Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China
Bacteria in root nodules of legumes play important roles in promoting plant growth. In this study, we investigated root nodule-associated bacteria isolated from leguminous plants along an elevation gradient on the northern slope of the Kunlun Mountains, China, using a cultivation approach. In total,...
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Frontiers Media S.A.
2021-02-01
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author | Jinfeng Pang Jinfeng Pang Jinfeng Pang Jinfeng Pang Jinfeng Pang Marike Palmer Henry J. Sun Cale O. Seymour Ling Zhang Ling Zhang Ling Zhang Ling Zhang Brian P. Hedlund Brian P. Hedlund Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng |
author_facet | Jinfeng Pang Jinfeng Pang Jinfeng Pang Jinfeng Pang Jinfeng Pang Marike Palmer Henry J. Sun Cale O. Seymour Ling Zhang Ling Zhang Ling Zhang Ling Zhang Brian P. Hedlund Brian P. Hedlund Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng |
author_sort | Jinfeng Pang |
collection | DOAJ |
description | Bacteria in root nodules of legumes play important roles in promoting plant growth. In this study, we investigated root nodule-associated bacteria isolated from leguminous plants along an elevation gradient on the northern slope of the Kunlun Mountains, China, using a cultivation approach. In total, 300 isolates were obtained from seven legume species within six ecological zones. Isolates were identified based on 16S rRNA gene phylogenetic analysis and potential rhizobia were further identified using a recA gene phylogeny. Among the isolates, Bacillales (particularly Bacillus) were the dominant isolates from all host legumes and all elevations (63.5%), followed by Rhizobiales (13%) and Pseudomonadales (11.7%). Less than 3% of the isolates belonged to Burkholderiales, Paenibacillales, Enterobacteriales, Actinomycetales, Sphingomonadales, Xanthomonadales, Chitinophagales, Brevibacillales, Staphylococcales, or Mycobacteriales. A few elevation-specific patterns emerged within the Bacillales and Pseudomonadales. For example, isolates related to the psychrotroph Bacillus psychrosaccharolyticus were only isolated from the highest elevation sites (>3,500 m) whereas those related to the mesophile Bacillus endophyticus were only isolated from lowest elevation sites (1,350 m), suggestive of a role of soil temperature in their distribution. Similarly, isolates related to Pseudomonas brassicacearum were the dominant Pseudomonadales isolates, but they were only isolated from middle and low elevations (<3,200 m). A total of 39 isolates belonged to the Rhizobiales, 36 of which were confirmed to the genus level using the recA gene. In all, Rhizobiales isolates were obtained from five different host legumes spanning the entire elevation gradient. Those from the low-elevation Qira Desert-Oasis Transition Zone (1,350–1,960 m) suggested some patterns of host preference. For example, most isolates from Albizia julibrissin formed a monophyletic group related to Rhizobium lemnae and most from Alhagi sparsifolia were closely related to Ensifer kummerowiae. In general, this study shows that most bacteria associated with root nodules of legumes are widely distributed in distinct ecological zones within a single geographic region but suggests that both climate and host interactions may influence their distributions. |
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spelling | doaj.art-ae5240afd24a4a96aff877e4b2aa38142022-12-21T23:00:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-02-011210.3389/fmicb.2021.633141633141Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, ChinaJinfeng Pang0Jinfeng Pang1Jinfeng Pang2Jinfeng Pang3Jinfeng Pang4Marike Palmer5Henry J. Sun6Cale O. Seymour7Ling Zhang8Ling Zhang9Ling Zhang10Ling Zhang11Brian P. Hedlund12Brian P. Hedlund13Fanjiang Zeng14Fanjiang Zeng15Fanjiang Zeng16Fanjiang Zeng17State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, ChinaXinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, ChinaCele National Station of Observation and Research for Desert Grassland Ecosystem in Xinjiang, Cele, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaSchool of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, United StatesSchool of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, United StatesDesert Research Institute, Las Vegas, NV, United StatesSchool of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, United StatesState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, ChinaXinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, ChinaCele National Station of Observation and Research for Desert Grassland Ecosystem in Xinjiang, Cele, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaSchool of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, United StatesNevada Institute of Personalized Medicine, Las Vegas, NV, United StatesState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, ChinaXinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, ChinaCele National Station of Observation and Research for Desert Grassland Ecosystem in Xinjiang, Cele, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaBacteria in root nodules of legumes play important roles in promoting plant growth. In this study, we investigated root nodule-associated bacteria isolated from leguminous plants along an elevation gradient on the northern slope of the Kunlun Mountains, China, using a cultivation approach. In total, 300 isolates were obtained from seven legume species within six ecological zones. Isolates were identified based on 16S rRNA gene phylogenetic analysis and potential rhizobia were further identified using a recA gene phylogeny. Among the isolates, Bacillales (particularly Bacillus) were the dominant isolates from all host legumes and all elevations (63.5%), followed by Rhizobiales (13%) and Pseudomonadales (11.7%). Less than 3% of the isolates belonged to Burkholderiales, Paenibacillales, Enterobacteriales, Actinomycetales, Sphingomonadales, Xanthomonadales, Chitinophagales, Brevibacillales, Staphylococcales, or Mycobacteriales. A few elevation-specific patterns emerged within the Bacillales and Pseudomonadales. For example, isolates related to the psychrotroph Bacillus psychrosaccharolyticus were only isolated from the highest elevation sites (>3,500 m) whereas those related to the mesophile Bacillus endophyticus were only isolated from lowest elevation sites (1,350 m), suggestive of a role of soil temperature in their distribution. Similarly, isolates related to Pseudomonas brassicacearum were the dominant Pseudomonadales isolates, but they were only isolated from middle and low elevations (<3,200 m). A total of 39 isolates belonged to the Rhizobiales, 36 of which were confirmed to the genus level using the recA gene. In all, Rhizobiales isolates were obtained from five different host legumes spanning the entire elevation gradient. Those from the low-elevation Qira Desert-Oasis Transition Zone (1,350–1,960 m) suggested some patterns of host preference. For example, most isolates from Albizia julibrissin formed a monophyletic group related to Rhizobium lemnae and most from Alhagi sparsifolia were closely related to Ensifer kummerowiae. In general, this study shows that most bacteria associated with root nodules of legumes are widely distributed in distinct ecological zones within a single geographic region but suggests that both climate and host interactions may influence their distributions.https://www.frontiersin.org/articles/10.3389/fmicb.2021.633141/fullmicrobial diversityendophytesroot noduleslegumes (Fabaceae)elevationKunlun Mountains |
spellingShingle | Jinfeng Pang Jinfeng Pang Jinfeng Pang Jinfeng Pang Jinfeng Pang Marike Palmer Henry J. Sun Cale O. Seymour Ling Zhang Ling Zhang Ling Zhang Ling Zhang Brian P. Hedlund Brian P. Hedlund Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Fanjiang Zeng Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China Frontiers in Microbiology microbial diversity endophytes root nodules legumes (Fabaceae) elevation Kunlun Mountains |
title | Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China |
title_full | Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China |
title_fullStr | Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China |
title_full_unstemmed | Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China |
title_short | Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China |
title_sort | diversity of root nodule associated bacteria of diverse legumes along an elevation gradient in the kunlun mountains china |
topic | microbial diversity endophytes root nodules legumes (Fabaceae) elevation Kunlun Mountains |
url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.633141/full |
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