Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening
The identification of new drugs exhibiting reduced adverse side-effects constitutes a great challenge for the next decade. Various steps are needed to screen for good ligand candidates and one of them is the evaluation of their binding properties. New strategies based on fluorescence measurement con...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-01-01
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| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8247/4/1/202/ |
| _version_ | 1828257857520271360 |
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| author | Thierry Durroux Jurriaan M. Zwier Eric Trinquet Orestis Faklaris Martin Cottet Jean-Philippe Pin |
| author_facet | Thierry Durroux Jurriaan M. Zwier Eric Trinquet Orestis Faklaris Martin Cottet Jean-Philippe Pin |
| author_sort | Thierry Durroux |
| collection | DOAJ |
| description | The identification of new drugs exhibiting reduced adverse side-effects constitutes a great challenge for the next decade. Various steps are needed to screen for good ligand candidates and one of them is the evaluation of their binding properties. New strategies based on fluorescence measurement constitute excellent alternatives to the traditional radioactive assays. Less hazardous, faster and cheaper, these methods also exhibit very good sensitivity and can be used on various biological models such as heterologous expression systems or native tissues. |
| first_indexed | 2024-04-13T02:46:58Z |
| format | Article |
| id | doaj.art-ae64c31ca6164ee9b727fd83d074ad66 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-04-13T02:46:58Z |
| publishDate | 2011-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-ae64c31ca6164ee9b727fd83d074ad662022-12-22T03:05:59ZengMDPI AGPharmaceuticals1424-82472011-01-014120221410.3390/ph4010202Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug ScreeningThierry DurrouxJurriaan M. ZwierEric TrinquetOrestis FaklarisMartin CottetJean-Philippe PinThe identification of new drugs exhibiting reduced adverse side-effects constitutes a great challenge for the next decade. Various steps are needed to screen for good ligand candidates and one of them is the evaluation of their binding properties. New strategies based on fluorescence measurement constitute excellent alternatives to the traditional radioactive assays. Less hazardous, faster and cheaper, these methods also exhibit very good sensitivity and can be used on various biological models such as heterologous expression systems or native tissues.http://www.mdpi.com/1424-8247/4/1/202/screening assayfluorescencelanthanidestime resolved FRETGPCR |
| spellingShingle | Thierry Durroux Jurriaan M. Zwier Eric Trinquet Orestis Faklaris Martin Cottet Jean-Philippe Pin Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening Pharmaceuticals screening assay fluorescence lanthanides time resolved FRET GPCR |
| title | Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening |
| title_full | Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening |
| title_fullStr | Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening |
| title_full_unstemmed | Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening |
| title_short | Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening |
| title_sort | original fluorescent ligand based assays open new perspectives in g protein coupled receptor drug screening |
| topic | screening assay fluorescence lanthanides time resolved FRET GPCR |
| url | http://www.mdpi.com/1424-8247/4/1/202/ |
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