Identification and Characterization of Rat RhoA Gene Promoter
RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5′-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319309843 |
| _version_ | 1819291668326645760 |
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| author | Yoshihiko Chiba Kumiko Goto Kimihiko Matsusue Shioko Kimura Miwa Misawa |
| author_facet | Yoshihiko Chiba Kumiko Goto Kimihiko Matsusue Shioko Kimura Miwa Misawa |
| author_sort | Yoshihiko Chiba |
| collection | DOAJ |
| description | RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5′-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13)–stimulated cells revealed a significant promoter activity at 238- to 166-bp upstream of the transcription-initiation site, which contains a signal transducer and activation of transcription (STAT) 6–binding region. The IL-13–induced increase in luciferase activity was inhibited by a STAT6 inhibitor, AS1517499, or a Janus kinases (JAKs) inhibitor, JAK Inhibitor-I, but not by tyrphostin-AG490, WHI-P131, or tyrphostin-AG9 (selective JAK2, JAK3, and Tyk2 inhibitors, respectively). Thus, rat BSM rhoA expression may have causal relation to the IL-13–JAK1–STAT6 signaling. Keywords:: RhoA, transcriptional regulation, bronchial smooth muscle |
| first_indexed | 2024-12-24T03:42:18Z |
| format | Article |
| id | doaj.art-ae66e2dd33054107be1d7ae25defd74c |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-24T03:42:18Z |
| publishDate | 2010-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-ae66e2dd33054107be1d7ae25defd74c2022-12-21T17:16:51ZengElsevierJournal of Pharmacological Sciences1347-86132010-01-011124467472Identification and Characterization of Rat RhoA Gene PromoterYoshihiko Chiba0Kumiko Goto1Kimihiko Matsusue2Shioko Kimura3Miwa Misawa4Department of Pharmacology, School of Pharmacy, Hoshi University, Tokyo 142-8501, Japan; Corresponding author. chiba@hoshi.ac.jpDepartment of Pharmacology, School of Pharmacy, Hoshi University, Tokyo 142-8501, JapanFaculty of Pharmaceutical Science, Fukuoka University, Fukuoka 814-0180, JapanLaboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20814, USADepartment of Pharmacology, School of Pharmacy, Hoshi University, Tokyo 142-8501, JapanRhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5′-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13)–stimulated cells revealed a significant promoter activity at 238- to 166-bp upstream of the transcription-initiation site, which contains a signal transducer and activation of transcription (STAT) 6–binding region. The IL-13–induced increase in luciferase activity was inhibited by a STAT6 inhibitor, AS1517499, or a Janus kinases (JAKs) inhibitor, JAK Inhibitor-I, but not by tyrphostin-AG490, WHI-P131, or tyrphostin-AG9 (selective JAK2, JAK3, and Tyk2 inhibitors, respectively). Thus, rat BSM rhoA expression may have causal relation to the IL-13–JAK1–STAT6 signaling. Keywords:: RhoA, transcriptional regulation, bronchial smooth musclehttp://www.sciencedirect.com/science/article/pii/S1347861319309843 |
| spellingShingle | Yoshihiko Chiba Kumiko Goto Kimihiko Matsusue Shioko Kimura Miwa Misawa Identification and Characterization of Rat RhoA Gene Promoter Journal of Pharmacological Sciences |
| title | Identification and Characterization of Rat RhoA Gene Promoter |
| title_full | Identification and Characterization of Rat RhoA Gene Promoter |
| title_fullStr | Identification and Characterization of Rat RhoA Gene Promoter |
| title_full_unstemmed | Identification and Characterization of Rat RhoA Gene Promoter |
| title_short | Identification and Characterization of Rat RhoA Gene Promoter |
| title_sort | identification and characterization of rat rhoa gene promoter |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319309843 |
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