. Boccaccini5Kai Zheng6Rongyao Xu7Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, ChinaDepartment of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, ChinaInstitute of Biomaterials, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, GermanyJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, ChinaDepartment of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, ChinaThe lymphatic system is crucial for the regeneration of many tissues due to its fundamental role in immune cell trafficking, protein transport, and tissue homeostasis maintenance. Strategies stimulating lymphangiogenesis can provide new therapeutic approaches for tissue repair and regeneration (e.g., chronic wound healing). Here, we explored the effects of cerium-containing mesoporous bioactive glass nanoparticles (Ce-MBGNs) on lymphangiogenesis. The results showed that the extracts of Ce-MBGNs (1, 5, or 10 wt/v%) were non-cytotoxic toward lymphatic endothelial cells (LECs), while they enhanced the proliferation of LECs. Moreover, as evidenced by the scratch wound healing and Transwell migration assays, conditioned media containing the extract of Ce-MBGNs (1 wt/v%) could enhance the migration of LECs in comparison to the blank control and the media containing vascular endothelial growth factor-C (VEGF-C, 50 ng/mL). Additionally, a tube-formation assay using LECs showed that the extract of Ce-MBGNs (1 wt/v%) promoted lymphatic vascular network formation. Western blot results suggested that Ce-MBGNs could induce lymphangiogenesis probably through the HIF-1α/VEGFR-3 pathway. Our study for the first time showed the effects of Ce-MBGNs on stimulating lymphangiogenesis in vitro, highlighting the potential of Ce-MBGNs for wound healing.https://www.mdpi.com/1999-4923/14/2/225bioactive glassesnanoparticlesceriumlymphangiogenesiswound healing |
| spellingShingle | Hanyu Xie Sha Sha Lingbo Lu Geng Wu Hongbing Jiang Aldo R. Boccaccini Kai Zheng Rongyao Xu Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis Pharmaceutics bioactive glasses nanoparticles cerium lymphangiogenesis wound healing |
| title | Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis |
| title_full | Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis |
| title_fullStr | Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis |
| title_full_unstemmed | Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis |
| title_short | Cerium-Containing Bioactive Glasses Promote In Vitro Lymphangiogenesis |
| title_sort | cerium containing bioactive glasses promote in vitro lymphangiogenesis |
| topic | bioactive glasses nanoparticles cerium lymphangiogenesis wound healing |
| url | https://www.mdpi.com/1999-4923/14/2/225 |
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