A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds
Multidrug-resistant (MDR) <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the &...
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MDPI AG
2023-06-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/7/942 |
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| author | Deepa Dehari Aiswarya Chaudhuri Dulla Naveen Kumar Rohit Patil Mayank Gangwar Sonam Rastogi Dinesh Kumar Gopal Nath Ashish Kumar Agrawal |
| author_facet | Deepa Dehari Aiswarya Chaudhuri Dulla Naveen Kumar Rohit Patil Mayank Gangwar Sonam Rastogi Dinesh Kumar Gopal Nath Ashish Kumar Agrawal |
| author_sort | Deepa Dehari |
| collection | DOAJ |
| description | Multidrug-resistant (MDR) <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the <i>A. baumannii</i>-specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR <i>A. baumannii</i> clinical strains. Isolated BPABΦ1 were found to belong to the <i>Corticoviridae</i> family, with burst size 102.12 ± 2.65 PFUs per infected host cell. The BPABΦ1 loaded chitosan microparticles were evaluated for quality attributes viz. size, PDI, surface morphology, in vitro release, etc. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application. |
| first_indexed | 2024-03-11T00:45:10Z |
| format | Article |
| id | doaj.art-ae5789185b9a4099a954a14353a34883 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T00:45:10Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-ae5789185b9a4099a954a14353a348832023-11-18T20:52:08ZengMDPI AGPharmaceuticals1424-82472023-06-0116794210.3390/ph16070942A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn WoundsDeepa Dehari0Aiswarya Chaudhuri1Dulla Naveen Kumar2Rohit Patil3Mayank Gangwar4Sonam Rastogi5Dinesh Kumar6Gopal Nath7Ashish Kumar Agrawal8Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaMultidrug-resistant (MDR) <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the <i>A. baumannii</i>-specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR <i>A. baumannii</i> clinical strains. Isolated BPABΦ1 were found to belong to the <i>Corticoviridae</i> family, with burst size 102.12 ± 2.65 PFUs per infected host cell. The BPABΦ1 loaded chitosan microparticles were evaluated for quality attributes viz. size, PDI, surface morphology, in vitro release, etc. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application.https://www.mdpi.com/1424-8247/16/7/942antimicrobial resistancebacteriophagemultidrug resistancewound infectionbacterial biofilm |
| spellingShingle | Deepa Dehari Aiswarya Chaudhuri Dulla Naveen Kumar Rohit Patil Mayank Gangwar Sonam Rastogi Dinesh Kumar Gopal Nath Ashish Kumar Agrawal A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds Pharmaceuticals antimicrobial resistance bacteriophage multidrug resistance wound infection bacterial biofilm |
| title | A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds |
| title_full | A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds |
| title_fullStr | A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds |
| title_full_unstemmed | A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds |
| title_short | A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant <i>Acinetobacter baumannii</i> Bacterial Infections in Burn Wounds |
| title_sort | bacteriophage microgel effectively treats the multidrug resistant i acinetobacter baumannii i bacterial infections in burn wounds |
| topic | antimicrobial resistance bacteriophage multidrug resistance wound infection bacterial biofilm |
| url | https://www.mdpi.com/1424-8247/16/7/942 |
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