ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds
The seed, a critical organ in higher plants, serves as a primary determinant of agricultural productivity, with its quality directly influencing crop yield. Improper storage conditions can diminish seed vigor, adversely affecting seed germination and seedling establishment. Therefore, understanding...
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MDPI AG
2024-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/13/6/809 |
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| author | Qin Zheng Zhenning Teng Jianhua Zhang Nenghui Ye |
| author_facet | Qin Zheng Zhenning Teng Jianhua Zhang Nenghui Ye |
| author_sort | Qin Zheng |
| collection | DOAJ |
| description | The seed, a critical organ in higher plants, serves as a primary determinant of agricultural productivity, with its quality directly influencing crop yield. Improper storage conditions can diminish seed vigor, adversely affecting seed germination and seedling establishment. Therefore, understanding the seed-aging process and exploring strategies to enhance seed-aging resistance are paramount. In this study, we observed that seed aging during storage leads to a decline in seed vigor and can coincide with the accumulation of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in the radicle, resulting in compromised or uneven germination and asynchronous seedling emergence. We identified the abscisic acid (ABA) catabolism gene, <i>abscisic acid 8′-hydroxylase 2</i> (<i>OsABA8ox2</i>), as significantly induced by aging treatment. Interestingly, transgenic seeds overexpressing OsABA8ox2 exhibited reduced seed vigor, while gene knockout enhanced seed vigor, suggesting its role as a negative regulator. Similarly, seeds pretreated with ABA or diphenyleneiodonium chloride (DPI, an H<sub>2</sub>O<sub>2</sub> inhibitor) showed increased resistance to aging, with more robust early seedling establishment. Both <i>OsABA8ox2</i> mutant seeds and seeds pretreated with ABA or DPI displayed lower H<sub>2</sub>O<sub>2</sub> content during aging treatment. Overall, our findings indicate that ABA mitigates rice seed aging by reducing H<sub>2</sub>O<sub>2</sub> accumulation in the radicle. This study offers valuable germplasm resources and presents a novel approach to enhancing seed resistance against aging. |
| first_indexed | 2024-04-24T17:54:03Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T17:54:03Z |
| publishDate | 2024-03-01 |
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| series | Plants |
| spelling | doaj.art-ae58466421194272a73f326ae009a33a2024-03-27T14:00:43ZengMDPI AGPlants2223-77472024-03-0113680910.3390/plants13060809ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of SeedsQin Zheng0Zhenning Teng1Jianhua Zhang2Nenghui Ye3College of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaSchool of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin 999077, Hong KongCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaThe seed, a critical organ in higher plants, serves as a primary determinant of agricultural productivity, with its quality directly influencing crop yield. Improper storage conditions can diminish seed vigor, adversely affecting seed germination and seedling establishment. Therefore, understanding the seed-aging process and exploring strategies to enhance seed-aging resistance are paramount. In this study, we observed that seed aging during storage leads to a decline in seed vigor and can coincide with the accumulation of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in the radicle, resulting in compromised or uneven germination and asynchronous seedling emergence. We identified the abscisic acid (ABA) catabolism gene, <i>abscisic acid 8′-hydroxylase 2</i> (<i>OsABA8ox2</i>), as significantly induced by aging treatment. Interestingly, transgenic seeds overexpressing OsABA8ox2 exhibited reduced seed vigor, while gene knockout enhanced seed vigor, suggesting its role as a negative regulator. Similarly, seeds pretreated with ABA or diphenyleneiodonium chloride (DPI, an H<sub>2</sub>O<sub>2</sub> inhibitor) showed increased resistance to aging, with more robust early seedling establishment. Both <i>OsABA8ox2</i> mutant seeds and seeds pretreated with ABA or DPI displayed lower H<sub>2</sub>O<sub>2</sub> content during aging treatment. Overall, our findings indicate that ABA mitigates rice seed aging by reducing H<sub>2</sub>O<sub>2</sub> accumulation in the radicle. This study offers valuable germplasm resources and presents a novel approach to enhancing seed resistance against aging.https://www.mdpi.com/2223-7747/13/6/809riceseed agingABAH<sub>2</sub>O<sub>2</sub>germinationseedling establishment |
| spellingShingle | Qin Zheng Zhenning Teng Jianhua Zhang Nenghui Ye ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds Plants rice seed aging ABA H<sub>2</sub>O<sub>2</sub> germination seedling establishment |
| title | ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds |
| title_full | ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds |
| title_fullStr | ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds |
| title_full_unstemmed | ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds |
| title_short | ABA Inhibits Rice Seed Aging by Reducing H<sub>2</sub>O<sub>2</sub> Accumulation in the Radicle of Seeds |
| title_sort | aba inhibits rice seed aging by reducing h sub 2 sub o sub 2 sub accumulation in the radicle of seeds |
| topic | rice seed aging ABA H<sub>2</sub>O<sub>2</sub> germination seedling establishment |
| url | https://www.mdpi.com/2223-7747/13/6/809 |
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