Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
Abstract Background Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is th...
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| Format: | Article |
| Language: | English |
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BMC
2015-09-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-015-1109-6 |
| _version_ | 1827933718352756736 |
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| author | Francisco Panzera Sebastián Pita Julieta Nattero Yanina Panzera Cleber Galvão Tamara Chavez Antonieta Rojas De Arias Lourdes Cardozo Téllez François Noireau |
| author_facet | Francisco Panzera Sebastián Pita Julieta Nattero Yanina Panzera Cleber Galvão Tamara Chavez Antonieta Rojas De Arias Lourdes Cardozo Téllez François Noireau |
| author_sort | Francisco Panzera |
| collection | DOAJ |
| description | Abstract Background Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published. |
| first_indexed | 2024-03-13T07:28:41Z |
| format | Article |
| id | doaj.art-ae69724a42ad4dc79c944f7c28406ad6 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:28:41Z |
| publishDate | 2015-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-ae69724a42ad4dc79c944f7c28406ad62023-06-04T11:13:59ZengBMCParasites & Vectors1756-33052015-09-018111010.1186/s13071-015-1109-6Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markersFrancisco Panzera0Sebastián Pita1Julieta Nattero2Yanina Panzera3Cleber Galvão4Tamara Chavez5Antonieta Rojas De Arias6Lourdes Cardozo Téllez7François Noireau8Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la RepúblicaSección Genética Evolutiva, Facultad de Ciencias, Universidad de la RepúblicaCátedra Introducción a la Biología, Facultad de Ciencias Exactas Físicas y Naturales, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT) CONICET, Universidad Nacional de CórdobaSección Genética Evolutiva, Facultad de Ciencias, Universidad de la RepúblicaLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos (LNIRTT), Instituto OswaldoInstituto Nacional de Laboratorios de Salud (INLASA), Laboratorio de Entomología MédicaCentro para el Desarrollo de la Investigación Científica (CEDIC)/Díaz Gill Medicina Laboratorial/Fundación Moisés BertoniLaboratorio de Biotecnología, Centro de Investigación Hernando Bertoni, Instituto Paraguayo de Tecnología AgrariaInteractions hôtes-vecteurs-parasites dans les infections par trypanosomatidae (INTERTRYP), Institut de Recherche pour le Développement (IRD)Abstract Background Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published.https://doi.org/10.1186/s13071-015-1109-6Sordida subcomplex speciesTriatominaeChagas disease vectorsCryptic speciesHolocentric chromosomesFISH |
| spellingShingle | Francisco Panzera Sebastián Pita Julieta Nattero Yanina Panzera Cleber Galvão Tamara Chavez Antonieta Rojas De Arias Lourdes Cardozo Téllez François Noireau Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers Parasites & Vectors Sordida subcomplex species Triatominae Chagas disease vectors Cryptic species Holocentric chromosomes FISH |
| title | Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_full | Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_fullStr | Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_full_unstemmed | Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_short | Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_sort | cryptic speciation in the triatoma sordida subcomplex hemiptera reduviidae revealed by chromosomal markers |
| topic | Sordida subcomplex species Triatominae Chagas disease vectors Cryptic species Holocentric chromosomes FISH |
| url | https://doi.org/10.1186/s13071-015-1109-6 |
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