Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors
The finding of molecules associated with aging is important for the prevention of chronic degenerative diseases and for longevity strategies. MicroRNAs (miRNAs) are post-transcriptional regulators involved in many biological processes and miR-146b-5p has been shown to be involved in different degene...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/17/13163 |
| _version_ | 1797582473809362944 |
|---|---|
| author | Luca Dalle Carbonare Arianna Minoia Michele Braggio Jessica Bertacco Francesca Cristiana Piritore Sharazed Zouari Anna Vareschi Rossella Elia Ermes Vedovi Cristina Scumà Matilde Carlucci Lekhana Bhandary Monica Mottes Maria Grazia Romanelli Maria Teresa Valenti |
| author_facet | Luca Dalle Carbonare Arianna Minoia Michele Braggio Jessica Bertacco Francesca Cristiana Piritore Sharazed Zouari Anna Vareschi Rossella Elia Ermes Vedovi Cristina Scumà Matilde Carlucci Lekhana Bhandary Monica Mottes Maria Grazia Romanelli Maria Teresa Valenti |
| author_sort | Luca Dalle Carbonare |
| collection | DOAJ |
| description | The finding of molecules associated with aging is important for the prevention of chronic degenerative diseases and for longevity strategies. MicroRNAs (miRNAs) are post-transcriptional regulators involved in many biological processes and miR-146b-5p has been shown to be involved in different degenerative diseases. However, miR-146b-5p modulation has not been evaluated in mesenchymal stem cells (MSCs) commitment or during aging. Therefore, the modulation of miR-146b-5p in the commitment and differentiation of mesenchymal cells as well as during maturation and aging in zebrafish model were analyzed. In addition, circulating miR-146b-5p was evaluated in human subjects at different age ranges. Thus, the role of physical activity in the modulation of miR-146b-5p was also investigated. To achieve these aims, RT (real-time)-PCR, Western blot, cell transfections, and three-dimensional (3D) culture techniques were applied. Our findings show that miR-146b-5p expression drives MSCs to adipogenic differentiation and increases during zebrafish maturation and aging. In addition, miR-146b-5p expression is higher in females compared to males and it is associated with the aging in humans. Interestingly, we also observed that the physical activity of walking downregulates circulating miR-146b-5p levels in human females and increases the number of chondroprogenitors. In conclusion, miR-146b-5p can be considered an age-related marker and can represent a useful marker for identifying strategies, such as physical activity, aimed at counteracting the degenerative processes of aging. |
| first_indexed | 2024-03-10T23:22:50Z |
| format | Article |
| id | doaj.art-ae69e2db20ab491eafd1b5fca330ee01 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:22:50Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ae69e2db20ab491eafd1b5fca330ee012023-11-19T08:13:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171316310.3390/ijms241713163Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic ProgenitorsLuca Dalle Carbonare0Arianna Minoia1Michele Braggio2Jessica Bertacco3Francesca Cristiana Piritore4Sharazed Zouari5Anna Vareschi6Rossella Elia7Ermes Vedovi8Cristina Scumà9Matilde Carlucci10Lekhana Bhandary11Monica Mottes12Maria Grazia Romanelli13Maria Teresa Valenti14Department of Engineering for Innovative Medicine, University of Verona, 37100 Verona, ItalyDepartment of Engineering for Innovative Medicine, University of Verona, 37100 Verona, ItalyDepartment of Engineering for Innovative Medicine, University of Verona, 37100 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, ItalyDepartment of Engineering for Innovative Medicine, University of Verona, 37100 Verona, ItalyDepartment of Engineering for Innovative Medicine, University of Verona, 37100 Verona, ItalyDepartment of Engineering for Innovative Medicine, University of Verona, 37100 Verona, ItalyRecovery and Functional Rehabilitation, Integrated University Hospital of Verona, 37100 Verona, ItalyRecovery and Functional Rehabilitation, Integrated University Hospital of Verona, 37100 Verona, ItalyHealth Directorate, Integrated University Hospital of Verona, 37100 Verona, ItalyFlaskworks, LLC., Boston, MA 02118, USADepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100 Verona, ItalyThe finding of molecules associated with aging is important for the prevention of chronic degenerative diseases and for longevity strategies. MicroRNAs (miRNAs) are post-transcriptional regulators involved in many biological processes and miR-146b-5p has been shown to be involved in different degenerative diseases. However, miR-146b-5p modulation has not been evaluated in mesenchymal stem cells (MSCs) commitment or during aging. Therefore, the modulation of miR-146b-5p in the commitment and differentiation of mesenchymal cells as well as during maturation and aging in zebrafish model were analyzed. In addition, circulating miR-146b-5p was evaluated in human subjects at different age ranges. Thus, the role of physical activity in the modulation of miR-146b-5p was also investigated. To achieve these aims, RT (real-time)-PCR, Western blot, cell transfections, and three-dimensional (3D) culture techniques were applied. Our findings show that miR-146b-5p expression drives MSCs to adipogenic differentiation and increases during zebrafish maturation and aging. In addition, miR-146b-5p expression is higher in females compared to males and it is associated with the aging in humans. Interestingly, we also observed that the physical activity of walking downregulates circulating miR-146b-5p levels in human females and increases the number of chondroprogenitors. In conclusion, miR-146b-5p can be considered an age-related marker and can represent a useful marker for identifying strategies, such as physical activity, aimed at counteracting the degenerative processes of aging.https://www.mdpi.com/1422-0067/24/17/13163miR-146b-5pdifferentiationcirculating microRNAsaging |
| spellingShingle | Luca Dalle Carbonare Arianna Minoia Michele Braggio Jessica Bertacco Francesca Cristiana Piritore Sharazed Zouari Anna Vareschi Rossella Elia Ermes Vedovi Cristina Scumà Matilde Carlucci Lekhana Bhandary Monica Mottes Maria Grazia Romanelli Maria Teresa Valenti Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors International Journal of Molecular Sciences miR-146b-5p differentiation circulating microRNAs aging |
| title | Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors |
| title_full | Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors |
| title_fullStr | Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors |
| title_full_unstemmed | Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors |
| title_short | Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors |
| title_sort | modulation of mir 146b expression during aging and the impact of physical activity on its expression and chondrogenic progenitors |
| topic | miR-146b-5p differentiation circulating microRNAs aging |
| url | https://www.mdpi.com/1422-0067/24/17/13163 |
| work_keys_str_mv | AT lucadallecarbonare modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT ariannaminoia modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT michelebraggio modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT jessicabertacco modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT francescacristianapiritore modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT sharazedzouari modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT annavareschi modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT rossellaelia modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT ermesvedovi modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT cristinascuma modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT matildecarlucci modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT lekhanabhandary modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT monicamottes modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT mariagraziaromanelli modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors AT mariateresavalenti modulationofmir146bexpressionduringagingandtheimpactofphysicalactivityonitsexpressionandchondrogenicprogenitors |