Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic a...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Microbiology
2012-04-01
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| Series: | Microbiology Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/163 |
| _version_ | 1818978123529584640 |
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| author | YUDHIE ISTIANTO RADEN SETYO ADJI KOESOEMOWIDODO YOSHIO WATANABE HARDANING PRANAMUDA BAMBANG MARWOTO |
| author_facet | YUDHIE ISTIANTO RADEN SETYO ADJI KOESOEMOWIDODO YOSHIO WATANABE HARDANING PRANAMUDA BAMBANG MARWOTO |
| author_sort | YUDHIE ISTIANTO |
| collection | DOAJ |
| description | Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively. |
| first_indexed | 2024-12-20T16:38:38Z |
| format | Article |
| id | doaj.art-ae5ef67a58ab49ffb69dfe7c98af38e0 |
| institution | Directory Open Access Journal |
| issn | 1978-3477 2087-8575 |
| language | English |
| last_indexed | 2024-12-20T16:38:38Z |
| publishDate | 2012-04-01 |
| publisher | Indonesian Society for Microbiology |
| record_format | Article |
| series | Microbiology Indonesia |
| spelling | doaj.art-ae5ef67a58ab49ffb69dfe7c98af38e02022-12-21T19:33:06ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752012-04-016110.5454/mi.6.1.7Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian SoilYUDHIE ISTIANTORADEN SETYO ADJI KOESOEMOWIDODOYOSHIO WATANABEHARDANING PRANAMUDABAMBANG MARWOTOPhenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.https://jurnal.permi.or.id/index.php/mionline/article/view/163phenol methodrare-Actinomycetesselective isolation methodsoil sample. |
| spellingShingle | YUDHIE ISTIANTO RADEN SETYO ADJI KOESOEMOWIDODO YOSHIO WATANABE HARDANING PRANAMUDA BAMBANG MARWOTO Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil Microbiology Indonesia phenol method rare-Actinomycetes selective isolation method soil sample. |
| title | Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil |
| title_full | Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil |
| title_fullStr | Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil |
| title_full_unstemmed | Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil |
| title_short | Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil |
| title_sort | application of phenol pretreatment for the isolation of rare actinomycetes from indonesian soil |
| topic | phenol method rare-Actinomycetes selective isolation method soil sample. |
| url | https://jurnal.permi.or.id/index.php/mionline/article/view/163 |
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