Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery
Abstract Objective Nosocomial bacterial meningitis is one of the major complications after neurosurgery. We performed nanopore 16S amplicon sequencing from cerebrospinal fluid (CSF) to evaluate bacterial meningitis in patients who underwent neurosurgery. Methods Among the patients who visited the ne...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-03-01
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| Series: | Annals of Clinical and Translational Neurology |
| Online Access: | https://doi.org/10.1002/acn3.51517 |
| _version_ | 1828882743894736896 |
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| author | Yoonhyuk Jang Seondeuk Kim Narae Kim Hyoshin Son Eun Jin Ha Eun Jung Koh Ji Hoon Phi Chul‐Kee Park Jeong Eun Kim Seung‐Ki Kim Sang Kun Lee Won‐Sang Cho Jangsup Moon Kon Chu |
| author_facet | Yoonhyuk Jang Seondeuk Kim Narae Kim Hyoshin Son Eun Jin Ha Eun Jung Koh Ji Hoon Phi Chul‐Kee Park Jeong Eun Kim Seung‐Ki Kim Sang Kun Lee Won‐Sang Cho Jangsup Moon Kon Chu |
| author_sort | Yoonhyuk Jang |
| collection | DOAJ |
| description | Abstract Objective Nosocomial bacterial meningitis is one of the major complications after neurosurgery. We performed nanopore 16S amplicon sequencing from cerebrospinal fluid (CSF) to evaluate bacterial meningitis in patients who underwent neurosurgery. Methods Among the patients who visited the neurosurgery department of Seoul National University Hospital between July 2017 and June 2020, those with clinically suspected bacterial meningitis were included. 16S rDNA PCR was performed from the CSF, and nanopore sequencing was performed for up to 3 h. The reads were aligned to the BLAST database. In each case, the culture and the 16S rRNA gene amplicon analysis were simultaneously performed and compared with each other, and we retrospectively reviewed the medical records. Genuine infection was determined by the identical results between conventional culture study and the sequencing, or clinically determined in cases with inconsistent results between the two methods. Results Of the 285 samples obtained from 178 patients who had 16S rDNA PCR, 41 samples (14.4%) were diagnosed with genuine infection. A total of 56.1% (23/41) of the samples with genuine infection showed a false‐negative culture test. In particular, 16S amplicon sequencing was useful in evaluating patients at the initial tests who had infection with intraventricular hemorrhage (Culture false‐negative rate = 100%), subarachnoid hemorrhage (Culture false‐negative rate = 77.8%), and systemic cancer (Culture false‐negative rate = 100%), which are risk factors for central fever. Moreover, 16S amplicon sequencing could suggest the possibility of persistent bacterial meningitis in empirical antibiotic use. Conclusion CSF nanopore 16S sequencing was more effective than conventional CSF culture studies in postoperative bacterial meningitis and may contribute to evidence‐based decisions for antibiotic maintenance and discontinuation. |
| first_indexed | 2024-12-13T10:34:09Z |
| format | Article |
| id | doaj.art-ae6a5641faca4e988f29fa489db29595 |
| institution | Directory Open Access Journal |
| issn | 2328-9503 |
| language | English |
| last_indexed | 2024-12-13T10:34:09Z |
| publishDate | 2022-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Annals of Clinical and Translational Neurology |
| spelling | doaj.art-ae6a5641faca4e988f29fa489db295952022-12-21T23:50:46ZengWileyAnnals of Clinical and Translational Neurology2328-95032022-03-019331232510.1002/acn3.51517Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgeryYoonhyuk Jang0Seondeuk Kim1Narae Kim2Hyoshin Son3Eun Jin Ha4Eun Jung Koh5Ji Hoon Phi6Chul‐Kee Park7Jeong Eun Kim8Seung‐Ki Kim9Sang Kun Lee10Won‐Sang Cho11Jangsup Moon12Kon Chu13Department of Neurology Seoul National University College of Medicine Seoul National University Hospital Seoul South KoreaDepartment of Neurology Seoul National University College of Medicine Seoul National University Hospital Seoul South KoreaDepartment of Neurology Seoul National University College of Medicine Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurology Seoul National University College of Medicine Seoul National University Hospital Seoul South KoreaDepartment of Neurosurgery Seoul National University College of Medicine, Seoul National University Hospital Seoul South KoreaDepartment of Neurology Seoul National University College of Medicine Seoul National University Hospital Seoul South KoreaDepartment of Neurology Seoul National University College of Medicine Seoul National University Hospital Seoul South KoreaAbstract Objective Nosocomial bacterial meningitis is one of the major complications after neurosurgery. We performed nanopore 16S amplicon sequencing from cerebrospinal fluid (CSF) to evaluate bacterial meningitis in patients who underwent neurosurgery. Methods Among the patients who visited the neurosurgery department of Seoul National University Hospital between July 2017 and June 2020, those with clinically suspected bacterial meningitis were included. 16S rDNA PCR was performed from the CSF, and nanopore sequencing was performed for up to 3 h. The reads were aligned to the BLAST database. In each case, the culture and the 16S rRNA gene amplicon analysis were simultaneously performed and compared with each other, and we retrospectively reviewed the medical records. Genuine infection was determined by the identical results between conventional culture study and the sequencing, or clinically determined in cases with inconsistent results between the two methods. Results Of the 285 samples obtained from 178 patients who had 16S rDNA PCR, 41 samples (14.4%) were diagnosed with genuine infection. A total of 56.1% (23/41) of the samples with genuine infection showed a false‐negative culture test. In particular, 16S amplicon sequencing was useful in evaluating patients at the initial tests who had infection with intraventricular hemorrhage (Culture false‐negative rate = 100%), subarachnoid hemorrhage (Culture false‐negative rate = 77.8%), and systemic cancer (Culture false‐negative rate = 100%), which are risk factors for central fever. Moreover, 16S amplicon sequencing could suggest the possibility of persistent bacterial meningitis in empirical antibiotic use. Conclusion CSF nanopore 16S sequencing was more effective than conventional CSF culture studies in postoperative bacterial meningitis and may contribute to evidence‐based decisions for antibiotic maintenance and discontinuation.https://doi.org/10.1002/acn3.51517 |
| spellingShingle | Yoonhyuk Jang Seondeuk Kim Narae Kim Hyoshin Son Eun Jin Ha Eun Jung Koh Ji Hoon Phi Chul‐Kee Park Jeong Eun Kim Seung‐Ki Kim Sang Kun Lee Won‐Sang Cho Jangsup Moon Kon Chu Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery Annals of Clinical and Translational Neurology |
| title | Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery |
| title_full | Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery |
| title_fullStr | Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery |
| title_full_unstemmed | Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery |
| title_short | Nanopore 16S sequencing enhances the detection of bacterial meningitis after neurosurgery |
| title_sort | nanopore 16s sequencing enhances the detection of bacterial meningitis after neurosurgery |
| url | https://doi.org/10.1002/acn3.51517 |
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