Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i>
<i>Burkholderia gladioli</i> has high versatility and adaptability to various ecological niches. Here, we constructed a pan-genome using 14 genome sequences of <i>B. gladioli</i>, which originate from different niches, including gladiolus, rice, humans, and nature. Functional...
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| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1123 |
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| author | Hyun-Hee Lee Jungwook Park Hyejung Jung Young-Su Seo |
| author_facet | Hyun-Hee Lee Jungwook Park Hyejung Jung Young-Su Seo |
| author_sort | Hyun-Hee Lee |
| collection | DOAJ |
| description | <i>Burkholderia gladioli</i> has high versatility and adaptability to various ecological niches. Here, we constructed a pan-genome using 14 genome sequences of <i>B. gladioli</i>, which originate from different niches, including gladiolus, rice, humans, and nature. Functional roles of core and niche-associated genomes were investigated by pathway enrichment analyses. Consequently, we inferred the uniquely important role of niche-associated genomes in (1) selenium availability during competition with gladiolus host; (2) aromatic compound degradation in seed-borne and crude oil-accumulated environments, and (3) stress-induced DNA repair system/recombination in the cystic fibrosis-niche. We also identified the conservation of the rhizomide biosynthetic gene cluster in all the <i>B. gladioli</i> strains and the concentrated distribution of this cluster in human isolates. It was confirmed the absence of complete CRISPR/Cas system in both plant and human pathogenic <i>B. gladioli</i> and the presence of the system in <i>B. gladioli</i> living in nature, possibly reflecting the inverse relationship between CRISPR/Cas system and virulence. |
| first_indexed | 2024-03-10T11:09:37Z |
| format | Article |
| id | doaj.art-ae5833f7be1242eaae42d5ac9d5f1c0f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:09:37Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ae5833f7be1242eaae42d5ac9d5f1c0f2023-11-21T20:56:02ZengMDPI AGMicroorganisms2076-26072021-05-0196112310.3390/microorganisms9061123Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i>Hyun-Hee Lee0Jungwook Park1Hyejung Jung2Young-Su Seo3Department of Integrated Biological Science, Pusan National University, Busan 46241, KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, Korea<i>Burkholderia gladioli</i> has high versatility and adaptability to various ecological niches. Here, we constructed a pan-genome using 14 genome sequences of <i>B. gladioli</i>, which originate from different niches, including gladiolus, rice, humans, and nature. Functional roles of core and niche-associated genomes were investigated by pathway enrichment analyses. Consequently, we inferred the uniquely important role of niche-associated genomes in (1) selenium availability during competition with gladiolus host; (2) aromatic compound degradation in seed-borne and crude oil-accumulated environments, and (3) stress-induced DNA repair system/recombination in the cystic fibrosis-niche. We also identified the conservation of the rhizomide biosynthetic gene cluster in all the <i>B. gladioli</i> strains and the concentrated distribution of this cluster in human isolates. It was confirmed the absence of complete CRISPR/Cas system in both plant and human pathogenic <i>B. gladioli</i> and the presence of the system in <i>B. gladioli</i> living in nature, possibly reflecting the inverse relationship between CRISPR/Cas system and virulence.https://www.mdpi.com/2076-2607/9/6/1123<i>Burkholderia gladioli</i>comparative genomicspan-genomeplant pathogenhuman pathogen |
| spellingShingle | Hyun-Hee Lee Jungwook Park Hyejung Jung Young-Su Seo Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i> Microorganisms <i>Burkholderia gladioli</i> comparative genomics pan-genome plant pathogen human pathogen |
| title | Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i> |
| title_full | Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i> |
| title_fullStr | Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i> |
| title_full_unstemmed | Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i> |
| title_short | Pan-Genome Analysis Reveals Host-Specific Functional Divergences in <i>Burkholderia gladioli</i> |
| title_sort | pan genome analysis reveals host specific functional divergences in i burkholderia gladioli i |
| topic | <i>Burkholderia gladioli</i> comparative genomics pan-genome plant pathogen human pathogen |
| url | https://www.mdpi.com/2076-2607/9/6/1123 |
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