Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia
The distribution of Mycobacterium tuberculosis and scope of tuberculosis morbidity in the world are extremely considerable. Among regions with high burden of tuberculosis (according to the World Health Organization data for 2015) Mongolia is one of the most disadvantaged countries. This research was...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2017-09-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/453 |
| _version_ | 1797237356468633600 |
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| author | E. A. Orlova M. V. Badleeva E. .. Baasansuren N. .. Erdenegerel T. .. Oyuuntuyaa B. .. Buyankhishig B. .. Puntsag D. .. Nyamkhuu S. N. Zhdanova |
| author_facet | E. A. Orlova M. V. Badleeva E. .. Baasansuren N. .. Erdenegerel T. .. Oyuuntuyaa B. .. Buyankhishig B. .. Puntsag D. .. Nyamkhuu S. N. Zhdanova |
| author_sort | E. A. Orlova |
| collection | DOAJ |
| description | The distribution of Mycobacterium tuberculosis and scope of tuberculosis morbidity in the world are extremely considerable. Among regions with high burden of tuberculosis (according to the World Health Organization data for 2015) Mongolia is one of the most disadvantaged countries. This research was continuation of studying of phylogenetic structure of M. tuberculosis for the determination of evolutionary and migration processes in pathogen's northeastern population and was devoted to the study of the spatial distribution of epidemically significant genotypes and subtypes, circulating in central and border (northern and southern) Mongolia. The DNAs of 309 clinical isolates of the pathogen were genotyped by the 24-locus MIRU-VNTR and LSP methods. Strains of the Beijing lineage were additionally analyzed for the presence of specific SNPs and its 24-MIRU-VNTR profiles were compared with reference database of M. Merker et al. (2015). The study indicated significant predominance of strains of the Beijing lineage (subtype CC4). This genotype is characterized by high frequency of clustering, which indicates its epidemic danger and the relatively recent introduction of «successful» strains into the territory of Mongolia. Also, a relatively homogeneous distribution structure of genotypes and subtypes of the pathogen was revealed within the studied region, which differs from the structure of the Russian population of M. tuberculosis by its subtypical composition. Thus, we assuming that CC4-strains of the Beijing lineage spread into Mongolia from neighboring East Asian regions, most likely from the Northern provinces of China (Inner Mongolia) or nearest neighboring countries of Southeast Asia. |
| first_indexed | 2024-03-08T12:18:14Z |
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| id | doaj.art-ae5bcb4adcfc419aaddb69f47ad07c50 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:18:27Z |
| publishDate | 2017-09-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-ae5bcb4adcfc419aaddb69f47ad07c502024-03-28T07:49:18ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962017-09-0125(1)18018510.12737/article_59e85b75b4a0e9.62539831453Phylogeorgaphy of Mycobacterium tuberculosis in central and border MongoliaE. A. Orlova0M. V. Badleeva1E. .. Baasansuren2N. .. Erdenegerel3T. .. Oyuuntuyaa4B. .. Buyankhishig5B. .. Puntsag6D. .. Nyamkhuu7S. N. Zhdanova8Scientific Centre for Family Health and Human Reproduction ProblemsBuryat State UniversityNational Center for Communicable DiseasesNational Center for Communicable DiseasesNational Center for Communicable DiseasesNational Center for Communicable DiseasesNational Center for Communicable DiseasesNational Center for Communicable DiseasesScientific Centre for Family Health and Human Reproduction ProblemsThe distribution of Mycobacterium tuberculosis and scope of tuberculosis morbidity in the world are extremely considerable. Among regions with high burden of tuberculosis (according to the World Health Organization data for 2015) Mongolia is one of the most disadvantaged countries. This research was continuation of studying of phylogenetic structure of M. tuberculosis for the determination of evolutionary and migration processes in pathogen's northeastern population and was devoted to the study of the spatial distribution of epidemically significant genotypes and subtypes, circulating in central and border (northern and southern) Mongolia. The DNAs of 309 clinical isolates of the pathogen were genotyped by the 24-locus MIRU-VNTR and LSP methods. Strains of the Beijing lineage were additionally analyzed for the presence of specific SNPs and its 24-MIRU-VNTR profiles were compared with reference database of M. Merker et al. (2015). The study indicated significant predominance of strains of the Beijing lineage (subtype CC4). This genotype is characterized by high frequency of clustering, which indicates its epidemic danger and the relatively recent introduction of «successful» strains into the territory of Mongolia. Also, a relatively homogeneous distribution structure of genotypes and subtypes of the pathogen was revealed within the studied region, which differs from the structure of the Russian population of M. tuberculosis by its subtypical composition. Thus, we assuming that CC4-strains of the Beijing lineage spread into Mongolia from neighboring East Asian regions, most likely from the Northern provinces of China (Inner Mongolia) or nearest neighboring countries of Southeast Asia.https://www.actabiomedica.ru/jour/article/view/453mycobacterium tuberculosismiru-vntrmycobacterium tuberculosismiru-vntrbeijing lineagephylogeographygenetic diversitytuberculosis in mongolia |
| spellingShingle | E. A. Orlova M. V. Badleeva E. .. Baasansuren N. .. Erdenegerel T. .. Oyuuntuyaa B. .. Buyankhishig B. .. Puntsag D. .. Nyamkhuu S. N. Zhdanova Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia Acta Biomedica Scientifica mycobacterium tuberculosis miru-vntr mycobacterium tuberculosis miru-vntr beijing lineage phylogeography genetic diversity tuberculosis in mongolia |
| title | Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia |
| title_full | Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia |
| title_fullStr | Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia |
| title_full_unstemmed | Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia |
| title_short | Phylogeorgaphy of Mycobacterium tuberculosis in central and border Mongolia |
| title_sort | phylogeorgaphy of mycobacterium tuberculosis in central and border mongolia |
| topic | mycobacterium tuberculosis miru-vntr mycobacterium tuberculosis miru-vntr beijing lineage phylogeography genetic diversity tuberculosis in mongolia |
| url | https://www.actabiomedica.ru/jour/article/view/453 |
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