miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes
Yongquan Chen, Yecheng Deng, Linghua Chen, Ziyao Huang, Yi Yan, Zhaoqi Huang Department of Cardiology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaCorrespondence: Zhaoqi Huang, Department of Cardiology, The Third Affiliated Hospital of Guangzho...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-03-01
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| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/mir-16-5p-regulates-ferroptosis-by-targeting-slc7a11-in-adriamycin-ind-peer-reviewed-fulltext-article-JIR |
| _version_ | 1797870956378587136 |
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| author | Chen Y Deng Y Chen L Huang Z Yan Y Huang Z |
| author_facet | Chen Y Deng Y Chen L Huang Z Yan Y Huang Z |
| author_sort | Chen Y |
| collection | DOAJ |
| description | Yongquan Chen, Yecheng Deng, Linghua Chen, Ziyao Huang, Yi Yan, Zhaoqi Huang Department of Cardiology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaCorrespondence: Zhaoqi Huang, Department of Cardiology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510150, People’s Republic of China, Email zhaoqihuang2007@163.comIntroduction: Adriamycin (ADR) is commonly used in tumor chemotherapy, but its nonreversible cardiotoxicity severely hampers its clinical application. Ferroptosis is an implicated cause of ADR-induced injury. However, the underlying molecular mechanisms remain poorly understood. This study explored whether ferroptosis is a pivotal pathogenic pathway underlying ADR-induced cardiotoxicity and the possible molecular mechanisms involved.Methods: In vivo and in vitro experimental models were used to study the mechanism of ADR-mediated ferroptosis. Ferroptosis levels were examined in mice and human/rat cardiomyocytes. Mechanistically, the expression levels of SLC7A11 and related miRNAs were examined. Bioinformatics prediction and luciferase reporter assays were used to verify the potential interaction between miR-16-5p and SLC7A11. The biological functions and interaction of SLC7A11 and miR-16-5p were investigated in vivo and in vitro.Results: Our study observed that ADR treatment significantly increased ferroptosis levels in vivo and in vitro. Ferroptosis-related pharmacological interventions further confirmed these results. Our data displayed that the SLC7A11 level was significantly decreased in cardiac tissues and cells, while an increased expression level of miR-16-5p was observed. Moreover, upregulation of SLC7A1 and inhibition of miR-16-5p attenuated ADR-induced cardiomyocyte ferroptosis injury. Interactive rescue experiments showed that the protective effects of miR-16-5p inhibition on ADR-induced cardiomyocyte injury were blocked by SLC7A11 knockdown.Discussion: Based on these findings, targeting miR-16-5p could partially reverse the ADR-induced cardiotoxicity by rescuing the SLC7A11 to attenuate ferroptosis. This study presents a pre-clinical basis to identify miR-16-5p/SLC7A11 as a potential treatment target for ADR-induced cardiotoxicity.Keywords: adriamycin, cardiomyocytes, ferroptosis, SLC7A11, miR-16-5p |
| first_indexed | 2024-04-10T00:35:32Z |
| format | Article |
| id | doaj.art-ae52f658c08847cdbddd21dd3b0c6156 |
| institution | Directory Open Access Journal |
| issn | 1178-7031 |
| language | English |
| last_indexed | 2024-04-10T00:35:32Z |
| publishDate | 2023-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj.art-ae52f658c08847cdbddd21dd3b0c61562023-03-14T18:21:58ZengDove Medical PressJournal of Inflammation Research1178-70312023-03-01Volume 161077108982248miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in CardiomyocytesChen YDeng YChen LHuang ZYan YHuang ZYongquan Chen, Yecheng Deng, Linghua Chen, Ziyao Huang, Yi Yan, Zhaoqi Huang Department of Cardiology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaCorrespondence: Zhaoqi Huang, Department of Cardiology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510150, People’s Republic of China, Email zhaoqihuang2007@163.comIntroduction: Adriamycin (ADR) is commonly used in tumor chemotherapy, but its nonreversible cardiotoxicity severely hampers its clinical application. Ferroptosis is an implicated cause of ADR-induced injury. However, the underlying molecular mechanisms remain poorly understood. This study explored whether ferroptosis is a pivotal pathogenic pathway underlying ADR-induced cardiotoxicity and the possible molecular mechanisms involved.Methods: In vivo and in vitro experimental models were used to study the mechanism of ADR-mediated ferroptosis. Ferroptosis levels were examined in mice and human/rat cardiomyocytes. Mechanistically, the expression levels of SLC7A11 and related miRNAs were examined. Bioinformatics prediction and luciferase reporter assays were used to verify the potential interaction between miR-16-5p and SLC7A11. The biological functions and interaction of SLC7A11 and miR-16-5p were investigated in vivo and in vitro.Results: Our study observed that ADR treatment significantly increased ferroptosis levels in vivo and in vitro. Ferroptosis-related pharmacological interventions further confirmed these results. Our data displayed that the SLC7A11 level was significantly decreased in cardiac tissues and cells, while an increased expression level of miR-16-5p was observed. Moreover, upregulation of SLC7A1 and inhibition of miR-16-5p attenuated ADR-induced cardiomyocyte ferroptosis injury. Interactive rescue experiments showed that the protective effects of miR-16-5p inhibition on ADR-induced cardiomyocyte injury were blocked by SLC7A11 knockdown.Discussion: Based on these findings, targeting miR-16-5p could partially reverse the ADR-induced cardiotoxicity by rescuing the SLC7A11 to attenuate ferroptosis. This study presents a pre-clinical basis to identify miR-16-5p/SLC7A11 as a potential treatment target for ADR-induced cardiotoxicity.Keywords: adriamycin, cardiomyocytes, ferroptosis, SLC7A11, miR-16-5phttps://www.dovepress.com/mir-16-5p-regulates-ferroptosis-by-targeting-slc7a11-in-adriamycin-ind-peer-reviewed-fulltext-article-JIRadriamycincardiomyocytesferroptosisslc7a11mir-16-5p |
| spellingShingle | Chen Y Deng Y Chen L Huang Z Yan Y Huang Z miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes Journal of Inflammation Research adriamycin cardiomyocytes ferroptosis slc7a11 mir-16-5p |
| title | miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes |
| title_full | miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes |
| title_fullStr | miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes |
| title_full_unstemmed | miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes |
| title_short | miR-16-5p Regulates Ferroptosis by Targeting SLC7A11 in Adriamycin-Induced Ferroptosis in Cardiomyocytes |
| title_sort | mir 16 5p regulates ferroptosis by targeting slc7a11 in adriamycin induced ferroptosis in cardiomyocytes |
| topic | adriamycin cardiomyocytes ferroptosis slc7a11 mir-16-5p |
| url | https://www.dovepress.com/mir-16-5p-regulates-ferroptosis-by-targeting-slc7a11-in-adriamycin-ind-peer-reviewed-fulltext-article-JIR |
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