Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus
Introduction: Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus. Methods: Extraction was done using the cold maceratio...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2022-07-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-11-409.pdf |
| _version_ | 1818498665852960768 |
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| author | Stephen Kayode S. Ojo Gabriel Temitope Sunmonu Akinwunmi O. Adeoye Christiana Fisayo Akinwunmi Moses Ifeoluwa Obakunle Amos Olakunle Ojerinde Oluwakemi J. Awakan |
| author_facet | Stephen Kayode S. Ojo Gabriel Temitope Sunmonu Akinwunmi O. Adeoye Christiana Fisayo Akinwunmi Moses Ifeoluwa Obakunle Amos Olakunle Ojerinde Oluwakemi J. Awakan |
| author_sort | Stephen Kayode S. Ojo |
| collection | DOAJ |
| description | Introduction: Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus. Methods: Extraction was done using the cold maceration method. The minimum inhibitory concentrations (MIC) of the extracts were determined using the micro-dilution method. Swiss albino rats of 6 sub-groups with 6 animals each (36 animals/organism) were administered with 0.3 ml single oral dose of P. aeruginosa and S. aureus respectively. The animals received treatment for 5 days as follows: 0.5 ml of 5% dimethyl sulphoxide (DMSO) (negative control), 250 mg/kg of amoxicillin (positive control), 2 mg/kg of whole plant extract, 4 mg/kg of whole plant extract, 2 mg/kg of leaf extract, and 4 mg/kg of leaf extract, respectively. The packed cell volume (PCV) and white blood count (WBC) of the animals were determined before and after treatment with histology examination of vital organs. Results: MIC for S. aureus was 2 mg/mL; the mortality in S. aureus group at 2 mg/kg was 66.7%. The PCV values (50.5±0.5, 45.0±1.0, and 50.5±1.5) decreased after infection, and a corresponding increase in the PCV was observed after treatment with the extracts. Also, a significant increase in the WBC values (3.40±0.35, 4.10±0.15, and 3.30±0.40) following infection and a corresponding decrease after treatment were observed. Congestion of vessels in the kidney was also observed. Conclusion: I. asarifolia has a dose-dependent antibacterial and curative activity, and could enhance innate immunity. |
| first_indexed | 2024-12-10T20:18:40Z |
| format | Article |
| id | doaj.art-ae531e716bcd4421bf56f5a97294bc56 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-12-10T20:18:40Z |
| publishDate | 2022-07-01 |
| publisher | Shahrekord University of Medical Sciences |
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| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-ae531e716bcd4421bf56f5a97294bc562022-12-22T01:35:07ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042022-07-0111340941810.34172/jhp.2022.47jhp-42407Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureusStephen Kayode S. Ojo0Gabriel Temitope Sunmonu1Akinwunmi O. Adeoye2Christiana Fisayo Akinwunmi3Moses Ifeoluwa Obakunle4Amos Olakunle Ojerinde5Oluwakemi J. Awakan6Drug Discovery and Infectious Diseases Research Group, Department of Microbiology, Federal University Oye-Ekiti, Ekiti State, NigeriaDrug Discovery and Infectious Diseases Research Group, Department of Microbiology, Federal University Oye-Ekiti, Ekiti State, NigeriaDepartment of Biochemistry, Federal University Oye-Ekiti, Ekiti State, NigeriaDrug Discovery and Infectious Diseases Research Group, Department of Microbiology, Federal University Oye-Ekiti, Ekiti State, NigeriaDrug Discovery and Infectious Diseases Research Group, Department of Microbiology, Federal University Oye-Ekiti, Ekiti State, NigeriaFaculty of Basic Medical Sciences, Federal University Oye-Ekiti, Ekiti State, NigeriaDepartment of Biochemistry, Landmark University, Omu-Aran, Kwara-State, NigeriaIntroduction: Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus. Methods: Extraction was done using the cold maceration method. The minimum inhibitory concentrations (MIC) of the extracts were determined using the micro-dilution method. Swiss albino rats of 6 sub-groups with 6 animals each (36 animals/organism) were administered with 0.3 ml single oral dose of P. aeruginosa and S. aureus respectively. The animals received treatment for 5 days as follows: 0.5 ml of 5% dimethyl sulphoxide (DMSO) (negative control), 250 mg/kg of amoxicillin (positive control), 2 mg/kg of whole plant extract, 4 mg/kg of whole plant extract, 2 mg/kg of leaf extract, and 4 mg/kg of leaf extract, respectively. The packed cell volume (PCV) and white blood count (WBC) of the animals were determined before and after treatment with histology examination of vital organs. Results: MIC for S. aureus was 2 mg/mL; the mortality in S. aureus group at 2 mg/kg was 66.7%. The PCV values (50.5±0.5, 45.0±1.0, and 50.5±1.5) decreased after infection, and a corresponding increase in the PCV was observed after treatment with the extracts. Also, a significant increase in the WBC values (3.40±0.35, 4.10±0.15, and 3.30±0.40) following infection and a corresponding decrease after treatment were observed. Congestion of vessels in the kidney was also observed. Conclusion: I. asarifolia has a dose-dependent antibacterial and curative activity, and could enhance innate immunity.http://herbmedpharmacol.com/PDF/jhp-11-409.pdfmedicinal plantgram negative bacteriagram positive bacteriaantibacterial activityanimal studyhistology |
| spellingShingle | Stephen Kayode S. Ojo Gabriel Temitope Sunmonu Akinwunmi O. Adeoye Christiana Fisayo Akinwunmi Moses Ifeoluwa Obakunle Amos Olakunle Ojerinde Oluwakemi J. Awakan Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus Journal of HerbMed Pharmacology medicinal plant gram negative bacteria gram positive bacteria antibacterial activity animal study histology |
| title | Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus |
| title_full | Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus |
| title_fullStr | Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus |
| title_full_unstemmed | Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus |
| title_short | Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus |
| title_sort | therapeutic potential of ipomoea asarifolia on infected swiss albino rats with pseudomonas aeruginosa and staphylococcus aureus |
| topic | medicinal plant gram negative bacteria gram positive bacteria antibacterial activity animal study histology |
| url | http://herbmedpharmacol.com/PDF/jhp-11-409.pdf |
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